LOCUS       sequence-253748-        2763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC8A2
FEATURES             Location/Qualifiers
     source          1..2763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcccccc tggccctggt gggcgtgacc ctgctgctgg cagcaccacc ttgcagcgga
       61 gcagcaaccc ctacaccatc cctgccacca cctccagcaa acgacagcga tacctccaca
      121 ggcggctgcc agggctctta caggtgtcag ccaggcgtgc tgctgcccgt gtgggagcca
      181 gacgatccaa gcctgggcga caaggcagca agggcagtgg tgtatttcgt ggccatggtg
      241 tacatgtttc tgggcgtgag catcatcgcc gatcgcttta tggccgccat cgaagtgatc
      301 acatccaagg agaaggagat caccatcaca aaggccaatg gcgagaccag cgtgggcaca
      361 gtgcgcatct ggaacgagac cgtgtccaat ctgacactga tggcactggg aagctccgcc
      421 ccagagatcc tgctgtccgt gatcgaggtg tgcggccaca acttccaggc cggcgagctg
      481 ggaccaggaa ccatcgtggg atccgccgcc ttcaatatgt ttgtggtcat cgccgtgtgc
      541 atctatgtga tccccgccgg cgagtctcgg aagatcaagc acctgagagt gttctttgtg
      601 acagcctcct ggtctatctt cgcctacgtg tggctgtacc tgatcctggc cgtgtttagc
      661 cctggagtgg tgcaagtgtg ggaggccctg ctgaccctgg tgttctttcc agtgtgcgtg
      721 gtgttcgcct ggatggccga caagcggctg ctgttttaca agtacgtgta caagcggtac
      781 cggacagatc ctagatccgg aatcatcatc ggagcagagg gcgacccacc taagtctatc
      841 gagctggatg gcacctttgt gggagcagag gcaccaggcg agctgggagg actgggacca
      901 ggacctgcag aggcccggga gctggacgcc tcccggagag aagtgatcca gatcctgaag
      961 gatctgaagc agaagcaccc agacaaggat ctggagcagc tggtgggcat cgccaactac
     1021 tatgccctgc tgcaccagca gaagtctaga gccttctata ggatccaggc aaccaggctg
     1081 atgacaggag caggcaatgt gctgaggcgc cacgccgccg acgccagcag gagagcagca
     1141 ccagcagagg gagcaggcga ggatgaggac gatggcgcct cccgcatctt ctttgagcct
     1201 tctctgtacc actgcctgga gaactgtggc tctgtgctgc tgagcgtgac ctgtcaggga
     1261 ggagagggca atagcacctt ttatgtggac tacaggacag aggatggctc cgccaaggcc
     1321 ggctctgact atgagtacag cgagggcacc ctggtgttta agcctggcga gacacagaag
     1381 gagctgcgca tcggcatcat cgacgatgac atcttcgagg aggatgagca cttctttgtg
     1441 cggctgctga acctgagagt gggcgacgcc cagggcatgt tcgagccaga tggaggaggc
     1501 agacccaagg gccggctggt ggcccctctg ctggccaccg tgacaatcct ggatgacgat
     1561 cacgccggca tcttctcttt tcaggaccgg ctgctgcacg tgagcgagtg catgggcaca
     1621 gtggatgtga gagtggtgag gtctagcgga gcaagaggaa ccgtgaggct gccatatcgc
     1681 accgtggacg gaacagcaag aggaggagga gtgcactacg aggatgcctg tggcgagctg
     1741 gagttcggcg acgatgagac catgaagaca ctgcaggtga agatcgtgga cgatgaggag
     1801 tatgagaaga aggacaactt ctttatcgag ctgggccagc cacagtggct gaagaggggc
     1861 atctccgccc tgctgctgaa tcagggcgac ggcgatagga agctgaccgc agaggaggag
     1921 gaggcaagga ggatcgcaga gatgggcaag cccgtgctgg gagagaattg ccggctggaa
     1981 gtgatcatcg aggagtctta cgactttaag aacaccgtgg ataagctgat caagaagaca
     2041 aacctggccc tggtcatcgg cacccactct tggagagagc agttcctgga ggcaatcacc
     2101 gtgagcgccg gcgacgaaga ggaggaggag gatggaagca gggaggagcg gctgccatcc
     2161 tgcttcgact atgtgatgca cttcctgaca gtgttttgga aggtgctgtt tgcctgcgtg
     2221 ccaccaaccg agtactgcca cggatgggcc tgtttcggcg tgtccatcct ggtcatcggc
     2281 ctgctgacag ccctgatcgg cgacctggca tcccacttcg gatgcaccgt gggcctgaag
     2341 gattctgtga acgcagtggt gttcgtggcc ctgggcacct ctatccctga cacatttgcc
     2401 agcaaggtgg ccgccctgca ggaccagtgt gcagatgcct ccatcggcaa tgtgaccggc
     2461 tctaacgccg tgaacgtgtt cctgggactg ggagtggcat ggagcgtggc agccgtgtat
     2521 tgggcagtgc agggcaggcc cttcgaggtg cgcaccggca cactggcctt ctccgtgacc
     2581 ctgtttacag tgttcgcctt tgtgggcatc gccgtgctgc tgtaccggag aaggcctcac
     2641 atcggaggag agctgggagg acctagggga ccaaagctgg caaccacagc cctgttcctg
     2701 ggcctgtggc tgctgtatat cctgtttgcc agcctggagg cctactgtca catcagaggc
     2761 ttc
//
