LOCUS       sequence-253760-        2733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC4A1
FEATURES             Location/Qualifiers
     source          1..2733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaggagc tgcaggacga ttacgaggac atgatggagg agaacctgga gcaggaggag
       61 tatgaggacc ccgatatccc tgagtcccag atggaggagc cagcagcaca cgataccgag
      121 gcaaccgcca cagactacca caccacatct caccccggca cacacaaggt gtatgtggag
      181 ctgcaggagc tggtcatgga tgagaagaat caggagctgc ggtggatgga ggccgccaga
      241 tgggtgcagc tggaggagaa cctgggagag aatggagcat ggggcagacc ccacctgtcc
      301 cacctgacat tttggtctct gctggagctg cggagagtgt tcaccaaggg cacagtgctg
      361 ctggacctgc aggagacctc tctggcagga gtggcaaacc agctgctgga caggtttatc
      421 ttcgaggatc agatcaggcc tcaggacagg gaggagctgc tgcgcgccct gctgctgaag
      481 cactcccacg caggagagct ggaggccctg ggaggcgtga agccagccgt gctgacaaga
      541 tctggcgatc caagccagcc actgctgcct cagcacagct ccctggagac ccagctgttc
      601 tgcgagcagg gcgacggagg aacagaggga cactccccat ctggcatcct ggagaagatc
      661 ccccctgata gcgaggccac cctggtgctg gtgggaaggg cagactttct ggagcagccc
      721 gtgctgggct tcgtgagact gcaggaggca gcagagctgg aggcagtgga gctgccagtg
      781 cctatccggt ttctgttcgt gctgctggga ccagaggcac cacacatcga ttacacccag
      841 ctgggaagag cagcagccac actgatgagc gagcgggtgt tcagaatcga cgcctatatg
      901 gcacagtcca ggggagagct gctgcactct ctggagggct ttctggattg ttccctggtg
      961 ctgccaccaa ccgacgcacc aagcgagcag gccctgctgt ccctggtgcc agtgcagagg
     1021 gagctgctga ggcgccggta ccagtctagc cctgccaagc cagattcctc tttctataag
     1081 ggactggacc tgaatggagg accagacgat cctctgcagc agacaggcca gctgtttggc
     1141 ggcctggtga gggacatcag aaggcgctac ccttactatc tgagcgacat caccgatgcc
     1201 ttctccccac aggtgctggc agccgtgatc tttatctatt tcgccgccct gagcccagca
     1261 atcacctttg gcggactgct gggcgagaag acacgcaacc agatgggcgt gtctgagctg
     1321 ctgatcagca cagccgtgca gggcatcctg ttcgcactgc tgggagcaca gccactgctg
     1381 gtggtgggct tcagcggccc tctgctggtg tttgaggagg ccttcttttc cttctgcgag
     1441 accaatggcc tggagtacat cgtgggcagg gtgtggatcg gcttctggct gatcctgctg
     1501 gtggtgctgg tggtggcctt tgagggctct ttcctggtgc ggtttatcag cagatacacc
     1561 caggagatct tttccttcct gatctctctg atcttcatct atgagacctt cagcaagctg
     1621 atcaagatct tccaggatca ccccctgcag aagacctaca actataatgt gctgatggtg
     1681 ccaaagccac agggacctct gccaaacacc gccctgctga gcctggtgct gatggcaggc
     1741 acattctttt tcgccatgat gctgcgcaag ttcaagaata gctcctactt tcccggcaag
     1801 ctgcggagag tgatcggcga tttcggcgtg cctatcagca tcctgatcat ggtgctggtg
     1861 gactttttca tccaggatac ctatacacag aagctgtccg tgcctgacgg ctttaaggtg
     1921 tctaactcta gcgccagggg atgggtcatc caccctctgg gactgcgctc cgagttccca
     1981 atctggatga tgtttgcctc tgccctgcct gccctgctgg tgttcatcct gatctttctg
     2041 gagagccaga tcaccacact gatcgtgtcc aagccagagc ggaagatggt gaagggctcc
     2101 ggctttcacc tggacctgct gctggtggtg ggaatgggag gagtggccgc cctgttcgga
     2161 atgccatggc tgtctgccac cacagtgaga agcgtgaccc acgccaatgc cctgacagtg
     2221 atgggcaagg catctacccc tggagcagca gcacagatcc aggaggtgaa ggagcagagg
     2281 atcagcggac tgctggtggc cgtgctggtg ggactgtcta tcctgatgga gcctatcctg
     2341 agccgcatcc cactggccgt gctgtttggc atcttcctgt acatgggcgt gaccagcctg
     2401 tccggcatcc agctgtttga taggatcctg ctgctgttca agcctccaaa gtaccaccca
     2461 gacgtgccct atgtgaagag ggtgaagacc tggcgcatgc acctgttcac aggcatccag
     2521 atcatctgcc tggccgtgct gtgggtggtg aagagcaccc cagcatccct ggccctgcct
     2581 tttgtgctga tcctgacagt gccactgagg cgcgtgctgc tgccactgat cttccggaac
     2641 gtggagctgc agtgtctgga tgccgacgat gccaaggcca cctttgacga ggaggagggc
     2701 agagacgagt acgatgaggt ggccatgccc gtg
//
