LOCUS       sequence-253786-        1992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC5A1
FEATURES             Location/Qualifiers
     source          1..1992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggacagct ccacctggtc tcctaagacc acagccgtga cccgcccagt ggagacacac
       61 gagctgatcc ggaacgccgc cgatatcagc atcatcgtga tctacttcgt ggtggtcatg
      121 gcagtgggac tgtgggccat gttttctacc aatagaggca cagtgggcgg cttctttctg
      181 gcaggcagga gcatggtgtg gtggccaatc ggagcaagcc tgttcgcctc caacatcgga
      241 tctggacact ttgtgggact ggcaggaacc ggagcagcat ccggcatcgc catcggcggc
      301 ttcgagtgga atgcactggt gctggtggtg gtgctgggat ggctgtttgt gccaatctac
      361 atcaaggccg gcgtggtgac catgcccgag tatctgagga agcggttcgg aggacagagg
      421 atccaggtgt acctgagcct gctgtccctg ctgctgtata tctttacaaa gatctctgcc
      481 gacatcttca gcggcgccat ctttatcaac ctggccctgg gcctgaatct gtacctggcc
      541 atcttcctgc tgctggccat caccgccctg tacaccatca caggcggcct ggccgccgtg
      601 atctataccg acacactgca gaccgtgatc atgctggtgg gctccctgat cctgacaggc
      661 ttcgcctttc acgaagtggg cggctacgat gccttcatgg agaagtatat gaaggccatc
      721 cccacaatcg tgtccgacgg caacaccaca tttcaggaga agtgctacac ccctagagcc
      781 gattctttcc acatctttag ggacccactg acaggcgatc tgccatggcc cggcttcatc
      841 tttggcatgt ctatcctgac cctgtggtat tggtgcacag atcaggtcat cgtgcagaga
      901 tgtctgtccg ccaagaatat gtctcacgtg aagggcggct gcatcctgtg cggctacctg
      961 aagctgatgc caatgttcat catggtcatg cccggcatga tcagcaggat cctgtatacc
     1021 gagaagatcg cctgcgtggt gccttccgag tgcgagaagt actgtggcac caaagtgggc
     1081 tgtacaaaca tcgcctatcc caccctggtg gtggagctga tgcctaatgg cctgcgcggc
     1141 ctgatgctga gcgtgatgct ggcctccctg atgtctagcc tgacatccat cttcaactcc
     1201 gcctctaccc tgtttacaat ggacatctac gccaaggtgc ggaagagagc cagcgagaag
     1261 gagctgatga tcgccggccg gctgttcatc ctggtgctga tcggcatctc catcgcctgg
     1321 gtgcctatcg tgcagtctgc ccagagcggc cagctgtttg attacatcca gtccatcacc
     1381 tcttatctgg gaccacctat cgcagccgtg ttcctgctgg ccatcttttg gaagagggtg
     1441 aacgagccag gagcattctg gggactgatc ctgggactgc tgatcggcat cagccggatg
     1501 atcaccgagt ttgcctatgg cacaggctct tgcatggagc ccagcaattg ccctacaatc
     1561 atctgtggcg tgcactacct gtatttcgcc atcatcctgt tcgccatctc ctttatcacc
     1621 atcgtggtca tctctctgct gacaaagcct atcccagacg tgcacctgta caggctgtgc
     1681 tggagcctgc gcaactccaa ggaggagcgg atcgacctgg atgccgagga ggagaatatc
     1741 caggagggcc ccaaggagac catcgagatc gagacacagg tgcctgagaa gaagaagggc
     1801 atcttccgga gagcctatga cctgttttgc ggactggagc agcacggagc accaaagatg
     1861 accgaggagg aggagaaggc catgaagatg aagatgaccg atacaagcga gaagcccctg
     1921 tggagaaccg tgctgaacgt gaatggcatc atcctggtga cagtggccgt gttctgtcac
     1981 gcctactttg cc
//
