LOCUS       sequence-253794-        1521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC7A5
FEATURES             Location/Qualifiers
     source          1..1521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1074..1085)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggccggcg ccggccctaa gaggagagcc ctggcagcac cagcagcaga ggagaaggag
       61 gaggcaaggg agaagatgct ggcagcaaag tccgccgacg gatctgcccc agcaggagag
      121 ggcgagggcg tgaccctgca gcgcaacatc acactgctga atggcgtggc catcatcgtg
      181 ggcaccatca tcggctccgg catcttcgtg accccaacag gcgtgctgaa ggaggccggc
      241 tctccaggac tggccctggt ggtgtgggca gcatgcggcg tgtttagcat cgtgggcgcc
      301 ctgtgctacg cagagctggg caccacaatc agcaagtccg gcggcgatta cgcctatatg
      361 ctggaggtgt atggcagcct gcccgccttc ctgaagctgt ggatcgagct gctgatcatc
      421 cggcctagct cccagtacat cgtggccctg gtgttcgcca cctatctgct gaagcctctg
      481 tttccaacat gcccagtgcc tgaggaggca gcaaagctgg tggcatgcct gtgcgtgctg
      541 ctgctgaccg ccgtgaactg ttactccgtg aaggccgcca caagagtgca ggacgccttc
      601 gccgcagcaa agctgctggc cctggccctg atcatcctgc tgggctttgt gcagatcggc
      661 aagggcgacg tgtctaacct ggatcctaat ttcagctttg agggcaccaa gctggatgtg
      721 ggcaacatcg tgctggccct gtactctggc ctgttcgcct acggcggctg gaactatctg
      781 aattttgtga cagaggagat gatcaacccc tataggaatc tgcctctggc catcatcatc
      841 agcctgccta tcgtgaccct ggtgtacgtg ctgacaaatc tggcctattt caccacactg
      901 tccaccgagc agatgctgtc tagcgaggcc gtggccgtgg actttggcaa ctaccacctg
      961 ggcgtgatga gctggatcat cccagtgttc gtgggcctga gctgctttgg ctccgtgaat
     1021 ggctctctgt tcacctcctc tcggctgttc tttgtgggct ctagagaggg ccacctgccc
     1081 tctatcctga gcatgatcca ccctcagctg ctgacaccag tgcccagcct ggtgttcacc
     1141 tgcgtgatga cactgctgta tgccttcagc aaggatatct tttccgtgat caacttcttt
     1201 tccttcttta attggctgtg cgtggccctg gccatcatcg gcatgatctg gctgaggcac
     1261 aggaagccag agctggagag gcccatcaag gtgaacctgg ccctgccagt gttctttatc
     1321 ctggcctgcc tgttcctgat cgccgtgtcc ttttggaaga cccccgtgga gtgtggcatc
     1381 ggcttcacaa tcatcctgtc tggcctgccc gtgtacttct tcggcgtgtg gtggaagaat
     1441 aagccaaagt ggctgctgca gggcatcttt tccaccaccg tgctgtgcca gaagctgatg
     1501 caggtggtgc cccaggagac c
//
