LOCUS       sequence-253796-        1752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC8B1
FEATURES             Location/Qualifiers
     source          1..1752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcaggcc ggagactgaa cctgagatgg gccctgtctg tgctgtgcgt gctgctgatg
       61 gcagagaccg tgagcggaac aaggggaagc tccaccggag cacacatctc tccccagttt
      121 cctgccagcg gcgtgaatca gacacccgtg gtggactgca gaaaggtgtg cggcctgaac
      181 gtgtccgaca ggtgcgattt catccgcacc aatcctgact gtcactctga tggcggctac
      241 ctggattatc tggagggcat cttctgccac tttccaccta gcctgctgcc tctggcagtg
      301 accctgtacg tgtcctggct gctgtatctg tttctgatcc tgggcgtgac agccgccaag
      361 ttcttttgtc caaacctgtc cgccatctct accacactga agctgagcca caacgtggcc
      421 ggcgtgacct tcctggcctt tggcaatggc gccccagaca tcttctccgc cctggtggcc
      481 ttttctgatc cacacacagc aggactggcc ctgggcgccc tgttcggagc aggcgtgctg
      541 gtgaccacag tggtggcagg aggaatcacc atcctgcacc ctttcatggc agcatccagg
      601 ccattcttta gagacatcgt gttttacatg gtggccgtgt tcctgacctt tctgatgctg
      661 ttcaggggaa gggtgacact ggcatgggca ctgggatacc tgggactgta cgtgttttat
      721 gtggtgaccg tgatcctgtg cacatggatc tatcagaggc agaggcgcgg ctctctgttc
      781 tgtcctatgc cagtgacccc cgagatcctg tccgactctg aggaggatcg ggtgtctagc
      841 aacacaaata gctacgacta tggcgatgag tacagacctc tgttctttta tcaggagacc
      901 acagcccaga tcctggtgag ggccctgaat ccactggact acatgaagtg gcggagaaag
      961 agcgcctatt ggaaggccct gaaggtgttc aagctgcccg tggagtttct gctgctgctg
     1021 accgtcccag tggtggaccc cgataaggac gatcagaact ggaagcgccc cctgaattgt
     1081 ctgcacctgg tcatctcccc tctggtcgtg gtgctgaccc tgcagtctgg cacatacggc
     1141 gtgtatgaga tcggaggact ggtgcccgtg tgggtggtgg tggtcatcgc aggaacagcc
     1201 ctggcatccg tgaccttctt tgccacaagc gattcccagc caccaaggct gcactggctg
     1261 ttcgcctttc tgggctttct gacctctgcc ctgtggatca acgccgccgc cacagaggtg
     1321 gtgaatatcc tgaggtctct gggcgtggtg ttccgcctga gcaacaccgt gctgggactg
     1381 acactgctgg catggggcaa tagcatcggc gacgccttct ccgacttcac cctggcaagg
     1441 cagggatacc caagaatggc cttcagcgcc tgctttggcg gcatcatctt caacatcctg
     1501 gtgggagtgg gactgggatg tctgctgcag atctctagaa gccacacaga ggtgaagctg
     1561 gagccagatg gcctgctggt gtgggtgctg gcaggcgccc tgggactgag cctggtgttt
     1621 agcctggtgt ccgtgcccct gcagtgcttc cagctgtcca gggtgtacgg cttttgtctg
     1681 ctgctgttct atctgaattt tctggtggtg gccctgctga ccgagttcgg cgtgatccac
     1741 ctgaagtcca tg
//
