LOCUS       sequence-253802-        2055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC3A1
FEATURES             Location/Qualifiers
     source          1..2055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             207..215
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggccgagg ataagtctaa gagggacagc atcgagatgt ccatgaaggg ctgccagacc
       61 aacaatggct tcgtgcacaa cgaggatatc ctggagcaga caccagatcc cggcagctcc
      121 accgacaatc tgaagcacag cacaagggga atcctgggat cccaggagcc agacttcaag
      181 ggagtgcagc catacgcagg aatgcctaag gaggtgctgt tccagttttc cggccaggcc
      241 cggtatagaa tccccagaga gatcctgttt tggctgaccg tggcatccgt gctggtgctg
      301 atcgcagcaa caatcgccat catcgccctg tctcctaagt gtctggactg gtggcaggag
      361 ggccctatgt accagatcta tccacgctct ttcaaggaca gcaacaagga tggcaatggc
      421 gacctgaagg gcatccagga caagctggat tacatcaccg ccctgaacat caagacagtg
      481 tggatcacca gcttttacaa gtctagcctg aaggatttca ggtacggcgt ggaggatttt
      541 cgcgaggtgg acccaatctt cggcaccatg gaggactttg agaacctggt ggccgccatc
      601 cacgataagg gcctgaagct gatcatcgac ttcatcccca atcacacctc tgataagcac
      661 atctggtttc agctgagcag gacacgcacc ggcaagtaca cagattacta tatctggcac
      721 gactgcacac acgagaacgg caagaccatc ccccctaaca attggctgtc cgtgtacggc
      781 aactcctctt ggcacttcga cgaggtgcgg aatcagtgtt atttccacca gtttatgaag
      841 gagcagcccg atctgaactt ccggaatcct gacgtgcagg aggagatcaa ggagatcctg
      901 agattttggc tgaccaaggg cgtggatggc ttctccctgg acgccgtgaa gtttctgctg
      961 gaggccaagc acctgagaga tgagatccaa gtgaataaga cacagatccc tgacacagtg
     1021 acccagtact ctgagctgta tcacgacttc accacaaccc aagtgggcat gcacgatatc
     1081 gtgaggtcct ttcgccagac aatggaccag tactctaccg agccaggccg gtatagattc
     1141 atgggcaccg aggcctacgc cgagtctatc gataggacag tgatgtacta tggcctgccc
     1201 ttcatccagg aggccgattt cccctttaac aattacctgt ctatgctgga caccgtgtct
     1261 ggcaacagcg tgtatgaagt gatcacaagc tggatggaga acatgccaga gggcaagtgg
     1321 cccaattgga tgatcggagg accagacagc tccaggctga ccagcagact gggcaatcag
     1381 tacgtgaacg tgatgaatat gctgctgttc acactgcctg gcacaccaat cacctactat
     1441 ggcgaggaga tcggcatggg caacatcgtg gccgccaacc tgaatgagag ctatgatatc
     1501 aataccctga ggagcaagtc ccctatgcag tgggacaact ctagcaatgc cggcttttcc
     1561 gaggcctcta acacatggct gcctaccaat tccgattacc acaccgtgaa cgtggacgtg
     1621 cagaagacac agccacgcag cgccctgaag ctgtatcagg atctgtccct gctgcacgcc
     1681 aacgagctgc tgctgaatcg gggctggttc tgccacctga gaaacgacag ccactacgtg
     1741 gtgtatacca gggagctgga cggcatcgat cgcatcttca tcgtggtgct gaattttggc
     1801 gagtctacac tgctgaacct gcacaatatg atcagcggac tgccagcaaa gatgaggatc
     1861 aggctgagca ccaactccgc cgataagggc tccaaggtgg acacatctgg catcttcctg
     1921 gataagggcg agggcctgat ctttgagcac aacaccaaga atctgctgca caggcagaca
     1981 gccttccggg acagatgctt tgtgagcaac cgcgcctgtt actcctctgt gctgaatatc
     2041 ctgtatacct cctgt
//
