LOCUS       sequence-253804-        1473 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC43A3
FEATURES             Location/Qualifiers
     source          1..1473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1315..1326)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggcaggac agggactgcc actgcacgtg gccaccctgc tgacaggact gctggagtgc
       61 ctgggattcg caggcgtgct gtttggatgg ccatccctgg tgttcgtgtt taagaacgag
      121 gactacttca aggatctgtg cggaccagac gcaggaccta tcggaaatgc aaccggacag
      181 gcagactgta aggcacagga tgagcggttc tctctgatct ttacactggg cagctttatg
      241 aacaatttca tgacctttcc cacaggctac atcttcgatc ggtttaagac cacagtggcc
      301 agactgatcg ccatcttctt ttataccaca gccaccctga tcatcgcctt cacaagcgcc
      361 ggatccgccg tgctgctgtt tctggccatg cctatgctga ccatcggcgg catcctgttc
      421 ctgatcacaa acctgcagat cggcaatctg tttggtcaac atagatccac catcataacc
      481 ctgtacaacg gcgccttcga cagctcctct gccgtgtttc tgatcatcaa gctgctgtat
      541 gagaagggca tcagcctgag agcctccttc atctttatca gcgtgtgcag cacctggcac
      601 gtggccagga cattcctgct gatgccaagg ggacacatcc catacccact gccccctaac
      661 tacagctatg gcctgtgccc tggcaatggc accacaaagg aggagaagga gaccgccgag
      721 cacgagaata gggagctgca gagcaaggag ttcctgtccg ccaaggagga gacaccagga
      781 gcaggacaga agcaggagct gcgctccttt tggtcttacg ccttctcccg gagatttgcc
      841 tggcacctgg tgtggctgtc tgtgatccag ctgtggcact atctgttcat cggcaccctg
      901 aactccctgc tgacaaatat ggcaggaggc gacatggcaa gggtgtctac ctacacaaac
      961 gccttcgcct ttacccagtt tggcgtgctg tgcgcccctt ggaatggcct gctgatggat
     1021 cggctgaagc agaagtatca gaaggaggcc agaaagaccg gaagctccac actggcagtg
     1081 gccctgtgct ctaccgtgcc aagcctggcc ctgacaagcc tgctgtgcct gggattcgca
     1141 ctgtgcgcat ccgtgcctat cctgccactg cagtacctga ccttcatcct gcaggtcatc
     1201 tctcgcagct ttctgtatgg ctctaacgcc gccttcctga ccctggcctt tcctagcgag
     1261 cacttcggca agctgtttgg actggtcatg gcactgtccg ccgtggtgtc tctgctgcag
     1321 ttcccaatct ttaccctgat caagggcagc ctgcagaacg atcccttcta cgtgaatgtg
     1381 atgtttatgc tggccatcct gctgaccttc tttcaccctt tcctggtgta tagggagtgt
     1441 cgcacatgga aggagtcccc atctgccatc gcc
//
