LOCUS       sequence-253820-        2445 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC9A1
FEATURES             Location/Qualifiers
     source          1..2445
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1213..1227
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 atggtgctgc gctctggaat ctgcggactg agcccacacc ggatcttccc ctccctgctg
       61 gtggtggtgg ccctggtggg actgctgcct gtgctgagat ctcacggact gcagctgagc
      121 ccaaccgcat ccacaatcag gagctccgag ccacctaggg agcggagcat cggcgacgtg
      181 accacagcac caccagaggt gacacctgag tccaggccag tgaaccactc tgtgaccgat
      241 cacggcatga agcctcggaa ggccttccca gtgctgggca tcgactacac ccacgtgaga
      301 acaccctttg agatcagcct gtggatcctg ctggcctgcc tgatgaagat cggcttccac
      361 gtgatcccca caatctctag catcgtgcct gagtcctgtc tgctgatcgt ggtgggactg
      421 ctggtgggag gactgatcaa gggagtggga gagacccctc cattcctgca gtctgacgtg
      481 ttcttcctgt tcctgctgcc ccctatcatc ctggacgccg gctacttcct gccactgagg
      541 cagtttaccg agaacctggg cacaatcctg atcttcgccg tggtgggcac actgtggaat
      601 gccttctttc tgggcggcct gatgtatgcc gtgtgcctgg tgggaggaga gcagatcaac
      661 aatatcggcc tgctggataa cctgctgttt ggcagcatca tctccgccgt ggacccagtg
      721 gccgtgctgg ccgtgttcga ggagatccac atcaacgagc tgctgcacat cctggtgttt
      781 ggcgagtctc tgctgaatga tgccgtgacc gtggtgctgt accacctgtt cgaggagttt
      841 gccaattatg agcacgtggg catcgtggac atcttcctgg gctttctgag cttctttgtg
      901 gtggccctgg gaggcgtgct ggtgggagtg gtgtacggcg tgatcgcagc cttcacctct
      961 cggttcacca gccacatcag agtgatcgag cccctgttcg tgtttctgta cagctatatg
     1021 gcctatctgt ccgccgagct gttccacctg tctggcatca tggcactgat cgcaagcgga
     1081 gtggtcatgc ggccttacgt ggaggccaat atctctcaca agagccacac cacaatcaag
     1141 tattttctga agatgtggtc ctctgtgagc gagacactga tcttcatctt tctgggcgtg
     1201 agcaccgtgg caggatccca ccactggaac tggaccttcg tgatctccac actgctgttt
     1261 tgcctgatcg caagggtgct gggcgtgctg ggactgacat ggttcatcaa taagtttcgc
     1321 atcgtgaagc tgacccccaa ggatcagttc atcatcgcat acggaggact gagaggagca
     1381 atcgcctttt ccctgggcta tctgctggat aagaagcact tccctatgtg cgacctgttt
     1441 ctgaccgcca tcatcacagt gatcttcttt acagtgttcg tgcagggcat gaccatcagg
     1501 ccactggtgg atctgctggc cgtgaagaag aagcaggaga ccaagcgcag catcaacgag
     1561 gagatccaca cacagtttct ggatcacctg ctgaccggca tcgaggacat ctgcggccac
     1621 tacggccacc accactggaa ggacaagctg aaccggttca ataagaagta tgtgaagaag
     1681 tgtctgatcg ccggcgagag atctaaggag cctcagctga tcgcctttta ccacaagatg
     1741 gagatgaagc aggcaatcga gctggtggag agcggaggaa tgggcaagat cccatccgcc
     1801 gtgtctaccg tgagcatgca gaatatccac cccaagtccc tgccttctga gaggatcctg
     1861 cccgccctgt ccaaggataa ggaggaggag atccgcaaga tcctgcggaa caatctgcag
     1921 aagacacggc agagactgag gtcttacaac aggcacaccc tggtggccga cccatatgag
     1981 gaggcctgga atcagatgct gctgcggaga cagaaggccc gccagctgga gcagaagatc
     2041 aacaattacc tgacagtgcc agcccacaag ctggattccc caaccatgtc tagggcaagg
     2101 atcggatccg acccactggc atatgagcct aaggaggatc tgccagtgat caccatcgac
     2161 ccagcaagcc cacagtcccc tgagtctgtg gacctggtga acgaggagct gaagggcaag
     2221 gtgctgggac tgagcaggga tcctgcaaag gtggcagagg aggacgagga cgatgacgga
     2281 ggcatcatga tgagatccaa ggagaccagc tcccccggca cagatgacgt gttcacccca
     2341 gccccctccg attctccttc tagccagaga atccagaggt gcctgagcga ccctggacca
     2401 cacccagagc ctggagaggg agagccattc tttcccaagg gccag
//
