LOCUS       sequence-25385-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC180
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(147..452)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(249..268)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tgacgagaaa cgtaaaatga tataaatatc aatatattaa attagatnnn nnnnntaaaa
       61 aacagactac ataatactgt aaaacacaac atatccagtc actatggtcg acctgcagac
      121 tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt aatggcgttt
      181 ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac ggagaccggc
      241 acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg
      301 taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg gatcaccatc
      361 cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc
      421 tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcagcaaa
      481 agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt
      541 ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga
      601 natattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta
      661 atacgctgct tcatagcata cctctttttg acatacttcg gntatacata tcagtatata
      721 ttcntatacc gcaaaaatca gcgcgcaaan tacgcatact gttatctg
//
