LOCUS       sequence-253900-        1476 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SVOPL
FEATURES             Location/Qualifiers
     source          1..1476
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1328..1337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggccacca agcccacaga gcctgtgacc atcctgtctc tgaggaagct gagcctgggc
       61 acagccgagc cacaggtgaa ggagcccaag acctttacag tggaggacgc agtggagacc
      121 atcggattcg gccgctttca catcgccctg ttcctgatca tgggctccac aggcgtggtg
      181 gaggccatgg agatcatgct gatcgccgtg gtgtctccag tgatcaggtg cgagtggcag
      241 ctggagaact ggcaggtggc cctggtgacc acaatggtgt tctttggcta catggtgttc
      301 agcatcctgt ttggcctgct ggccgatcgg tatggcagat ggaagatcct gctgatctcc
      361 ttcctgtggg gcgcctactt ttctctgctg accagcttcg ccccctccta tatctggttc
      421 gtgtttctga ggacaatggt gggatgtggc gtgagcggac actcccaggg actgatcatc
      481 aagaccgagt ttctgccaac aaagtaccgc ggctatatgc tgccactgag ccaggtgttc
      541 tggctggcag gatccctgct gatcatcgga ctggcctctg tgatcatccc gactatagga
      601 tggcgatggt tgatcagggt ggcatccatc cctggcatca tcctgatcgt ggccttcaag
      661 tttatcccag agtccgccag gttcaacgtg tctaccggaa atacaagggc cgccctggcc
      721 accctggaga gggtggccaa gatgaataga agcgtgatgc ctgagggcaa gctggtggag
      781 ccagtgctgg agaagagggg ccgctttgcc gacctgctgg atgccaagta cctgcggacc
      841 acactgcaga tctgggtcat ctggctgggc atctctttcg cctactatgg cgtgatcctg
      901 gcaagcgccg agctgctgga gagagacctg gtgtgcggct ccaagtctga tagcgccgtg
      961 gtggtgaccg gaggcgactc cggcgagtcc cagtctccct gctactgtca catgtttgcc
     1021 cctagcgatt atcggaccat gatcatctcc acaatcggcg agatcgccct gaacccactg
     1081 aatatcctgg gcatcaactt cctgggccgg agactgagcc tgtccatcac catgggctgc
     1141 acagccctgt tctttctgct gctgaatatc tgtaccagct ccgccggcct gatcggcttc
     1201 ctgtttatgc tgagagccct ggtggccgcc aactttaata ccgtgtacat ctatacagcc
     1261 gaggtgtatc caaccacaat gcgggccctg ggaatgggca catctggaag cctgtgcaga
     1321 attggcgcca tggtggcccc tttcatctct caggtgctga tgtccgcctc tatcctgggc
     1381 gccctgtgcc tgttttctag cgtgtgcgtg gtgtgcgcca tcagcgcctt caccctgcca
     1441 atcgagacaa agggacgggc cctgcagcag atcaag
//
