LOCUS       sequence-253920-        3237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC4A4
FEATURES             Location/Qualifiers
     source          1..3237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaggacg aggccgtgct ggatagggga gcaagcttcc tgaagcacgt gtgcgacgag
       61 gaggaggtgg agggccacca caccatctac atcggcgtgc acgtgcccaa gagctatcgg
      121 agaaggcgcc ggcacaagag aaagacaggc cacaaggaga agaaggagaa ggagaggatc
      181 tccgagaact actccgacaa gtctgatatc gagaatgccg acgagagctc ctctagcatc
      241 ctgaagcccc tgatctctcc tgcagcagag aggatccggt tcatcctggg agaggaggac
      301 gatagcccag caccacctca gctgtttacc gagctggacg agctgctggc cgtggatggc
      361 caggagatgg agtggaagga gacagcccgc tggatcaagt tcgaggagaa ggtggagcag
      421 ggaggagagc ggtggtctaa gcctcacgtg gccaccctga gcctgcactc cctgtttgag
      481 ctgcgcacat gtatggagaa gggcagcatc atgctggacc gggaggcctc ctctctgcct
      541 cagctggtgg agatgatcgt ggaccaccag atcgagaccg gcctgctgaa gccagagctg
      601 aaggataagg tgacctatac actgctgcgc aagcaccggc accagacaaa gaagtccaac
      661 ctgagatctc tggccgacat cggcaagacc gtgagctccg cctctaggat gttcacaaac
      721 cccgataatg gcagccctgc catgacccac cgcaacctga catctagctc cctgaatgac
      781 atctccgata agccagagaa ggaccagctg aagaacaagt tcatgaagaa gctgccccgg
      841 gatgccgagg cctctaatgt gctggtgggc gaggtggact ttctggatac cccattcatc
      901 gcctttgtga gactgcagca ggccgtgatg ctgggcgccc tgaccgaggt gcctgtgcca
      961 acaaggttcc tgtttatcct gctgggcccc aagggcaagg ccaagtccta ccacgagatc
     1021 ggcagagcca tcgccaccct gatgtctgac gaggtgttcc acgatatcgc ctataaggcc
     1081 aaggacaggc acgatctgat cgccggcatc gacgagtttc tggatgaagt gatcgtgctg
     1141 ccaccaggag agtgggaccc agccatcaga atcgagcctc caaagagcct gccttctagc
     1201 gataagagga agaacatgta ctccggcggc gagaacgtgc agatgaatgg cgacacccct
     1261 cacgatggag gacacggagg aggaggacac ggcgactgcg aggagctgca gagaacaggc
     1321 aggttctgtg gcggcctgat caaggatatc aagaggaagg ccccattctt tgcctctgac
     1381 ttttacgatg ccctgaatat ccaggccctg agcgccatcc tgttcatcta tctggccacc
     1441 gtgacaaacg ccatcacctt tggcggactg ctgggcgacg caacagataa tatgcagggc
     1501 gtgctggagt ccttcctggg aaccgccgtg tctggagcca tcttctgcct gtttgccggc
     1561 cagccactga ccatcctgtc ctctacaggc cccgtgctgg tgtttgagcg cctgctgttc
     1621 aacttcagca aggacaacaa tttcgattac ctggagtttc ggctgtggat cggcctgtgg
     1681 agcgccttcc tgtgcctgat cctggtggcc accgacgcct cctttctggt gcagtacttc
     1741 acccgcttta cagaggaggg cttcagctcc ctgatctcct tcatctttat ctatgacgcc
     1801 tttaagaaga tgatcaagct ggccgattac tatcccatca actccaattt caaggtcggc
     1861 tacaacaccc tgttttcttg cacatgcgtg ccccctgacc ctgccaacat cagcatctcc
     1921 aatgatacca cactggcccc agagtacctg cccaccatgt ctagcacaga catgtatcac
     1981 aataccacat tcgattgggc ctttctgagc aagaaggagt gctccaagta tggcggcaac
     2041 ctggtgggca acaattgtaa tttcgtgccc gacatcaccc tgatgtcctt catcctgttt
     2101 ctgggcacct acacatcctc tatggccctg aagaagttca agacatctcc atattttccc
     2161 accacagcca gaaagctgat cagcgatttc gccatcatcc tgtccatcct gatcttttgc
     2221 gtgatcgacg ccctggtggg cgtggatacc ccaaagctga tcgtgcccag cgagttcaag
     2281 cctacatccc caaacagggg ctggttcgtg ccaccctttg gcgagaatcc ttggtgggtg
     2341 tgcctggcag cagcaatccc agccctgctg gtgaccatcc tgatcttcat ggaccagcag
     2401 atcacagccg tgatcgtgaa ccgcaaggag cacaagctga agaagggcgc cggctaccac
     2461 ctggatctgt tttgggtggc catcctgatg gtcatctgca gcctgatggc actgccatgg
     2521 tacgtggcag caaccgtgat ctctatcgcc cacatcgaca gcctgaagat ggagaccgag
     2581 acatccgccc ctggagagca gccaaagttc ctgggcgtgc gcgagcagag ggtgaccggc
     2641 acactggtgt ttatcctgac cggcctgagc gtgttcatgg ccccaatcct gaagtttatc
     2701 cccatgcctg tgctgtacgg cgtgttcctg tatatgggcg tggcctccct gaatggcgtg
     2761 cagtttatgg accgcctgaa gctgctgctg atgcctctga agcaccagcc agatttcatc
     2821 tacctgcggc acgtgcctct gagaagggtg cacctgttca cctttctgca ggtgctgtgc
     2881 ctggccctgc tgtggatcct gaagagcaca gtggccgcca tcatcttccc agtgatgatc
     2941 ctggccctgg tggccgtgcg gaagggcatg gactacctgt tctctcagca cgatctgagc
     3001 tttctggacg atgtgatccc cgagaaggac aagaagaaga aggaggatga gaagaagaag
     3061 aagaagaaga agggctctct ggacagcgat aacgacgatt ccgactgccc ttattctgag
     3121 aaggtgccca gcatcaagat ccctatggat atcatggagc agcagccatt cctgtctgac
     3181 agcaagccct ccgatagaga gaggtctcct acctttctgg agagacacac atcctgt
//
