LOCUS       sequence-253924-        1488 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_TMEM104
FEATURES             Location/Qualifiers
     source          1..1488
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccggcg agatcacaga gaccggcgag ctgtacagct cctatgtggg cctggtgtac
       61 atgtttaacc tgatcgtggg aacaggcgcc ctgaccatgc caaaggcctt cgccaccgca
      121 ggatggctgg tgtccctggt gctgctggtg ttcctgggct ttatgtcttt cgtgaccaca
      181 acctttgtga tcgaggcaat ggcagcagca aacgcgcagt tgcattggaa acgcatggag
      241 aatctgaagg aggaggagga cgatgactct agcaccgcca gcgattccga cgtgctgatc
      301 agagataact acgagagggc cgagaagcgc cctatcctga gcgtgcagcg gagaggctcc
      361 cctaatccat ttgagatcac cgaccgggtg gagatgggcc agatggccag catgttcttt
      421 aacaaggtcg gcgtgaatct gttctacttt tgcatcatcg tgtacctgta tggcgatctg
      481 gccatctatg ccgccgccgt gccattctcc ctgatgcagg tgacatgctc tgccaccggc
      541 aacgatagct gtggcgtgga ggccgacaca aagtacaatg ataccgacag gtgttgggga
      601 ccactgagga gggtggatgc atacagaatc tatctggcca tcttcacact gctgctgggc
      661 ccttttacct tctttgacgt gcagaagaca aagtatctgc agatcctgac cagcctgatg
      721 aggtggatcg ccttcgccgt gatgatcgtg ctggccctga tccgcatcgg acacggacag
      781 ggagagggac acccacctct ggcagacttc tctggcgtga ggaatctgtt tggcgtgtgc
      841 gtgtacagct tcatgtgcca gcactctctg cccagcctga tcacacccgt gagctctaag
      901 cggcacctga ccagactggt gtttctggat tacgtgctga tcctggcctt ctatggcctg
      961 ctgagcttta cagccatctt ctgctttcgc ggcgattccc tgatggacat gtataccctg
     1021 aacttcgcaa ggtgtgacgt ggtgggactg gcagccgtgc gcttctttct gggcctgttt
     1081 ccagtgttca caatctctac caattttccc atcatcgccg tgacactgcg gaacaattgg
     1141 aagaccctgt tccacagaga gggcggcaca tacccttggg tggtggatag agtggtgttt
     1201 ccaacaatca ccctggtgcc acccgtgctg gtggccttct gcacccacga cctggagtcc
     1261 ctggtgggaa tcacaggagc atacgcagga accggaatcc agtatgtgat cccagccttt
     1321 ctggtgtatc actgccggag agatacacag ctggccttcg gctgtggcgt gtccaacaag
     1381 cacaggtctc cctttcgcca caccttctgg gtgggctttg tgctgctgtg ggccttctcc
     1441 tgtttcatct ttgtgacagc caatatcatc ctgtctgaga ccaagctg
//
