LOCUS       sequence-25394-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Nausch LW, Ledoux J, Bonev AD, Nelson MT, Dostmann WR
  TITLE     Differential patterning of cGMP in vascular smooth muscle cells 
            revealed by single GFP-linked biosensors.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):365-70.
  PUBMED    18165313
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Jan 8. 105(1):365-70."
COMMENT     Sequence Label: pRSETA-alphaFlincG
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..99
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(103..111,112..126)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 aatattttgt ttntttaaga aggagatata catatgcggg gttctcatca tcatcatcat
       61 catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac
      121 gataaggatc gatggggatc cgagctcgag gcgatgagcg agctggagga agactttgcc
      181 aagattctca tgctcaagga ggagaggatc aaagagctgg agaagcggct gtcagagaag
      241 gaggaagaaa tccaggagct gaagaggaaa ctccataaat gccagtcagt gctgcccgtg
      301 ccctcgaccc acatcggccc ccggaccacc cgggcacagg gcatctcggc cgagccgcag
      361 acctacaggt ccttccacga cctccgacag gcattccgga agttcaccaa atccgaaagg
      421 tccaaggatc tcataaagga ggccatcctt gacaatgact ttatgaagaa cttggagctg
      481 tcacagatcc aagagattgt ggattgtatg tacccagtgg agtacggcaa agacagctgc
      541 atcatcaaag aaggagatgt ggggtcactg gtgtatgtca tggaagatgg taaggttgaa
      601 gttacaaaag aaggcgtgaa gctgtgcaca atgggtcctg gtaaagtgtt tggagagttg
      661 gctatccttt acaactgtac ccggacggcg accgtcaaaa
//
