LOCUS       sequence-253950-        2493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Polesel M, Ingles-Prieto A, Christodoulaki E, Ferrada E, Doucerain 
            C, Altermatt P, Knecht M, Kuhn M, Steck AL, Wilhelm M, Manolova V
  TITLE     Functional characterization of SLC39 family members ZIP5 and ZIP10 
            in overexpressing HEK293 cells reveals selective copper transport 
            activity.
  JOURNAL   Biometals. 2022 Dec 1. doi: 10.1007/s10534-022-00474-6.
  PUBMED    36454509
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biometals. 
            2022 Dec 1. doi: 10.1007/s10534-022-00474-6."
COMMENT     Sequence Label: pDONR221_SLC39A10
FEATURES             Location/Qualifiers
     source          1..2493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaaggtgc acatgcacac caagttctgc ctgatctgtc tgctgacatt catctttcac
       61 cactgcaacc actgtcacga ggagcacgat cacggaccag aggccctgca caggcagcac
      121 agaggcatga ccgagctgga gccctctaag ttcagcaagc aggccgccga gaacgagaag
      181 aagtactata tcgagaagct gtttgagcgg tacggcgaga atggcagact gagcttcttt
      241 ggcctggaga agctgctgac caacctgggc ctgggcgaga ggaaggtggt ggagatcaat
      301 cacgaggatc tgggccacga ccacgtgtcc cacctggaca tcctggccgt gcaggagggc
      361 aagcactttc actcccacaa ccaccagcac tctcacaacc acctgaatag cgagaaccag
      421 accgtgacaa gcgtgtccac caagcgcaat cacaagtgcg atcccgagaa ggagacagtg
      481 gaggtgtccg tgaagtctga cgataagcac atgcacgacc acaaccaccg gctgagacac
      541 caccacaggc tgcaccacca cctggatcac aacaataccc accacttcca caatgactct
      601 atcacaccta gcgagcgcgg cgagccttcc aatgagccat ctaccgagac aaacaagacc
      661 caggagcagt ccgatgtgaa gctgccaaag ggcaagcgga agaagaaggg ccgcaagagc
      721 aacgagaatt ccgaagtgat cacacccggc tttcccccta atcacgatca gggcgagcag
      781 tatgagcaca acagggtgca caagcccgac cgcgtgcaca atcctggaca ctcccacgtg
      841 cacctgccag agaggaacgg acacgaccca ggaagaggac accaggacct ggatcctgac
      901 aatgagggcg agctgaggca caccaggaag agggaggcac cacacgtgaa gaacaatgcc
      961 atcatcagcc tgagaaagga tctgaacgag gacgatcacc accacgagtg tctgaatgtg
     1021 acacagctgc tgaagtacta tggacacgga gcaaacagcc caatctccac cgacctgttc
     1081 acatacctgt gccctgccct gctgtatcag atcgattccc ggctgtgcat cgagcacttt
     1141 gacaagctgc tggtggagga tatcaataag gacaagaacc tggtgccaga ggacgaggca
     1201 aacatcggag catctgcctg gatctgcggc atcatcagca tcaccgtgat ctctctgctg
     1261 agcctgctgg gcgtgatcct ggtgcctatc atcaatcagg gctgtttcaa gtttctgctg
     1321 accttcctgg tggccctggc agtgggaaca atgagcggcg atgcactgct gcacctgctg
     1381 ccacactccc agggaggaca tgaccactct caccagcacg cacacggaca cggacactct
     1441 cacggacacg agagcaacaa gttcctggag gagtacgatg ccgtgctgaa gggactggtg
     1501 gccctgggag gcatctatct gctgttcatc atcgagcact gcatcaggat gtttaagcac
     1561 tacaagcagc agcgcggcaa gcagaagtgg tttatgaagc agaataccga ggagagcaca
     1621 atcggccgga agctgtccga ccacaagctg aacaataccc ctgattctga ctggctgcag
     1681 ctgaagccac tggccggcac agacgattcc gtggtgtctg aggatagact gaacgagacc
     1741 gagctgacag acctggaggg acagcaggag agcccaccaa agaattacct gtgcatcgag
     1801 gaggagaaga tcatcgatca ctctcacagc gacggcctgc acaccatcca cgagcacgat
     1861 ctgcacgcag cagcacacaa tcaccacgga gagaacaaga cagtgctgag aaagcacaac
     1921 caccagtggc accacaagca cagccaccac tcccacggac catgccactc cggatctgac
     1981 ctgaaggaga ccggcatcgc caacatcgcc tggatggtca tcatgggcga tggcatccac
     2041 aatttctccg acggactggc aatcggagca gccttttctg ccggactgac cggaggaatc
     2101 agcacatcca tcgccgtgtt ctgtcacgag ctgcctcacg agctgggcga ttttgccgtg
     2161 ctgctgaagg ccggcatgac cgtgaagcag gccatcgtgt acaatctgct gagcgccatg
     2221 atggcctata tcggcatgct gatcggcaca gccgtgggcc agtacgccaa caatatcacc
     2281 ctgtggatct tcgccgtgac agccggcatg tttctgtatg tggccctggt ggacatgctg
     2341 cccgagatgc tgcacggcga tggcgacaac gaggagcacg gcttctgccc tgtgggccag
     2401 tttatcctgc agaatctggg cctgctgttc ggctttgcca tcatgctggt catcgccctg
     2461 tacgaggata agatcgtgtt cgacatccag ttt
//
