LOCUS       sequence-253952-        3723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC4A2
FEATURES             Location/Qualifiers
     source          1..3723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagctccg ccccacggag acccgcaaag ggagcagatt ctttctgcac accagagcca
       61 gagagcctgg gacctggaac cccaggattt ccagaacagg aggaggacga gctgcaccgc
      121 accctgggag tggagcggtt cgaggagatc ctgcaggagg caggaagcag aggaggagag
      181 gagccaggca ggtcttacgg agaggaggat tttgagtatc acagacagtc tagccaccac
      241 atccaccacc cactgagcac acacctgcca cctgacgcaa ggcgccggaa gacccctcag
      301 ggcccaggaa ggaagccaag aaggcgcccc ggagcatctc ctaccggaga gaccccaaca
      361 atcgaggagg gagaggagga cgaggatgag gcaagcgagg cagagggagc aagagccctg
      421 acccagcctt ccccagtgtc tacaccttcc tctgtgcagt tctttctgca ggaggacgat
      481 agcgccgaca gaaaggccga gaggacatcc ccaagctccc cagcacctct gccacaccag
      541 gaggccacac ctagggccag caagggcgcc caggccggca cccaggtgga ggaggccgag
      601 gccgaggcag tggcagtggc aagcggaacc gcaggcggcg acgatggcgg cgcctccggc
      661 agacccctgc ctaaggcaca gcctggacac aggtcttaca acctgcagga gcggagaagg
      721 atcggaagca tgacaggagc agagcaggcc ctgctgccaa gagtgcctac cgatgagatc
      781 gaggcacaga ccctggcaac agcagacctg gatctgatga agtcccacag attcgaggac
      841 gtgccaggcg tgcgccggca cctggtgagg aagaatgcca agggcagcac acagtccggc
      901 agagagggca gggagccagg accaacccct agggcaaggc caagggcacc ccacaagcct
      961 cacgaggtgt tcgtggagct gaacgagctg ctgctggata agaatcagga gcctcagtgg
     1021 agggagacag caaggtggat caagttcgag gaggacgtgg aggaggagac cgagcggtgg
     1081 ggcaagccac acgtggcctc tctgagcttt cggagcctgc tggagctgag aaggacactg
     1141 gcacacggag ccgtgctgct ggacctggat cagcagaccc tgccaggagt ggcacaccag
     1201 gtggtggagc agatggtcat ctccgatcag atcaaggcag aggacagggc aaacgtgctg
     1261 cgggccctgc tgctgaagca ctctcacccc agcgacgaga aggatttctc ttttcctcgg
     1321 aatatctccg ccggatctct gggaagcctg ctgggacacc accacggaca gggagccgag
     1381 agcgatccac acgtgacaga gccactgatg ggaggagtgc ctgagacccg cctggaggtg
     1441 gagagagaga gggagctgcc accacctgca ccaccagcag gcatcacccg gtccaagtct
     1501 aagcacgagc tgaagctgct ggagaagatc cccgagaacg cagaggcaac agtggtgctg
     1561 gtgggatgcg tggagttcct gagcaggcct accatggcct ttgtgcgcct gagggaggca
     1621 gtggagctgg atgccgtgct ggaggtgcca gtgcccgtga gattcctgtt tctgctgctg
     1681 ggcccctcta gcgccaacat ggattaccac gagatcggca ggagcatctc cacactgatg
     1741 tccgacaagc agttccacga ggccgcctat ctggcagatg agagggagga cctgctgacc
     1801 gccatcaatg cctttctgga ctgttccgtg gtgctgcctc catctgaggt gcagggagag
     1861 gagctgctgc gcagcgtggc acacttccag cggcagatgc tgaagaagag agaggagcag
     1921 ggcaggctgc tgcctacagg agcaggactg gagccaaagt ccgcccagga taaggccctg
     1981 ctgcagatgg tggaggcagc aggagcagca gaggacgatc cactgaggag gaccggcaga
     2041 cctttcggcg gcctgatcag ggacgtgaga aggcgctacc ctcactatct gagcgacttt
     2101 agagatgcac tggaccctca gtgcctggca gccgtgatct tcatctactt tgccgccctg
     2161 tccccagcca tcacattcgg cggactgctg ggagagaaga cccaggacct gatcggcgtg
     2221 agcgagctga tcatgtccac cgccctgcag ggcgtggtgt tttgcctgct gggagcacag
     2281 ccactgctgg tcatcggctt tagcggacca ctgctggtgt tcgaggaggc cttcttttcc
     2341 ttttgttcct ctaatcacct ggagtatctg gtgggcaggg tgtggatcgg cttctggctg
     2401 gtgtttctgg ccctgctgat ggtggccctg gagggaagct tcctggtgag attcgtgagc
     2461 aggttcacac aggagatctt cgcctttctg atcagcctga tctttatcta cgagaccttc
     2521 tataagctgg tgaagatctt ccaggagcac cctctgcacg gctgttctgc cagcaacagc
     2581 tccgaggtgg atggaggaga gaatatgacc tgggcaggag caaggccaac actgggacca
     2641 ggaaaccggt ctctggcagg acagagcgga cagggcaagc ctagaggaca gccaaataca
     2701 gccctgctgt ccctggtgct gatggcaggc accttcttta tcgccttctt tctgaggaag
     2761 ttcaagaact ctcgcttctt tccaggccgg atccggagag tgatcggcga ctttggcgtg
     2821 cccatcgcca tcctgatcat ggtgctggtg gattactcca tcgaggacac ctatacacag
     2881 aagctgtccg tgccatctgg cttcagcgtg acagcaccag agaagagggg atgggtcatc
     2941 aaccctctgg gcgagaagtc tccttttcca gtgtggatga tggtggccag cctgctgcca
     3001 gccatcctgg tgtttatcct gatcttcatg gagacccaga tcaccacact gatcatctcc
     3061 aagaaggagc gcatgctgca gaagggctct ggcttccacc tggatctgct gctgatcgtg
     3121 gccatgggcg gcatctgtgc cctgtttgga ctgccatggc tggcagcagc aaccgtgcgg
     3181 tccgtgacac acgcaaacgc actgacagtg atgtctaagg ccgtggcccc cggcgataag
     3241 cctaagatcc aggaggtgaa ggagcagcgc gtgaccggac tgctggtggc cctgctggtg
     3301 ggactgtcca tcgtgatcgg cgacctgctg aggcagatcc cactggccgt gctgttcggc
     3361 atctttctgt acatgggcgt gacctctctg aatggcatcc agttctatga gcggctgcac
     3421 ctgctgctga tgccccctaa gcaccacccc gacgtgacct acgtgaagaa ggtgcgcaca
     3481 ctgcggatgc acctgtttac cgccctgcag ctgctgtgcc tggccctgct gtgggccgtg
     3541 atgtccacag ccgcctctct ggccttccct tttatcctga tcctgaccgt gccactgcgc
     3601 atggtggtgc tgaccagaat cttcacagat agggagatga agtgtctgga cgccaacgag
     3661 gccgagcccg tgtttgatga gcgggagggc gtggacgagt ataatgagat gcccatgcct
     3721 gtg
//
