LOCUS       sequence-25396-e         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Nausch LW, Ledoux J, Bonev AD, Nelson MT, Dostmann WR
  TITLE     Differential patterning of cGMP in vascular smooth muscle cells 
            revealed by single GFP-linked biosensors.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):365-70.
  PUBMED    18165313
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Jan 8. 105(1):365-70."
COMMENT     Sequence Label: pRSETA-betaFlincG
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..98
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(102..110,111..125)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc
       61 atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg
      121 ataaggatcg atggggatcc gagctcgagg ccatgggcac cctgcgggat ttacagtacg
      181 cgctccagga gaagatcgag gagctgaggc agcgggatgc tctcatcgac gagctggagc
      241 tggagttgga tcagaaggac gaactgatcc agaagctgca gaacgagctg gacaagtacc
      301 gctcggtgat ccggccggcc acccagcagg cgcagaagca gagcgcgagc accttgcaag
      361 gcgagccgcg caccaagcgg caggcgatct ccgctgagcc caccgccttt gacatccagg
      421 atctcagcca tgtgaccctg cccttctacc ccaagagtcc acagtccaag gatctcataa
      481 aggaggccat ccttgacaat gactttatga agaacttgga gctgtcacag atccaagaga
      541 ttgtggattg tatgtaccca gtggagtacg gcaaagacag ctgcatcatc aaagaaggag
      601 atgtggggtc actggtgtat gtcat
//
