LOCUS       sequence-253968-        3411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC4A5
FEATURES             Location/Qualifiers
     source          1..3411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaaggtga aggaggagaa ggccggcgtg ggcaagctgg accacaccaa ccaccggaga
       61 aggttccccg atcagaagga gtgcccacct atccacatcg gactgccagt gcctacctac
      121 cctcagagaa agacagatca gaagggccac ctgtctggcc tgcagaaggt gcactgggga
      181 ctgaggccag accagccaca gcaggagctg accggacctg gaagcggagc aagctcccag
      241 gactctagca tggatctgat ctctagaaca aggagcccag cagcagagca gctgcaggac
      301 atcctgggag aggaggatga ggccccaaat cccaccctgt ttacagagat ggataccctg
      361 cagcacgacg gcgatcagat ggagtggaag gagagcgccc gctggatcaa gttcgaggag
      421 aaggtggagg agggaggaga gcggtggtcc aagccccacg tgagcaccct gtccctgcac
      481 tctctgttcg agctgagaac atgcctgcag accggcacag tgctgctgga cctggattct
      541 ggcagcctgc cacagatcat cgacgatgtg atcgagaagc agatcgagga cggactgctg
      601 aggccagagc tgagggagcg ggtgtcttac gtgctgctgc gccggcacag gcaccagacc
      661 aagaagccta tccaccggag cctggccgat atcggcaagt ccgtgtctac cacaaacaga
      721 tcccctgcaa ggtctccagg agcaggacca agcctgcacc actccacaga ggacctgaga
      781 atgaggcagt ccgccaacta tggccggctg tgccacgcac agagccggtc catgaatgac
      841 atctctctga cccctaacac agatcagcgg aagaataagt ttatgaagaa gatcccaaag
      901 gacagcgagg cctccaatgt gctggtgggc gaggtggact tcctggatca gcccttcatc
      961 gcctttgtga gactgatcca gtccgccatg ctgggaggag tgaccgaggt gcctgtgcca
     1021 acaaggttcc tgtttatcct gctgggccct agcggcagag ccaagtccta caacgagatc
     1081 ggcagggcca tcgccaccct gatggtggac gatctgttta gcgacgtggc ctataaggcc
     1141 cgcaatcggg aggatctgat cgccggcatc gacgagttcc tggatgaagt gatcgtgctg
     1201 ccaccaggag agtgggaccc aaacatcaga atcgagcctc caaagaaggt gcccagcgcc
     1261 gataagagga agagcgtgtt cagcctggcc gagctgggcc agatgaacgg ctccgtggga
     1321 ggaggaggag gagcaccagg aggaggcaat ggcggaggag gcggcggcgg ctctggcggc
     1381 ggcgccggca gcggcggcgc cggcggcacc tcctctggcg acgatggcga gatgcccgcc
     1441 atgcacgaga tcggcgagga gctgatctgg acaggccgct tctttggcgg cctgtgcctg
     1501 gatatcaagc ggaagctgcc ttggttccca tccgactttt acgatggctt ccacatccag
     1561 tctatcagcg ccatcctgtt tatctatctg ggctgtatca ccaacgccat cacattcggc
     1621 ggactgctgg gcgacgcaac cgataattac cagggcgtga tggagtcctt tctgggcaca
     1681 gccatggccg gctccctgtt ctgcctgttt tctggccagc ccctgatcat cctgagctcc
     1741 accggcccta tcctgatctt tgagaagctg ctgttcgact ttagcaaggg caacggcctg
     1801 gattatatgg agttccgcct gtggatcgga ctgcactccg ccgtgcagtg tctgatcctg
     1861 gtggccaccg acgcctcttt catcatcaag tacatcaccc ggtttacaga ggagggcttc
     1921 tctacactga tcagcttcat ctttatctat gacgccatca agaagatgat cggcgccttt
     1981 aagtactatc caatcaacat ggacttcaag cccaatttca tcaccacata caagtgcgag
     2041 tgcgtggccc ccgacaccgt gaacaccaca gtgttcaatg caagcgcccc actggcacct
     2101 gacaccaacg cctccctgta caacctgctg aatctgacag ccctggattg gtccctgctg
     2161 tctaagaagg agtgcctgtc ttatggcggc cgcctgctgg gcaatagctg taagtttatc
     2221 cctgatctgg ccctgatgtc cttcatcctg ttctttggca cctactctat gaccctgaca
     2281 ctgaagaagt tcaagtttag cagatatttc ccaacaaagg tgagggccct ggtggccgac
     2341 ttttccatcg tgttctctat cctgatgttt tgcggcatcg atgcctgttt cggactggag
     2401 accccaaagc tgcacgtgcc tagcgtgatc aagccaacaa gacccgacag gggctggttt
     2461 gtggccccat tcggcaagaa cccctggtgg gtgtatcctg cctccatcct gccagccctg
     2521 ctggtgacca tcctgatctt tatggatcag cagatcacag ccgtgatcgt gaacagaaag
     2581 gagaataagc tgaagaaggc cgccggctac cacctggacc tgttttgggt gggcatcctg
     2641 atggccctgt gcagcttcat gggcctgcct tggtacgtgg ccgccaccgt gatcagcatc
     2701 gcccacatcg attccctgaa gatggagacc gagacatccg cccctggaga gcagccacag
     2761 ttcctgggcg tgcgcgagca gagggtgacc ggcatcatcg tgtttatcct gacaggcatc
     2821 agcgtgttcc tggccccaat cctgaagtgt atccccctgc ctgtgctgta cggcgtgttt
     2881 ctgtatatgg gcgtggcctc tctgaatgga atccagatgg gaaccggagg aagcgagttc
     2941 aagatccaga agaagctgac acccttttgg gagcggtgca agctgttcct gatgcccgcc
     3001 aagcaccagc ctgaccacgc ctttctgcgg cacgtgcctc tgagaaggat ccacctgttc
     3061 accctggtgc agatcctgtg cctggccgtg ctgtggatcc tgaagtccac agtggccgcc
     3121 atcatctttc ccgtgatgat cctgggcctg atcatcgtgc gccggctgct ggatttcatc
     3181 ttttctcagc acgacctggc ctggatcgat aacatcctgc cagagaagga gaagaaggag
     3241 accgacaaga agagaaagag gaagaaggga gcacacgagg actgcgatga ggagccccag
     3301 ttcccccctc caagcgtgat caagatccct atggagagcg tgcagtccga cccacagaat
     3361 ggcatccact gtatcgcccg caagcggtct agctcctggt cctactctct g
//
