LOCUS       sequence-253990-        3834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-NPC1
FEATURES             Location/Qualifiers
     source          1..3834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaccgcaa ggggactggc cctgggactg ctgctgctgc tgctgtgccc agcccaggtg
       61 ttctctcaga gctgcgtgtg gtatggcgag tgtggcatcg cctacggcga caagcggtac
      121 aactgcgagt acagcggacc acctaagcca ctgccaaagg acggctacga tctggtgcag
      181 gagctgtgcc ctggcttctt tttcggcaac gtgtccctgt gctgtgacgt gcggcagctg
      241 cagaccctga aggataatct gcagctgcct ctgcagtttc tgtctagatg cccaagctgt
      301 ttttataacc tgctgaatct gttctgcgag ctgacctgtt ccccaaggca gtctcagttc
      361 ctgaacgtga ccgccacaga ggactacgtg gatcccgtga caaaccagac caagacaaat
      421 gtgaaggagc tgcagtacta tgtgggccag agcttcgcca acgccatgta taatgcatgc
      481 agggacgtgg aggcaccaag ctccaatgat aaggccctgg gactgctgtg cggcaaggac
      541 gcagatgcct gtaacgccac caattggatc gagtacatgt ttaacaagga taatggccag
      601 gcccctttca ccatcacacc agtgttttcc gacttccccg tgcacggcat ggagcctatg
      661 aacaatgcca ccaagggctg cgacgagtct gtggatgagg tgacagcccc ttgcagctgt
      721 caggattgct ccatcgtgtg cggacctaag ccacagccac cacctccacc agcaccatgg
      781 accatcctgg gactggacgc catgtatgtg atcatgtgga tcacatacat ggcctttctg
      841 ctggtgtttt tcggcgcctt tttcgccgtg tggtgctatc ggaagagata cttcgtgagc
      901 gagtacaccc ccatcgacag caacatcgcc ttcagcgtga atgcctccga caagggcgag
      961 gcatcctgct gtgatcccgt gagcgccgcc ttcgagggat gcctgcggag actgtttacc
     1021 cggtggggct ctttctgcgt gagaaaccca ggctgcgtga tctttttcag cctggtgttt
     1081 atcacagcct gttctagcgg cctggtgttc gtgagggtga ccacaaatcc agtggatctg
     1141 tggagcgccc catcctctca ggcaaggctg gagaaggagt actttgacca gcacttcggc
     1201 ccatttttca ggaccgagca gctgatcatc agggcaccac tgacagataa gcacatctac
     1261 cagccatatc ctagcggagc agacgtgccc ttcggccctc cactggatat ccagatcctg
     1321 caccaggtgc tggacctgca gatcgccatc gagaacatca ccgcctccta tgacaatgag
     1381 accgtgacac tgcaggatat ctgcctggcc cctctgagcc catataacac caattgtaca
     1441 atcctgtccg tgctgaacta ctttcagaat tcccactctg tgctggatca caagaagggc
     1501 gacgacttct tcgtgtacgc cgactatcac acccacttcc tgtactgcgt gcgggcccct
     1561 gcctctctga acgacaccag cctgctgcac gatccatgtc tgggcacatt tggcggaccc
     1621 gtgttccctt ggctggtgct gggcggctat gacgatcaga actacaacaa tgccaccgcc
     1681 ctggtcatca ccttccctgt gaacaattac tataatgata ccgagaagct gcagagagcc
     1741 caggcctggg agaaggagtt tatcaacttc gtgaagaatt acaagaaccc aaatctgacc
     1801 atcagcttca cagccgagcg gtccatcgag gacgagctga acagagagag cgactccgac
     1861 gtgttcaccg tggtcatcag ctatgccatc atgttcctgt acatcagcct ggccctgggc
     1921 cacatgaagt cctgcaggcg cctgctggtg gatagcaagg tgtccctggg catcgccggc
     1981 atcctgatcg tgctgagctc cgtggcctgt tccctgggcg tgttctctta tatcggcctg
     2041 cctctgaccc tgatcgtgat cgaagtgatc ccatttctgg tgctggccgt gggcgtggac
     2101 aatatcttca tcctggtgca ggcataccag agggacgagc gcctgcaggg agagacactg
     2161 gatcagcagc tgggaagggt gctgggagag gtggcaccat ccatgtttct gtctagcttc
     2221 tctgagaccg tggccttttt cctgggcgcc ctgtccgtga tgccagcagt gcacacattt
     2281 tctctgttcg ccggcctggc cgtgtttatc gacttcctgc tgcagatcac ctgcttcgtg
     2341 agcctgctgg gcctggacat caagaggcag gagaagaacc gcctggatat cttctgctgc
     2401 gtgcggggag cagaggacgg cacatccgtg caggcctctg agagctgtct gttccggttt
     2461 ttcaagaatt cctattctcc actgctgctg aaggactgga tgagacccat cgtgatcgcc
     2521 atctttgtgg gcgtgctgag cttctccatc gccgtgctga acaaggtgga catcggcctg
     2581 gatcagtctc tgagcatgcc cgacgatagc tatatggtgg attactttaa gtccatctct
     2641 cagtatctgc acgcaggacc acccgtgtac ttcgtgctgg aggagggcca cgactacacc
     2701 tcctctaagg gccagaatat ggtgtgcggc ggcatgggct gtaacaatga ttccctggtg
     2761 cagcagatct tcaacgccgc ccagctggac aattatacac ggatcggctt tgcccccagc
     2821 tcctggatcg acgattactt cgattgggtg aagcctcagt ctagctgctg tagagtggac
     2881 aacatcaccg atcagttttg caatgcaagc gtggtggacc ctgcatgcgt gaggtgtagg
     2941 ccactgacac cagagggcaa gcagaggcca cagggaggcg acttcatgcg cttcctgcct
     3001 atgttcctgt ccgacaaccc aaatccaaag tgtggcaagg gaggacacgc agcatactcc
     3061 tctgccgtga acatcctgct gggacacgga accagggtgg gagccacata cttcatgacc
     3121 taccacacag tgctgcagac ctccgccgac ttcatcgatg ccctgaagaa ggccaggctg
     3181 atcgcctcta acgtgaccga gacaatgggc atcaatggct ctgcctatcg cgtgtttccc
     3241 tacagcgtgt tttacgtgtt ctatgagcag tacctgacca tcatcgacga tacaatcttt
     3301 aacctgggcg tgagcctggg cgccatcttc ctggtgacca tggtgctgct gggatgcgag
     3361 ctgtggtccg ccgtgatcat gtgcgccaca atcgccatgg tgctggtgaa tatgttcggc
     3421 gtgatgtggc tgtggggcat cagcctgaac gccgtgtccc tggtgaacct ggtcatgtct
     3481 tgcggcatca gcgtggagtt ttgtagccac atcaccagag ccttcacagt gtccatgaag
     3541 ggatctaggg tggagagagc agaggaggcc ctggcacaca tgggaagctc cgtgttttct
     3601 ggcatcaccc tgacaaagtt cggcggcatc gtggtgctgg cctttgccaa gagccagatc
     3661 ttccagatct tttatttccg gatgtacctg gcaatggtgc tgctgggagc aacccacggc
     3721 ctgatctttc tgcccgtgct gctgtcttat atcggcccta gcgtgaacaa ggccaagtcc
     3781 tgcgccaccg aggagagata caagggcaca gagcgggaga gactgctgaa tttc
//
