LOCUS       sequence-254000-        3279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC4A8
FEATURES             Location/Qualifiers
     source          1..3279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcctgcag caggatctaa cgagccagac ggcgtgctga gctaccagag gccagacgag
       61 gaggcagtgg tggatcaggg aggcacctct acaatcctga atatccacta cgagaaggag
      121 gagctggagg gccaccggac actgtatgtg ggcgtgcgca tgccactggg ccggcagagc
      181 cacagacacc acaggaccca cggacagaag caccggagaa ggggaagggg caagggagca
      241 agccagggag aggagggcct ggaggccctg gcacacgaca caccttccca gcgggtgcag
      301 tttatcctgg gcaccgagga ggatgaggag cacgtgccac acgagctgtt cacagagctg
      361 gacgagatct gcatgaagga gggcgaggat gcagagtgga aggagaccgc aaggtggctg
      421 aagtttgagg aggacgtgga ggatggagga gagcggtggt ctaagcccta cgtggccaca
      481 ctgtccctgc actctctgtt cgagctgcgg agctgtctga tcaacggcac cgtgctgctg
      541 gacatgcacg ccaatagcat cgaggagatc tccgacctga tcctggatca gcaggagctg
      601 agctccgacc tgaacgatag catgagagtg aaggtgaggg aggccctgct gaagaagcac
      661 caccaccaga atgagaagaa gcggaacaat ctgatcccta tcgtgaggtc cttcgccgaa
      721 gtgggcaaga agcagtctga cccacacctg atggataagc acggacagac cgtgagccca
      781 cagtccgtgc ctaccacaaa cctggaggtg aagaacggcg tgaattgcga gcactctccc
      841 gtggacctga gcaaggtgga tctgcacttt atgaagaaga tccctaccgg agcagaggca
      901 agcaatgtgc tggtgggcga ggtggacatc ctggatcgcc caatcgtggc cttcgtgcgg
      961 ctgtctccag ccgtgctgct gagcggactg acagaggtgc ctatcccaac cagattcctg
     1021 tttatcctgc tgggaccagt gggcaaggga cagcagtacc acgagatcgg caggtccatg
     1081 gccaccatca tgacagacga gatctttcac gatgtggcct ataaggccaa ggagagagac
     1141 gatctgctgg ccggcatcga cgagttcctg gatcaggtga ccgtgctgcc acctggagag
     1201 tgggacccaa gcatccgcat cgagccaccc aagaacgtgc cctcccagga gaagcggaag
     1261 atgcccggcg tgcctaacgg caacgtgtgc cacatcgagc aggagccaca cggaggacac
     1321 tccggacctg agctgcagag gaccggccgg ctgtttggag gcctggtgct ggatatcaag
     1381 cgcaaggccc cctggtactg gagcgactat cgggatgccc tgtccctgca gtgtctggcc
     1441 tctttcctgt ttctgtactg cgcctgtatg tcccctgtga tcacattcgg cggactgctg
     1501 ggagaggcaa ccgagggaag gatctccgcc atcgagtctc tgtttggcgc ctccatgacc
     1561 ggcatcgcct attctctgtt cgcaggacag gccctgacaa tcctgggaag caccggaccc
     1621 gtgctggtgt ttgagaagat cctgttcaag ttttgcaagg actacgccct gtcttatctg
     1681 agcctgagag cctgcatcgg cctgtggaca gccttcctgt gcatcgtgct ggtggccacc
     1741 gatgcctcta gcctggtgtg ctacatcacc aggttcacag aggaggcctt tgcctccctg
     1801 atctgtatca tctttatcta cgaggccatc gagaagctga tccacctggc cgagacctat
     1861 cctatccaca tgcacagcca gctggaccac ctgtccctgt actattgcag atgtacactg
     1921 ccagagaatc ccaacaatca caccctgcag tattggaagg atcacaacat cgtgacagcc
     1981 gaggtgcact gggccaatct gaccgtgtct gagtgccagg agatgcacgg cgagttcatg
     2041 ggaagcgcct gtggacacca cggaccttac acaccagacg tgctgttttg gtcctgcatc
     2101 ctgttcttta ccacattcat cctgtcctct accctgaaga cattcaagac ctctaggtat
     2161 tttccaaccc gcgtgcggtc catggtgtct gacttcgccg tgtttctgac aatcttcacc
     2221 atggtcatca tcgattttct gatcggagtg ccaagcccta agctgcaggt gccttccgtg
     2281 tttaagccaa caagagacga taggggctgg atcatcaacc caatcggccc aaatccctgg
     2341 tggaccgtga tcgcagcaat catccccgcc ctgctgtgca caatcctgat cttcatggac
     2401 cagcagatca ccgccgtgat catcaaccgc aaggagcaca agctgaagaa gggctgcggc
     2461 taccacctgg atctgctgat ggtggccatc atgctgggcg tgtgcagcat catgggcctg
     2521 ccttggttcg tggccgccac agtgctgtct atcacccacg tgaatagcct gaagctggag
     2581 agcgagtgct ccgccccagg agagcagccc aagtttctgg gaatcaggga gcagagggtg
     2641 acaggactga tgatcttcgt gctgatgggc tgttccgtgt tcatgaccgc catcctgaag
     2701 tttatcccta tgccagtgct gtacggcgtg tttctgtata tgggcgtgag ctccctgcag
     2761 ggcatccagt tctttgaccg gctgaagctg tttggcatgc ccgccaagca ccagcctgat
     2821 ttcatctatc tgagacacgt gcctctgagg aaggtgcacc tgttcacact gatccagctg
     2881 acctgcctgg tgctgctgtg ggtcatcaag gcctccccag ccgccatcgt gttccccatg
     2941 atggtgctgg ccctggtgtt tgtgagaaaa gtgatggacc tgtgcttctc caagagggag
     3001 ctgtcttggc tggacgatct gatgccagag agcaagaaga agaagctgga cgatgccaag
     3061 aagaaggcca aggaggagga ggaggccgag aagatgctgg agatcggcgg cgataagttt
     3121 cctctggagt ccagaaagct gctgtctagc ccaggcaaga acatctcttg caggtgtgac
     3181 cccagcgaga tcaatatctc cgatgagatg cctaagacca cagtgtggaa ggccctgtct
     3241 atgaacagcg gcaatgccaa ggagaagagc ctgttcaac
//
