LOCUS       sequence-254006-        4077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-NPC1L1
FEATURES             Location/Qualifiers
     source          1..4077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2044..2053
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcagagg caggactgag gggatggctg ctgtgggccc tgctgctgcg cctggcccag
       61 agcgagccat acaccacaat ccaccagccc ggctactgtg ccttctatga cgagtgcggc
      121 aagaaccctg agctgtctgg cagcctgatg accctgagca acgtgtcctg tctgtctaat
      181 accccagccc ggaagatcac aggcgatcac ctgatcctgc tgcagaagat ctgccccaga
      241 ctgtataccg gccctaatac acaggcctgc tgtagcgcca agcagctggt gtccctggag
      301 gcctccctgt ctatcaccaa ggccctgctg acacggtgtc cagcctgcag cgacaacttc
      361 gtgaatctgc actgtcacaa cacctgctct cccaatcaga gcctgtttat caacgtgaca
      421 agagtggcac agctgggagc aggacagctg cctgccgtgg tggcctacga ggccttttat
      481 cagcactctt tcgccgagca gagctacgat agctgctccc gggtgagagt gccagcagca
      541 gccaccctgg cagtgggaac aatgtgcggc gtgtatggat ccgccctgtg caacgcacag
      601 cggtggctga atttccaggg cgacaccgga aatggactgg cacctctgga tatcacattt
      661 cacctgctgg agccaggaca ggcagtggga tctggaatcc agcccctgaa cgagggagtg
      721 gcaaggtgta atgagagcca gggcgacgat gtggccacat gttcttgcca ggactgtgca
      781 gcaagctgcc cagcaatcgc aaggccacag gccctggata gcaccttcta cctgggacag
      841 atgccaggat ccctggtgct gatcatcatc ctgtgcagcg tgttcgccgt ggtgacaatc
      901 ctgctggtgg gctttagggt ggcacctgca cgcgacaagt ccaagatggt ggatccaaag
      961 aagggcacct ctctgagcga caagctgtcc ttctctaccc acacactgct gggccagttc
     1021 tttcagggat ggggaacctg ggtggcaagc tggcccctga caatcctggt gctgtccgtg
     1081 atccctgtgg tggccctggc agcaggactg gtgtttaccg agctgaccac agaccctgtg
     1141 gagctgtggt ccgccccaaa ctctcaggcc aggagcgaga aggcctttca cgatcagcac
     1201 ttcggccctt tctttcgcac caaccaggtc atcctgacag ccccaaatag gagctcctac
     1261 cgctatgact ctctgctgct gggccctaag aacttcagcg gcatcctgga cctggatctg
     1321 ctgctggagc tgctggagct gcaggagagg ctgaggcacc tgcaagtgtg gagcccagag
     1381 gcacagcgga atatctccct gcaggatatc tgctacgccc cactgaaccc cgacaatacc
     1441 tctctgtacg attgctgtat caacagcctg ctgcagtatt ttcagaacaa tagaacactg
     1501 ctgctgctga ccgccaatca gacactgatg ggccagacca gccaggtgga ctggaaggat
     1561 cacttcctgt actgtgccaa cgcccctctg acctttaagg acggaacagc cctggccctg
     1621 tcctgcatgg ccgattatgg cgcccccgtg ttcccattcc tggccatcgg cggctacaag
     1681 ggcaaggact attccgaggc cgaggccctg atcatgacct tctctctgaa caattaccca
     1741 gcaggcgatc caaggctggc acaggcaaag ctgtgggagg aggcctttct ggaggagatg
     1801 cgcgccttcc agcggagaat ggccggcatg tttcaggtga cattcatggc cgagaggagc
     1861 ctggaggacg agatcaaccg caccacagcc gaggatctgc caatctttgc cacctcctac
     1921 atcgtgatct tcctgtatat ctccctggcc ctgggctctt actctagctg gagcagagtg
     1981 atggtggaca gcaaggccac cctgggactg ggaggagtgg cagtggtgct gggcgccgtg
     2041 atggccgcca tgggcttctt ttcttacctg ggcatccgct cctctctggt catcctgcag
     2101 gtggtgccct tcctggtgct gtccgtggga gccgataata tctttatctt cgtgctggag
     2161 tatcagcggc tgccaaggcg ccctggagag ccaagagagg tgcacatcgg acgggccctg
     2221 ggaagagtgg cacctagcat gctgctgtgc agcctgtccg aggccatctg cttctttctg
     2281 ggcgccctga caccaatgcc tgccgtgcgg accttcgccc tgacatccgg actggccgtg
     2341 atcctggact ttctgctgca gatgtctgcc ttcgtggccc tgctgagcct ggactccaag
     2401 cggcaggagg cctccagact ggacgtgtgc tgttgcgtga agccacagga gctgccacct
     2461 ccaggacagg gagagggcct gctgctgggc ttctttcaga aggcctacgc cccctttctg
     2521 ctgcactgga tcaccagggg cgtggtgctg ctgctgtttc tggccctgtt cggcgtgtct
     2581 ctgtatagca tgtgccacat cagcgtggga ctggatcagg agctggccct gcccaaggac
     2641 tcctacctgc tggattattt tctgttcctg aaccgctact ttgaagtggg cgcccccgtg
     2701 tacttcgtga ccacactggg ctataacttt agctccgagg ccggcatgaa tgccatctgt
     2761 tctagcgccg gctgcaacaa tttttccttc acccagaaga tccagtacgc cacagagttc
     2821 ccagagcagt cttatctggc catccccgcc tcctcttggg tggacgactt catcgactgg
     2881 ctgaccccta gctcctgttg cagactgtac atcagcggcc ccaacaagga taagttctgc
     2941 ccttccacag tgaactctct gaattgtctg aagaattgca tgtccatcac catgggaagc
     3001 gtgcggccat ccgtggagca gtttcacaag tacctgccct ggttcctgaa cgacagacct
     3061 aatatcaagt gtccaaaggg cggcctggcc gcctattcta ccagcgtgaa cctgacatct
     3121 gacggccagg tgctggatac cgtggccatc ctgagcccac ggctggagta ctctggcaca
     3181 atcagcgccc actgcaatct gtatctgctg gactccacct ctagattcat ggcctaccac
     3241 aagcccctga agaacagcca ggattatacc gaggccctga gggcagcaag agagctggca
     3301 gccaatatca ccgcagacct gaggaaggtg cctggaacag atccagcctt tgaggtgttc
     3361 ccctacacca tcacaaacgt gttttacgag cagtatctga caatcctgcc cgagggcctg
     3421 ttcatgctga gcctgtgcct ggtgcctacc tttgccgtgt cctgcctgct gctgggactg
     3481 gacctgagat ccggcctgct gaatctgctg tctatcgtga tgatcctggt ggatacagtg
     3541 ggcttcatgg ccctgtgggg catctcctat aacgccgtgt ctctgatcaa tctggtgagc
     3601 gccgtgggca tgtccgtgga gttcgtgtct cacatcaccc ggtcctttgc catctctacc
     3661 aagcccacat ggctggagag agccaaggag gccacaatct ccatgggatc tgccgtgttc
     3721 gcaggagtgg caatgaccaa cctgcctgga atcctggtgc tgggactggc aaaggcacag
     3781 ctgatccaga tcttcttttt caggctgaat ctgctgatca cactgctggg cctgctgcac
     3841 ggcctggtgt ttctgccagt gatcctgagc tacgtgggac cagacgtgaa ccctgccctg
     3901 gccctggagc agaagagggc agaggaggca gtggcagccg tgatggtggc ctcctgcccc
     3961 aatcacccta gccgcgtgtc caccgccgat aacatctatg tgaatcacag cttcgaggga
     4021 tccatcaagg gagcaggagc aatcagcaac ttcctgccca acaatggccg ccagttt
//
