LOCUS       sequence-254038-        3236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 1 module- CEN (Centromeric).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G
  TITLE     COSPLAY: An expandable toolbox for combinatorial and swift 
            generation of expression plasmids in yeast.
  JOURNAL   PLoS One. 2019 Aug 28;14(8):e0220694. doi: 
            10.1371/journal.pone.0220694. eCollection 2019.
  PUBMED    31461461
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-28"; volume: "14(8):e0220694. doi"; pages: " 
            10.1371/journal.pone.0220694. eCollection 2019"
COMMENT     Sequence Label: pMV13
FEATURES             Location/Qualifiers
     source          1..3236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..44
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     238..260
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     252..269
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     253..269
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    327..830
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(889..905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(889..905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(902..924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    913..929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(937..943,944..961,962..967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    982..1003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1120..1137)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1291..1879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1371..1390)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2050..2841,2842..2910))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2673..2692
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2911..3015)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3083..3101
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3139..3161)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
       61 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
      121 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      181 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      241 agtcacgacg ttgtaaaacg acggccagtg aattcgagct cggtacctcg cgaatgcatc
      301 tagatatcgg atcccggtct cgatccatca cgtgctataa aaataattat aatttaaatt
      361 ttttaatata aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata
      421 gtttttgttt tccgaagatg taaaagactc tagggggatc gccaacaaat actacctttt
      481 atcttgctct tcctgctctc aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa
      541 gaccacacac gaaaatcctg tgattttaca ttttacttat cgttaatcga atgtatatct
      601 atttaatctg cttttcttgt ctaataaata tatatgtaaa gtacgctttt tgttgaaatt
      661 ttttaaacct ttgtttattt ttttttcttc attccgtaac tcttctacct tctttattta
      721 ctttctaaaa tccaaataca aaacataaaa ataaataaac acagagtaaa ttcccaaatt
      781 attccatcat taaaagatac gaggcgcgtg taagttacag gcaagcgatc tatgagagac
      841 cgggcccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt catagctgtt
      901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      961 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1021 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1081 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1141 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1201 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1261 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1321 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1381 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1441 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1501 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1561 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1621 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1681 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1741 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1801 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1861 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1921 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1981 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2041 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2101 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2161 atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc cagatttatc
     2221 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2281 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2341 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2401 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2461 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2521 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2581 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2641 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2701 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2761 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2821 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2881 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2941 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3001 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     3061 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     3121 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     3181 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcg
//
