LOCUS       sequence-254039-        2828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 1 module- VI260998 (yeast integrative region VI260998).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G
  TITLE     COSPLAY: An expandable toolbox for combinatorial and swift 
            generation of expression plasmids in yeast.
  JOURNAL   PLoS One. 2019 Aug 28;14(8):e0220694. doi: 
            10.1371/journal.pone.0220694. eCollection 2019.
  PUBMED    31461461
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-28"; volume: "14(8):e0220694. doi"; pages: " 
            10.1371/journal.pone.0220694. eCollection 2019"
COMMENT     Sequence Label: pMV46
FEATURES             Location/Qualifiers
     source          1..2828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    97..118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(133..138,139..156,157..163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    171..187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     176..198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     195..211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     195..211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(423..440)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(423..439)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(432..454)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(648..667)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     767..789
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(827..845)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        913..1017
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1018..1086,1087..1878)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1236..1255)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2049..2637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2538..2557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2791..2808
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg catgcaggcc
      241 tctgcagtcg acgggcccgg tctctcatac ttctgtagct tggcatatac tgcaacaagt
      301 ccgtaatttg gccggccggc gcgccccttt cccttgtttt ttgcaactcc atgtattaat
      361 aaaagggatc gagaccggga tccgatatct agatgcattc gcgaggtacc gagctcgaat
      421 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      481 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      541 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
      601 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
      661 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      721 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      781 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
      841 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      901 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      961 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1021 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1081 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1141 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1201 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1261 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1321 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1381 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1441 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1501 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1561 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1621 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1681 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggatctcgc
     1741 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1801 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1861 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1921 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1981 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2041 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2101 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2161 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2221 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2281 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2341 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2401 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2461 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2521 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2581 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2641 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2701 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2761 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2821 cgcccaat
//
