LOCUS       sequence-254041-        6460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Flag-tagged human MCPIP1 full lengh protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Liang J, Saad Y, Lei T, Wang J, Qi D, Yang Q, Kolattukudy PE, Fu M
  TITLE     MCP-induced protein 1 deubiquitinates TRAF proteins and negatively 
            regulates JNK and NF-kappaB signaling.
  JOURNAL   J Exp Med. 2010 Dec 20;207(13):2959-73. doi: 10.1084/jem.20092641. 
            Epub 2010 Nov 29.
  PUBMED    21115689
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20092641"; journalName: "J Exp Med"; date: "2010-12-20-
            20"; volume: "207"; issue: "13"; pages: "2959-73"
COMMENT     Sequence Label: Flag-MCPIP1 (1-599)
FEATURES             Location/Qualifiers
     source          1..6460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             962..985
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2801..3423
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3000..3019)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3447..3467)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3452..3781
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3632..3767
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3819..3838)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3820..3838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3852..3868)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3852..3868)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3865..3887)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3876..3892
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3900..3906,3907..3924,3925..3930))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3945..3966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4083..4100)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4254..4842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4334..4353)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5013..5804,5805..5873))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5636..5655
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5874..5978)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6005..6433
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6092..6111)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6302..6323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggcccac aaccaccgcc acaagcacgc
      961 cgactacaaa gacgatgacg acaagcttat gagtggcccc tgtggagaga agcctgtcct
     1021 ggaagccagc cccaccatga gtctgtggga atttgaggac agccacagcc gtcagggcac
     1081 cccaaggccg ggtcaagagc tggccgctga ggaggcctcg gccctggaac tgcagatgaa
     1141 ggtggacttc ttccggaagc tgggctattc atccacggag atccacagcg tcctgcagaa
     1201 gctgggcgtc caggcagaca ccaacacggt gctgggtgag ctggtgaaac acgggacagc
     1261 caccgagcgg gagcgccaga cctcaccgga cccctgccct cagctccctc tagtcccgcg
     1321 gggtggtggc acccctaagg ctcccaacct ggagcctcca ctcccagaag aggaaaagga
     1381 gggcagcgac ctgagaccag tggtcatcga tgggagcaac gtggccatga gccatgggaa
     1441 caaggaggtc ttctcctgcc ggggcatcct gctggcagtg aactggtttc tggagcgggg
     1501 ccacacagac atcacagtgt ttgtgccatc ctggaggaag gagcagcctc ggcccgacgt
     1561 gcccatcaca gaccagcaca tcctgcggga actggagaag aagaagatcc tggtgttcac
     1621 accatcacga cgcgtgggtg gcaagcgggt ggtgtgctat gacgacagat tcattgtgaa
     1681 gctggcctac gagtctgacg ggatcgtggt ttccaacgac acataccgtg acctccaagg
     1741 cgagcggcag gagtggaagc gcttcatcga ggagcggctg ctcatgtact ccttcgtcaa
     1801 tgacaagttt atgccccctg atgacccact gggccggcac gggcccagcc tggacaactt
     1861 cctgcgtaag aagccactca ctttggagca caggaagcag ccgtgtccct atggaaggaa
     1921 atgcacctat gggatcaagt gccgattctt ccacccagag cggccaagct gcccccagcg
     1981 ctctgtggca gatgagctcc gtgccaatgc tctcctctca ccccccagag ccccaagcaa
     2041 ggacaaaaat ggccggcggc cttcaccttc atcccagtcc agctctctgc taacagagag
     2101 tgagcagtgc agcctggatg ggaagaagct gggggcccag gcatccccag ggtcccgcca
     2161 agagggtcta acacagacct atgccccatc aggcaggagc ctcgcaccta gcgggggcag
     2221 tggcagcagc tttgggccca cagactggct cccacagacg ctggactcac tcccgtacgt
     2281 ctcccaggat tgcctggact cgggcattgg ctccctggag agccagatgt cggaactttg
     2341 gggggttcga ggaggaggcc ctggtgagcc gggcccaccc cgagcccctt acacgggcta
     2401 cagtccctat ggatctgagc tcccagccac cgcagccttc tctgcctttg gccgggccat
     2461 gggtgctggc cacttcagtg tccctgccga ctacccaccc gcgccccctg cctttccacc
     2521 tcgagagtac tggtctgaac catacccact gcccccaccc acatcagtcc ttcaggagcc
     2581 cccagtgcag agcccagggg ctggcaggag cccgtggggc agggcaggca gcctggccaa
     2641 ggagcaggcc agcgtgtata ctaagctgtg tggtgtgttt cccccgcacc tggtggaggc
     2701 tgtgatgggg cgcttcccac agctcctgga cccccagcag ctggctgccg agatcctctc
     2761 ctacaagtcc cagcacccca gtgagtaaag atctgatccc gggtggcatc cctgtgaccc
     2821 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
     2881 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
     2941 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
     3001 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
     3061 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
     3121 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
     3181 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
     3241 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca
     3301 gcaggaggac gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt
     3361 gagttgcttg cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga
     3421 attgggtacg cggccagctt ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3481 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3541 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3601 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3661 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3721 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3781 aaagctcctc gaggaactga aaaaccagaa agttaattcc ctatagtgag tcgtattaaa
     3841 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3901 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3961 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4021 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4081 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4141 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4201 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4261 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4441 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4681 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4741 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5581 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5641 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5701 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5761 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5821 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6001 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6061 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6121 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6181 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6241 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6421 tttaacgcga attttaacaa aatattaacg cttacaattt
//
