LOCUS       sequence-254043-        5080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5' Entry 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5080)
  TITLE     –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5' Entry 
            vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p5E-2.3k-fabp2
FEATURES             Location/Qualifiers
     source          1..5080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(919..946)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1038..1124)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1173..1195
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1187..1204
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1188..1204
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1244..1339
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1341..1359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1384..1400
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1385..1401
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3705..3721)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3705..3721)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3758..3776)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3777..3900
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3973..3991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3996..4012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3996..4012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4114..4133)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4125..4934
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4192..4211)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      901 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      961 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     1021 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     1081 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     1141 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
     1201 cagtcttaag ctcgggcccg agttaacgct accatggagc tccaaataat gattttattt
     1261 tgactgatag tgacctgttc gttgcaacaa attgataagc aatgcttttt tataatgcca
     1321 actttgtata gaaaagttgg taatacgact cactataggg cgaattgggt accgggcccc
     1381 ccctcgaggt cgacggtatc gataagcttc ccttccagct gcaacccatc actgggaaac
     1441 actcatacac tcacagtcac acagtttagc ctacgcaatt cacctatata ttcatcacat
     1501 atctttggac ttgtggggga aaccggaaac tccacacaga aatgccaact gacccagctg
     1561 aggttcaaac cagcccttct tactgtgagg tgacagcgct agccattgcg ccaccgctcc
     1621 accctttatt ttaatttatt taattgaatt tattttattg tatttgtcct aaattgccag
     1681 tacaatcagt acaatcatta gaatggtttc tgaaggttca tgcctgtaat aactgcttta
     1741 tatttaaatt tgtctatagc aataagattt agtaataata ttaataattt tatattctat
     1801 tctattatta tattatatat acattttcac actaaaccga aaagagactt cttattaacg
     1861 acctcaaaaa aataactaaa tctgaaaaag atcactgtta cgcagattct gcaaattcta
     1921 caaaatgttt gtagtaaata tagtataaaa gatgttttta gcttactttt tacattattt
     1981 tatacattta aacatgcatg aaatcaatta ttatcagctt ggaccttttc ttgtccctac
     2041 tcttagtggg acgatgccaa aggtcttaca atgggacgat accaaaggtc ttcagaggtc
     2101 ttatttaatt gcactgttaa gagattaagg ataaggtaat gatgagataa accaccatca
     2161 gagattatca tgtctcagtt tgatatctcg tctctttatc ttgtcatgga gcctccaggt
     2221 aaaacttcca cactgcatgt tgcttaattt gaatcatttg caggctttgt acactattca
     2281 tcattacaga taatgctgaa gctgttcttt cacagccttg catcaatatt tacaacattt
     2341 tcaaacaata caaggggata attcagtaat acaatttaaa ataaggttcc aataactata
     2401 ttactaataa ctaacaatga gcaaaataca taatgctgtt attttagtat aactggatga
     2461 gtactgcaga ttttaaataa aatattcatg gaattactgt cgttactgta taatgttggt
     2521 gaatgtatgg tctgtttttg acatttgtgt gcatttattt ttatatctaa agtgttttat
     2581 agatttgcat tttaatatta gtagtaaatg caaatgataa atactttatc tttaaattaa
     2641 aaatattatt atgcattact tatctgtgat aaatatatcg tatgtgttat gttactgata
     2701 aaatgttaca ataataatgg ttcggttagg gttaaggttt taggacaaaa cacaactgtt
     2761 cattagtatt cagatcagat ctatgaaata atattggcag gtgcaaattt tcattttaat
     2821 aatgtactag tactgtaaat atttgagtta gctttagaac tggctttgaa gtatattcac
     2881 tggtagctgt ctgttaagta gaactgcaca aaaaaaaggt acgaggaaga acgaagcatt
     2941 gccatatact gtattctcaa ttttagctgc aatagctgca cttttgtagt aaaaagttct
     3001 tcaaagaccc tttttatata gtgtgaagaa cttttcaata atcttaaaac atttttctac
     3061 ataaataaaa tctttgtgca atgaaatatt tcaaaaagta ctttataaaa ccaaccattc
     3121 atgtcaacac agagccttaa aataattgtt accagtattt ctcaatagcc aatgaggcat
     3181 gcagcaagat tgtaaactaa tttaactgta accattaaat ctaaagtttt gacctttgac
     3241 ctttcaccac gattgtttaa aaatcaatac atgtggctag aattcatagc tttcaaacac
     3301 tcttcatagt ccctcagtag ttactgcaac acaaggcact tactagtata acagacacac
     3361 cctacacagt ttaaacagcg aacaattcaa acaaaagcag gactttgtta tgagaacaac
     3421 agagcgtgta tgtttgacca tcgcagtaca agtgagtgct tgttctcagg acttgaaatc
     3481 tccgctcttt gcacaatcaa tgaataagca aggcatgctg ggatgtgtgt aacatatagc
     3541 ctgttgggcg ggtgagattt atacttggtg agctttactc ggccacatca gcatgaagat
     3601 aatactcaga taaggcaacg cttcgccact cgcacaggta taaaagagtg gctcggggta
     3661 aagttaggcc actgtcagga tcacacaaca gtctgtcatc atcggatcca ctagttctag
     3721 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattcaag
     3781 tttgtacaaa aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     3841 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg
     3901 aaccaactac ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcg
     3961 gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     4021 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     4081 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     4141 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     4201 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     4261 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     4321 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     4381 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     4441 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     4501 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     4561 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     4621 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     4681 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     4741 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     4801 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     4861 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     4921 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     4981 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     5041 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
