LOCUS       sequence-254045-       12332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian cell expression of Q61L mutant murine RAC1 carrying a 
            modified hormone-binding domain of murine ERalpha at the N-terminus.
            Activation of expressed latent fusion protein induced by 4-OHT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12332)
  AUTHORS   Lionarons DA, Hancock DC, Rana S, East P, Moore C, Murillo MM, 
            Carvalho J, Spencer-Dene B, Herbert E, Stamp G, Damry D, Calado DP, 
            Rosewell I, Fritsch R, Neubig RR, Molina-Arcas M, Downward J
  TITLE     RAC1(P29S) Induces a Mesenchymal Phenotypic Switch via Serum 
            Response Factor to Promote Melanoma Development and Therapy 
            Resistance.
  JOURNAL   Cancer Cell. 2019 Jul 8;36(1):68-83.e9. doi: 
            10.1016/j.ccell.2019.05.015. Epub 2019 Jun 27.
  PUBMED    31257073
REFERENCE   2  (bases 1 to 12332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2019.05.015"; journalName: "Cancer Cell"; date: 
            "2019-07-8- 8"; volume: "36"; issue: "1"; pages: "68-83.e9"
COMMENT     Sequence Label: pLZRS-ER-Rac1-Q61L-shortlinker
FEATURES             Location/Qualifiers
     source          1..12332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             3490..3959
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4089..4106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4260..4848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4340..4359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4971..5105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4995..5014)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5049..5068
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5335..5934)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5418..5438)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5915..5934
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6017..6516)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6065..6084)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6458..6481
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      8548..10337
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(10831..11622,11623..11691))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11454..11473
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11692..11796)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg gatccaccat gcgaaatgaa atgggtgctt caggagacat
     1801 gagggctgcc aacctttggc caagccctct tgtgattaag cacactaaga agaatagccc
     1861 tgccttgtcc ttgacagctg accagatggt cagtgccttg ttggatgctg aaccgcccat
     1921 gatctattct gaatatgatc cttctagacc cttcagtgaa gcctcaatga tgggcttatt
     1981 gaccaaccta gcagataggg agctggttca tatgatcaac tgggcaaaga gagtgccagg
     2041 ctttggggac ttgaatctcc atgatcaggt ccaccttctc gagtgtgcct ggctggagat
     2101 tctgatgatt ggtctcgtct ggcgctccat ggaacacccg gggaagctcc tgtttgctcc
     2161 taacttgctc ctggacagga atcaaggtaa atgtgtggaa ggcatggtgg agatctttga
     2221 catgttgctt gctacgtcaa gtcggttccg catgatgaac ctgcagggtg aagagtttgt
     2281 gtgcctcaaa tccatcattt tgcttaattc cggagtgtac acgtttctgt ccagcacctt
     2341 gaagtctctg gaagagaagg accacatcca ccgtgtcctg gacaagatca cagacacttt
     2401 gatccacctg atggccaaag ctggcctgac tctgcagcag cagcatcgcc gcctagctca
     2461 gctccttctc attctttccc atatccggca tatgagtaac aaacgcatgg agcatctcta
     2521 caacatgaaa tgcaagaacg tggtacccct ctatgacctg ctcctggaga tgttggatgc
     2581 ccaccgcctt catgccccag ccagtcgcat gggagtgccc ccagaggagc ccagccagac
     2641 ccagctggcc accaccagct ccacttcagc acattcctta caaacctact acataccccc
     2701 ggaagcagag ggcttcccca acgatcaggc catcaagtgt gtggtggtgg gagacggagc
     2761 tgttggtaaa acctgcctgc tcatcagtta cacgaccaat gcatttcctg gagagtacat
     2821 ccccaccgtc tttgacaact attctgccaa tgttatggta gatggaaaac cagtgaatct
     2881 gggcctatgg gacacagctg gactagaaga ttatgacaga ttgcgtcccc tctcctaccc
     2941 gcagacagac gtgttcttaa tttgcttttc ccttgtgagt cctgcatcat ttgaaaatgt
     3001 ccgtgcaaag tggtatcctg aagtgcgaca ccactgtccc aatactccta tcatcctcgt
     3061 ggggacgaag cttgatctta gggatgataa ggacaccatt gagaagctga aggagaagaa
     3121 gctgactccc atcacctacc cgcaggggct ggccatggcg aaagagatcg gtgctgtcaa
     3181 atacctggag tgctcagctc tcacacagcg aggactcaag acagtgtttg acgaagctat
     3241 ccgagcggtt ctctgtcccc ctcctgtcaa gaagaggaag agaaaatgcc tgctgttgta
     3301 agcggccgcc agcacagtgg tcgacgataa aataaaagat tttatttagt ctccagaaaa
     3361 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     3421 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     3481 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     3541 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     3601 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     3661 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     3721 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     3781 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     3841 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     3901 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     3961 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     4021 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     4081 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4141 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4201 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4261 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4321 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4381 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4441 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4501 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4561 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4621 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4681 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4741 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4801 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4861 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4921 atgagattat caaaaaggat cttcacctag atccttttgc ggccgaatta gatccagaca
     4981 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5041 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5101 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     5161 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagagt
     5221 cggtgggcct cgggggcggg tgcggggtcg gcggggccgc cccgggtggc ttcggtcgga
     5281 gccatggggt cgtgcgctcc tttcggtcgg gcgctgcggg tcgtggggcg ggcgtcaggc
     5341 accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt
     5401 gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa
     5461 ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag
     5521 acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg cgggctcctt
     5581 gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct
     5641 caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg
     5701 cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag
     5761 cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg
     5821 gtcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc
     5881 ccgggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggaagg
     5941 tcgtctcctt gtgaggggtc aggggcgtgg gtcaggggat ggtggcggca ccggtcgtgg
     6001 cggccgacct gcaggtcgaa aggcccggag atgaggaaga ggagaacagc gcggcagacg
     6061 tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg cccgccccgc
     6121 ccctgagccc gcccctgagc ccgcccccgg acccacccct tcccagcctc tgagcccaga
     6181 aagcgaagga gcaaagctgc tattggccgc tgccccaaag gcctacccgc ttccattgct
     6241 cagcggtgct gtccatctgc acgagactag tgagacgtgc tacttccatt tgtcacgtcc
     6301 tgcacgacgc gagctgcggg gcggggggga acttcctgac taggggagga gtagaaggtg
     6361 gcgcgaaggg gccaccaaag aacggagccg gttggcgcct accggtggat gtggaatgtg
     6421 tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag cggggctgct aaagcgcatg
     6481 ctccagactg ccttgggaaa agcgcctccc ctacccggta gaattaattc tcatgtttga
     6541 cagcttatca tcgataagct gatcctcaca ggccgcaccc agcttttctt ccgttgcccc
     6601 agtagcatct ctgtctggtg accttgaaga ggaagaggag gggtcccgag aatccccatc
     6661 cctaccgtcc agcaaaaagg gggacgagga atttgaggcc tggcttgagg ctcaggacgc
     6721 aaatcttgag gatgttcagc gggagttttc cgggctgcga gtaattggtg atgaggacga
     6781 ggatggttcg gaggatgggg aattttcaga cctggatctg tctgacagcg accatgaagg
     6841 ggatgagggt gggggggctg ttggaggggg caggagtctg cactccctgt attcactgag
     6901 cgtcgtctaa taaagatgtc tattgatctc ttttagtgtg aatcatgtct gacgaggggc
     6961 caggtacagg acctggaaat ggcctaggag agaagggaga cacatctgga ccagaaggct
     7021 ccggcggcag tggacctcaa agaagagggg gtgataacca tggacgagga cggggaagag
     7081 gacgaggacg aggaggcgga agaccaggag ccccgggcgg ctcaggatca gggccaagac
     7141 atagagatgg tgtccggaga ccccaaaaac gtccaagttg cattggctgc aaagggaccc
     7201 acggtggaac aggagcagga gcaggagcgg gaggggcagg agcaggaggg gcaggagcag
     7261 gaggaggggc aggagcagga ggggcaggag caggaggtgg aggccggggt cgaggaggca
     7321 gtggaggccg gggtcgagga ggtagtggag gccggggtcg aggaggtagt ggaggccgcc
     7381 ggggtagagg acgtgaaaga gccagggggg gaagtcgtga aagagccagg gggagaggtc
     7441 gtggacgtgg agaaaagagg cccaggagtc ccagtagtca gtcatcatca tccgggtctc
     7501 caccgcgcag gccccctcca ggtagaaggc catttttcca ccctgtaggg gaagccgatt
     7561 attttgaata ccaccaagaa ggtggcccag atggtgagcc tgacgtgccc ccgggagcga
     7621 tagagcaggg ccccgcagat gacccaggag aaggcccaag cactggaccc cggggtcagg
     7681 gtgatggagg caggcgcaaa aaaggagggt ggtttggaaa gcatcgtggt caaggaggtt
     7741 ccaacccgaa atttgagaac attgcagaag gtttaagagc tctcctggct aggagtcacg
     7801 tagaaaggac taccgacgaa ggaacttggg tcgccggtgt gttcgtatat ggaggtagta
     7861 agacctccct ttacaaccta aggcgaggaa ctgcccttgc tattccacaa tgtcgtctta
     7921 caccattgag tcgtctcccc tttggaatgg cccctggacc cggcccacaa cctggcccgc
     7981 taagggagtc cattgtctgt tatttcatgg tctttttaca aactcatata tttgctgagg
     8041 ttttgaagga tgcgattaag gaccttgtta tgacaaagcc cgctcctacc tgcaatatca
     8101 gggtgactgt gtgcagcttt gacgatggag tagatttgcc tccctggttt ccacctatgg
     8161 tggaaggggc tgccgcggag ggtgatgacg gagatgacgg agatgaagga ggtgatggag
     8221 atgagggtga ggaagggcag gagtgatgta acttgttagg agacgccctc aatcgtatta
     8281 aaagccgtgt attcccccgc actaaagaat aaatccccag tagacatcat gcgtgctgtt
     8341 ggtgtatttc tggccatctg tcttgtcacc attttcgtcc tcccaacatg gggcaattgg
     8401 gcatacccat gttgtcacgt cactcagctc cgcgctcaac accttctcgc gttggaaaac
     8461 attagcgaca tttacctggt gagcaatcag acatgcgacg gctttagcct ggcctcctta
     8521 aattcaccta agaatgggag caaccagcag gaaaaggaca agcagcgaaa attcacgccc
     8581 ccttgggagg tggcggcata tgcaaaggat agcactccca ctctactact gggtatcata
     8641 tgctgactgt atatgcatga ggatagcata tgctacccgg atacagatta ggatagcata
     8701 tactacccag atatagatta ggatagcata tgctacccag atatagatta ggatagccta
     8761 tgctacccag atataaatta ggatagcata tactacccag atatagatta ggatagcata
     8821 tgctacccag atatagatta ggatagccta tgctacccag atatagatta ggatagcata
     8881 tgctacccag atatagatta ggatagcata tgctatccag atatttgggt agtatatgct
     8941 acccagatat aaattaggat agcatatact accctaatct ctattaggat agcatatgct
     9001 acccggatac agattaggat agcatatact acccagatat agattaggat agcatatgct
     9061 acccagatat agattaggat agcctatgct acccagatat aaattaggat agcatatact
     9121 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct
     9181 acccagatat agattaggat agcatatgct atccagatat ttgggtagta tatgctaccc
     9241 atggcaacat tagcccaccg tgctctcagc gacctcgtga atatgaggac caacaaccct
     9301 gtgcttggcg ctcaggcgca agtgtgtgta atttgtcctc cagatcgcag caatcgcgcc
     9361 cctatcttgg cccgcccacc tacttatgca ggtattcccc ggggtgccat tagtggtttt
     9421 gtgggcaagt ggtttgaccg cagtggttag cggggttaca atcagccaag ttattacacc
     9481 cttattttac agtccaaaac cgcagggcgg cgtgtggggg ctgacgcgtg cccccactcc
     9541 acaatttcaa aaaaaagagt ggccacttgt ctttgtttat gggccccatt ggcgtggagc
     9601 cccgtttaat tttcgggggt gttagagaca accagtggag tccgctgctg tcggcgtcca
     9661 ctctctttcc ccttgttaca aatagagtgt aacaacatgg ttcacctgtc ttggtccctg
     9721 cctgggacac atcttaataa ccccagtatc atattgcact aggattatgt gttgcccata
     9781 gccataaatt cgtgtgagat ggacatccag tctttacggc ttgtccccac cccatggatt
     9841 tctattgtta aagatattca gaatgtttca ttcctacact agtatttatt gcccaagggg
     9901 tttgtgaggg ttatattggt gtcatagcac aatgccacca ctgaaccccc cgtccaaatt
     9961 ttattctggg ggcgtcacct gaaaccttgt tttcgagcac ctcacataca ccttactgtt
    10021 cacaactcag cagttattct attagctaaa cgaaggagaa tgaagaagca ggcgaagatt
    10081 caggagagtt cactgcccgc tccttgatct tcagccactg cccttgtgac taaaatggtt
    10141 cactaccctc gtggaatcct gaccccatgt aaataaaacc gtgacagctc atggggtggg
    10201 agatatcgct gttccttagg acccttttac taaccctaat tcgatagcat atgcttcccg
    10261 ttgggtaaca tatgctattg aattagggtt agtctggata gtatatacta ctacccggga
    10321 agcatatgct acccgtttag ggttaacaag ggggccttat aaacactatt gctaatgccc
    10381 tcttgagggt ccgcttatcg gtagctacac aggcccctct gattgacgtt ggtgtagcct
    10441 cccgtagtct tcctgggccc ctgggaggta catgtccccc agcattggtg taagagcttc
    10501 agccaagagt tacacataaa ggcaatgttg tgttgcagtc cacagactgc aaagtctgct
    10561 ccaggatgaa agccactcag tgttggcaaa tgtgcacatc catttataag gatgtcaact
    10621 acagtcagag aacccctttg tgtttggtcc ccccccgtgt cacatgtgga acagggccca
    10681 gttggcaagt tgtaccaacc aactgaaggg attacatgca ctgccccgcg aagaaggggc
    10741 agagatgtcg tagtcaggtt tagttcgtcc ggggcgggga tctggccgca aatcaatcta
    10801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    10921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    11041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    11101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    11161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    11221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    11281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    11341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    11401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    11461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    11521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    11581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    11701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    11761 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    11821 agctttgctc ttaggagttt cctaatacat cccaaactca aatatataaa gcatttgact
    11881 tgttctatgc cctagggggc ggggggaagc taagccagct ttttttaaca tttaaaatgt
    11941 taattccatt ttaaatgcac agatgttttt atttcataag ggtttcaatg tgcatgaatg
    12001 ctgcaatatt cctgttacca aagctagtat aaataaaaat agataaacgt ggaaattact
    12061 tagagtttct gtcattaacg tttccttcct cagttgacaa cataaatgcg ctgctgagaa
    12121 gccagtttgc atctgtcagg atcaatttcc cattatgcca gtcatattaa ttactagtca
    12181 attagttgat ttttattttt gacatataca tgtgaaagac cccacctgta ggtttggcaa
    12241 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagaaaa
    12301 gttcagatca aggtcaggaa cagatggaac ag
//
