LOCUS       sequence-254055-       10871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Woodsmith J, Apelt L, Casado-Medrano V, Ozkan Z, Timmermann B, 
            Stelzl U
  TITLE     Protein interaction perturbation profiling at amino-acid resolution.
  JOURNAL   Nat Methods. 2017 Dec;14(12):1213-1221. doi: 10.1038/nmeth.4464. 
            Epub 2017 Oct 16.
  PUBMED    29039417
REFERENCE   2  (bases 1 to 10871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4464"; journalName: "Nat Methods"; date: "2017-12"; 
            volume: "14"; issue: "12"; pages: "1213-1221"
COMMENT     Sequence Label: pBTMcC24-DM
FEATURES             Location/Qualifiers
     source          1..10871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(369..1160,1161..1229))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     992..1011
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1230..1334)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1402..1420
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1458..1480)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1580..1599
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      1616..2780
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        2785..3187
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     protein_bind    3223..3347
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3521..3623
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3624..4283
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3690..3709)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4625..4930
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTADMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4971..5095)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(5103..5351,5352..5708)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="KALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEH
                     LKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSLF
                     KPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGNK
                     VELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     5643..5664
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     terminator      5736..5923
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5791..5815)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5976..6650)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(6651..6752)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(6766..6782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6766..6782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6779..6801)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6790..6806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6814..6820,6821..6838,6839..6844))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6859..6880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10310..10327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10481..10871,1..198))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10561..10580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
       61 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
      121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
      181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
      241 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
      301 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
      361 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
      421 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
      481 tctggcccca gtgctgcaat gataccgcga gayccacgct caccggctcc agatttatca
      541 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
      601 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
      661 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      721 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
      781 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
      841 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
      901 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
      961 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     1021 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     1081 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     1141 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     1201 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     1261 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     1321 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     1381 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     1441 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1501 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1561 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccataacgca
     1621 tttaagcata aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg
     1681 cagatatagg tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg
     1741 aagcgctcgt tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagcta
     1801 gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag acgcactttc
     1861 aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa taccgcttcc
     1921 acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc cctatataac
     1981 ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta cattttttat
     2041 gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat tcatagagtg
     2101 aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga gacaaaatag
     2161 aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca ctttctgttc
     2221 acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt tatcttgaaa
     2281 aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc aggctttttt
     2341 tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga cctacagtgc
     2401 aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag taatctaaga
     2461 tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa aagaagtata
     2521 gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct
     2581 cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta caaaaatgaa
     2641 gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc tgtaaaaatg
     2701 cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttctacaaaa
     2761 tgaagcacag atgcttcgtt gcttgcatgc aacttctttt cttttttttt tcttttctct
     2821 ctcccccgtt gttgtctcac catatccgca atgacaaaaa aatgatggaa gacactaaag
     2881 gaaaaaatta acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg
     2941 tatctcgaag cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag
     3001 caacggtata cggccttcct tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc
     3061 ttgctatcaa gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct
     3121 cgttcccttt cttccttgtt tctttttctg cacaatattt caagctatac caagcataca
     3181 atcaactcca agcttgaatt aattccgggc ggagtcgaga tgacaagttt gtacaaaaaa
     3241 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     3301 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcgggtg
     3361 atgctgccaa cttagcggcc gctaagttgg cagcatcacc cgacgcactt tgcgccgaat
     3421 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
     3481 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
     3541 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     3601 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     3661 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     3721 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     3781 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     3841 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     3901 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     3961 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     4021 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     4081 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     4141 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     4201 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     4261 atgagtggca gggcggggcg taatctagag gatccggctt actaaaagcc agataacagt
     4321 atgcgtattt gcgcgctgat tttcgcggta taagaatata tactgatatg tatacccgaa
     4381 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     4441 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     4501 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     4561 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     4621 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     4681 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     4741 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     4801 aagctggcgc atgatgaccg ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     4861 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     4921 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     4981 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     5041 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgggcc
     5101 gcaaagcgtt aacggccagg caacaagagg tgtttgatct catccgtgat cacatcagcc
     5161 agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa
     5221 acgcggctga agaacatctg aaggcgctgg cacgcaaagg cgttattgaa attgtttccg
     5281 gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga agggttgccg ctggtaggtc
     5341 gtgtggctgc cggtgaacca cttctggcgc aacagcatat tgaaggtcat tatcaggtcg
     5401 atccttcctt attcaagccg aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga
     5461 aagatatcgg cattatggat ggtgacttgc tggcagtgca taaaactcag gatgtacgta
     5521 acggtcaggt cgttgtcgca cgtattgatg acgaggttac cgttaagcgc ctgaaaaaac
     5581 agggcaataa agtcgaactg ttgccagaaa atagcgagtt taaaccaatt gtcgtagatc
     5641 ttcgtcagca gagcttcacc attgaagggc tggcggttgg ggttattcgc aacggcgact
     5701 ggctgtaacg gccgctgcag ccaagctaat tccgggcgaa tttcttatga tttatgattt
     5761 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     5821 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     5881 aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgccg gcaagtgcac
     5941 aaacaatact taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa
     6001 tttgctattt tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca
     6061 ccaataacgc catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct
     6121 aacataaaat gtaagctttc ggggctctct tgccttccaa cccagtcaga aatcgagttc
     6181 caatccaaaa gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga
     6241 ggtttctgtg aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta
     6301 ataactggca aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg
     6361 acgatatcaa tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga
     6421 aacacgccaa ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt
     6481 gaaattttcc ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata
     6541 cccagcaagt cagcatcgga atctagagca cattctgcgg cctctgtgct ctgcaagccg
     6601 caaactttca ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct
     6661 gcctttgtgt gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct
     6721 tggccctctc cttttctttt ttcgaccgaa ttaattcgta atcatgtcat agctgtttcc
     6781 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6841 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6901 cgctttccag tcgggaaacc tgtcgtgcca ggggctgcag tggagggtgt tgttgtggag
     6961 ttttccaaca ccttcaaatc gaagaaaact ggatctcttt ccgatttgga tagcaattga
     7021 ttttgttctt caagaacttt gaacattttg cccagcctag taatagctgg ttcgtaggga
     7081 gacgtctcac aatcatatgg gaatacaaat acaaaattaa aactaaaaaa attcctagca
     7141 tatattggag aattgattgt cacaggataa catacaatcc tatatgtacc atacttaaaa
     7201 gtgatcaact tgtggcataa tataggcttc ggtatgatgt agtttttaaa tgtgttgaaa
     7261 ttaataccat gatttttcaa attatttggc ggaaactcgt atttaatctt agaaccttct
     7321 gtaggatgga acacggagta aaatattgtg tgtatgggca caaacccttg gaaatagctt
     7381 agcatttgcc gttggcctga tgatctgacc attcccttta gtagagaaag aaagtcgctt
     7441 caagctaacc cagttttctt tttttttatc acttatcagt cctattcgga gatacaggca
     7501 acaagtgata gagggcccat tatgaatacg cacctctatg tatttccgag atacgattac
     7561 tccagttcct cttacaagaa atgcataaaa atagttacaa ttaattagaa caagaactta
     7621 tttagaacac gttcacactg agtaagaact cttgtccctt attagccttg atagtgctga
     7681 aaaaaagaaa aaaaacaaaa aaaagaaata aaataacggc aaacagcaaa ggccacagaa
     7741 ccgtattcat gttacttctg caatatcaat cacttactgg caagtgcgta taaattaaac
     7801 ctatttcttt atcatcatat ttacttatat ctttaacaga ttccaaaccc taaagtgtcc
     7861 gaattttcaa tagggcgaac ttgaagaata accaaggtca ataatatatc ttttagtata
     7921 accctgaaat ttgccctata gaaatctagg gtttctgtgt ggtttccggg tgagtcatac
     7981 ggcttttttg aatttctttt tttgcagttg tctctatcaa tgaaaatttc gaggaagacg
     8041 ataaggttaa gataagtaga taagagaatg atacgagata aagcacaaat tagcagaaag
     8101 aagagtggtt gcgaacagag taaaccgaat cagggaatcc ctttttgcaa aaacatcaat
     8161 tatccttttc ttttttttac gtatatatct ggaacagaaa tatataagtt actattatac
     8221 ttatagttgg atccagtttt taatctgtcg tcaatcgaaa gtttatttca gagttcttca
     8281 gacttcttaa ctcctgtaaa aacaaaaaaa aaaaaaggca tagcaatgac aaattcaaaa
     8341 gaagacgccg acatagagga gaagcatatg tacaatgagc cggtcacaac cctctttcac
     8401 gacgttgaag cttcacaaac acaccacaga cgtgggtcaa taccattgaa agatgagaaa
     8461 agtaaagaat tgtatccatt gcgctctttc ccgacgagag taaatggcga ggatacgttc
     8521 tctatggagg atggcatagg tgatgaagat gaaggagaag tacagaacgc tgaagtgaag
     8581 agagagctta agcaaagaca tattggtatg attgcccttg gtggtactat tggtacaggt
     8641 cttttcattg gtttatccac acctctgacc aacgccggcc cagtgggcgc tcttatatca
     8701 tatttattta tgggttcttt ggcatattct gtcacgcagt ccttgggtga aatggctaca
     8761 ttcatccctg ttacatcctc tttcacagtt ttctcacaaa gattcctttc tccagcattt
     8821 ggtgcggcca atggttacat gtattggttt tcttgggcaa tcacttttgc cctggaactt
     8881 agtgtagttg gccaagtcat tcaattttgg acgtacaaag ttccactggc ggcatggatt
     8941 agtatttttt gggtaattat cacaataatg aacttgttcc ctgtcaaata ttacggtgaa
     9001 ttcgagttct gggtcgcttc catcaaagtt ttagccatta tcgggtttct aatatactgt
     9061 ttttgtatgg tttgtggtgc tggggttacc ggcccagttg gattccgtta ttggagaaac
     9121 ccaggtgcct ggggtccagg tataatatct aaggataaaa acgaagggag gttcttaggt
     9181 tgggtttcct ctttgattaa cgctgccttc acatttcaag gtactgaact agttggtatc
     9241 actgctggtg aagctgcaaa ccccagaaaa tccgttccaa gagccatcaa aaaagttgtt
     9301 ttccgtatct taaccttcta cattggctct ctattattca ttggactttt agttccatac
     9361 aatgacccta aactaacaca atctacttcc tacgtttcta cttctccctt tattattgct
     9421 attgagaact ctggtacaaa ggttttgcca catatcttca acgctgttat cttaacaacc
     9481 attatttctg ccgcaaattc aaatatttac gttggttccc gtattttatt tggtctatca
     9541 aagaacaagt tggctcctaa attcctgtca aggaccacca aaggtggtgt tccatacatt
     9601 gcagttttcg ttactgctgc atttggcgct ttggcttaca tggagacatc tactggtggt
     9661 gacaaagttt tcgaatggct attaaatatc actggtgttg caggcttttt tgcatggtta
     9721 tttatctcaa tctcgcacat cagatttatg caagctttga aataccgtgg catctctcgt
     9781 gacgagttac catttaaagc taaattaatg cccggcttgg cttattatgc ggccacattt
     9841 atgacgatca ttatcattat tcaaggtttc acggcttttg caccaaaatt caatggtgtt
     9901 agctttgctg ccgcctatat ctctattttc ctgttcttag ctgtttggat cttatttcaa
     9961 tgcatattca gatgcagatt tatttggaag attggagatg tcgatcgaca tcgattccga
    10021 tagaagagac attgaggcaa ttgtatggga agatcatgaa ccaaagactt tttgggacaa
    10081 attttggaat gttgtagcat agatatgacg ttttattacc tttgatcaca tttccacgcc
    10141 atttcgcatt ctcaccctca taagtcatac accgaaaaga aagtttaagg gatcaatgag
    10201 cttactataa tctcagtata tttattttta tcgataagct tgatctgcat taatgaatcg
    10261 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    10321 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10381 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10441 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10501 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10561 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10621 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10681 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10741 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10801 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10861 ggtatgtagg c
//
