LOCUS       sequence-254057-        8529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Grossmann A, Benlasfer N, Birth P, Hegele A, Wachsmuth F, Apelt L, 
            Stelzl U
  TITLE     Phospho-tyrosine dependent protein-protein interaction network.
  JOURNAL   Mol Syst Biol. 2015 Mar 26;11(3):794.
  PUBMED    25814554
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2015-03-26"; volume: "11(3)"; pages: "794"
COMMENT     Sequence Label: pASZ-CN-DM
FEATURES             Location/Qualifiers
     source          1..8529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(446..1034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(526..545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1205..1996,1997..2065))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1828..1847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2066..2170)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2238..2256
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2294..2316)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2416..2435
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2644..2660
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2950..2970
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    2998..3122
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3296..3398
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3399..4058
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3465..3484)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4400..4705
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTADMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4746..4870)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4887..5134
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4903..4921)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5152..5171)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5153..5171)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5181..5197)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5425..5444)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(5485..5501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5485..5501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5498..5520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5509..5525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5533..5539,5540..5557,5558..5563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5578..5599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5665..6040
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             6041..7756
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     primer_bind     complement(7976..7993)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcacagaagt gtgtatatat atatttatat atagtcgatg tgcaaaagcc tacattattt
       61 ttcttttgat ttttattttt ttgttcaagt aggttcatgc cgacagtaaa tccttatgcc
      121 ctacctaaat ataaacaccc aaatatatat atatatatat atatatataa ataataaaaa
      181 cggaaaggaa atagaataat aataataata ctacacgttt acgtatgtaa ctcattctat
      241 tattcagact ttgatattaa tagaattatt actactaggt ttcctcactt caaaatctat
      301 aaatataccg tcatgctcta atcgatgctc actcaaaggc ggtaatacgg ttatccacag
      361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      961 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1321 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1801 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2161 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2221 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     2281 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2341 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2401 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2461 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2521 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2581 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2641 gttgtaaaac gacggccagt gaattcgagc tccttttgct ggcatttctt ctagaagcaa
     2701 aaagagcgat gcgtcttttc cgctgaaccg ttccagcaaa aaagactacc aacgcaatat
     2761 ggattgtcag aatcatataa aagagaagca aataactcct tgtcttgtat caattgcatt
     2821 ataatatctt cttgttagtg caatatcata tagaagtcat cgaaatagat attaagaaaa
     2881 acaaactgta caatcaatca atcatcacat aaaatggggt cgaggaaagc ggaattaatt
     2941 cccgagcctc caaaaaagaa gagaaaggtc gaattgggta ccgcgtcgac ggatctcaca
     3001 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
     3061 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
     3121 tggcggccgc gggtgatgct gccaacttag cggccgctaa gttggcagca tcacccgacg
     3181 cactttgcgc cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg
     3241 gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc
     3301 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     3361 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     3421 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     3481 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     3541 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     3601 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     3661 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     3721 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     3781 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     3841 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     3901 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     3961 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     4021 tacaacagta ctgcgatgag tggcagggcg gggcgtaatc tagaggatcc ggcttactaa
     4081 aagccagata acagtatgcg tatttgcgcg ctgattttcg cggtataaga atatatactg
     4141 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
     4201 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     4261 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     4321 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     4381 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     4441 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     4501 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
     4561 gcatatcggg gatgaaagct ggcgcatgat gaccgccgat atggccagtg tgccggtctc
     4621 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     4681 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     4741 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     4801 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
     4861 acaaagtggt aagtaactaa ctcgagtcat gtaattagtt atgtcacgct tacattcacg
     4921 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     4981 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     5041 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     5101 gaaggttttg ggacgctcga aggctttaat ttgcggccgg tacccaattc gccctatagt
     5161 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5221 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     5281 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     5341 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5401 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5461 tcgcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5521 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5581 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5641 gtcgtgccag cagatctgaa ttaattcttg aataatacat aacttttctt aaaagaatca
     5701 aagacagata aaatttaaga gatattaaat attagtgaga agccgagaat tttgtaacac
     5761 caacataaca ctgacatctt taacaacttt taattatgat acatttctta cgtcatgatt
     5821 gattattaca gctatgctga caaatgactc ttgttgcatg gctacgaacc gggtaatact
     5881 aagtgattga ctcttgctga ccttttatta agaactaaat ggacaatatt atggagcatt
     5941 tcatgtataa attggtgcgt aaaatcgttg gatctctctt ctaagtacat cctactataa
     6001 caatcaagaa aaacaagaaa atcggacaaa acaatcaagt atggattcta gaacagttgg
     6061 tatattagga gggggacaat tgggacgtat gattgttgag gcagcaaaca ggctcaacat
     6121 taagacggta atactagatg ctgaaaattc tcctgccaaa caaataagca actccaatga
     6181 ccacgttaat ggctcctttt ccaatcctct tgatatcgaa aaactagctg aaaaatgtga
     6241 tgtgctaacg attgagattg agcatgttga tgttcctaca ctaaagaatc ttcaagtaaa
     6301 acatcccaaa ttaaaaattt acccttctcc agaaacaatc agattgatac aagacaaata
     6361 tattcaaaaa gagcatttaa tcaaaaatgg tatagcagtt acccaaagtg ttcctgtgga
     6421 acaagccagt gagacgtccc tattgaatgt tggaagagat ttgggttttc cattcgtctt
     6481 gaagtcgagg actttggcat acgatggaag aggtaacttc gttgtaaaga ataaggaaat
     6541 gattccggaa gctttggaag tactgaagga tcgtcctttg tacgccgaaa aatgggcacc
     6601 atttactaaa gaattagcag tcatgattgt gaggtctgtt aacggtttag tgttttctta
     6661 cccaattgta gagactatcc acaaggacaa tatttgtgac ttatgttatg cgcctgctag
     6721 agttccggac tccgttcaac ttaaggcgaa gttgttggca gaaaatgcaa tcaaatcttt
     6781 tcccggttgt ggtatatttg gtgtggaaat gttctattta gaaacagggg aattgcttat
     6841 taacgaaatt gccccaaggc ctcacaactc tggacattat accattgatg cttgcgtcac
     6901 ttctcaattt gaagctcatt tgagatcaat attggatttg ccaatgccaa agaatttcac
     6961 atctttctcc accattacaa cgaacgccat tatgctaaat gttcttggag acaaacatac
     7021 aaaagataaa gagctagaaa cttgcgaaag agcattggcg actccaggtt cctcagtgta
     7081 cttatatgga aaagagtcta gacctaacag aaaagtaggt cacataaata ttattgcctc
     7141 cagtatggcg gaatgtgaac aaaggctgaa ctacattaca ggtagaactg atattccaat
     7201 caaaatctct gtcgctcaaa agttggactt ggaagcaatg gtcaaaccat tggttggaat
     7261 catcatggga tcagactctg acttgccggt aatgtctgcc gcatgtgcgg ttttaaaaga
     7321 ttttggcgtt ccatttgaag tgacaatagt ctctgctcat agaactccac ataggatgtc
     7381 agcatatgct atttccgcaa gcaagcgtgg aattaaaaca attatcgctg gagctggtgg
     7441 ggctgctcac ttgccaggta tggtggctgc aatgacacca cttcctgtca tcggtgtgcc
     7501 cgtaaaaggt tcttgtctag atggagtaga ttctttacat tcaattgtgc aaatgcctag
     7561 aggtgttcca gtagctaccg tcgctattaa taatagtacg aacgctgcgc tgttggctgt
     7621 cagactgctt ggcgcttatg attcaagtta tacaacgaaa atggaacagt ttttattaaa
     7681 gcaagaagaa gaagttcttg tcaaagcaca aaagttagaa actgtcggtt acgaagctta
     7741 tctagaaaac aagtaatata taagtttatt gatatacttg tacagcaaat aattataaaa
     7801 tgatatacct attttttagg ctttgttatg attacatcaa atgtggactt catacataga
     7861 aatcaacgct tacaggtgtc cttttttaag aatttcatac ataagatctg ctgcattaat
     7921 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7981 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagca tcgatgctag
     8041 agtcgaatgg aaattacgta ctttcaacgc aagagcaaga caaaagaaag cctaaaactg
     8101 tcttttcgtg tcggtcgtcc aatatcatcg taaacgtgtg tagagcctta aattctaatt
     8161 tctaacttta aatttttttt gctattataa tactaatttc taactttcaa cctattttac
     8221 atcttcggaa aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat
     8281 ttatatattt atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaaga
     8341 aatatagtct tcaaggttat attatacagg tttatatatt attttactta tttttgttgt
     8401 tctttttctt ggagtattta gcattaacaa cttcgaccca tcgcgatgac gaaattgcac
     8461 gtttaaagga gaagtcggaa tcgttgcaag aagaaataaa ttagtttgac gttttattcc
     8521 ttttcttct
//
