LOCUS       sequence-254081-       11710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA S332_L334delSAL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-S332_L334delSAL
FEATURES             Location/Qualifiers
     source          1..11710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6647..6671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6724..6765
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6786..7353
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6803..7355
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6947..6964)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7174..7193
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7367..7369,7370..7372,7373..8086)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7367..7369,7370..7372,7373..8083)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7412..7433)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7673..7692)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8020..8041
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8100..8688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8153..8173)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8571..8582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8763..8996
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9115..9133)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9201..9305
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9306..9374,9375..10166)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9524..9543)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10337..10925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10826..10845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11079..11096
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11336..11357
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11372..11377,11378..11395,11396..11402)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11410..11426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11415..11437
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11434..11450
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11434..11450
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11500..11519
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg
     3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata
     3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa
     3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta
     3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac
     3721 cttcggcttt ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgaagaaaag
     3781 atcctcaatc gagaaattgg ttttgctatc ggcatgccag tgtgtgaatt tgatatggtt
     3841 aaagatccag aagtacagga cttccgaaga aatattctga acgtttgtaa agaagctgtg
     3901 gatcttaggg acctcaattc acctcatagt agagcaatgt atgtctatcc tccaaatgta
     3961 gaatcttcac cagaattgcc aaagcacata tataataaat tagataaagg gcaaataata
     4021 gtggtgatct gggtaatagt ttctccaaat aatgacaagc agaagtatac tctgaaaatc
     4081 aaccatgact gtgtaccaga acaagtaatt gctgaagcaa tcaggaaaaa aactcgaagt
     4141 atgttgctat cctctgaaca actaaaactc tgtgttttag aatatcaggg caagtatatt
     4201 ttaaaagtgt gtggatgtga tgaatacttc ctagaaaaat atcctctgag tcagtataag
     4261 tatataagaa gctgtataat gcttgggagg atgcccaatt tgatgttgat ggctaaagaa
     4321 agcctttatt ctcaactgcc aatggactgt tttacaatgc catcttattc cagacgcatt
     4381 tccacagcta caccatatat gaatggagaa acatctacaa aatccctttg ggttataaat
     4441 agaataaaaa ttctttgtgc aacctacgtg aatgtaaata ttcgagacat tgataagatc
     4501 tatgttcgaa caggtatcta ccatggagga gaacccttat gtgacaatgt gaacactcaa
     4561 agagtacctt gttccaatcc caggtggaat gaatggctga attatgatat atacattcct
     4621 gatcttcctc gtgctgctcg actttgcctt tccatttgct ctgttaaagg ccgaaagggt
     4681 gctaaagagg aacactgtcc attggcatgg ggaaatataa acttgtttga ttacacagac
     4741 actctagtat ctggaaaaat ggctttgaat ctttggccag tacctcatgg attagaagat
     4801 ttgctgaacc ctattggtgt tactggatca aatccaaata aagaaactcc atgcttagag
     4861 ttggagtttg actggttcag cagtgtggta aagttcccag atatgtcagt gattgaagag
     4921 catgccaatt ggtctgtatc ccgagaagca ggatttagct attcccacgc aggactgagt
     4981 aacagactag ctagagacaa tgaattaagg gaaaatgaca aagaacagct caaagcaatt
     5041 tctacacgag atcctctctc tgaaatcact gagcaggaga aagattttct atggagtcac
     5101 agacactatt gtgtaactat ccccgaaatt ctacccaaat tgcttctgtc tgttaaatgg
     5161 aattctagag atgaagtagc ccagatgtat tgcttggtaa aagattggcc tccaatcaaa
     5221 cctgaacagg ctatggaact tctggactgt aattacccag atcctatggt tcgaggtttt
     5281 gctgttcggt gcttggaaaa atatttaaca gatgacaaac tttctcagta tttaattcag
     5341 ctagtacagg tcctaaaata tgaacaatat ttggataact tgcttgtgag atttttactg
     5401 aagaaagcat tgactaatca aaggattggg cactttttct tttggcattt aaaatctgag
     5461 atgcacaata aaacagttag ccagaggttt ggcctgcttt tggagtccta ttgtcgtgca
     5521 tgtgggatgt atttgaagca cctgaatagg caagtcgagg caatggaaaa gctcattaac
     5581 ttaactgaca ttctcaaaca ggagaagaag gatgaaacac aaaaggtaca gatgaagttt
     5641 ttagttgagc aaatgaggcg accagatttc atggatgctc tacagggctt tctgtctcct
     5701 ctaaaccctg ctcatcaact aggaaacctc aggcttgaag agtgtcgaat tatgtcctct
     5761 gcaaaaaggc cactgtggtt gaattgggag aacccagaca tcatgtcaga gttactgttt
     5821 cagaacaatg agatcatctt taaaaatggg gatgatttac ggcaagatat gctaacactt
     5881 caaattattc gtattatgga aaatatctgg caaaatcaag gtcttgatct tcgaatgtta
     5941 ccttatggtt gtctgtcaat cggtgactgt gtgggactta ttgaggtggt gcgaaattct
     6001 cacactatta tgcaaattca gtgcaaaggc ggcttgaaag gtgcactgca gttcaacagc
     6061 cacacactac atcagtggct caaagacaag aacaaaggag aaatatatga tgcagccatt
     6121 gacctgttta cacgttcatg tgctggatac tgtgtagcta ccttcatttt gggaattgga
     6181 gatcgtcaca atagtaacat catggtgaaa gacgatggac aactgtttca tatagatttt
     6241 ggacactttt tggatcacaa gaagaaaaaa tttggttata aacgagaacg tgtgccattt
     6301 gttttgacac aggatttctt aatagtgatt agtaaaggag cccaagaatg cacaaagaca
     6361 agagaatttg agaggtttca ggagatgtgt tacaaggctt atctagctat tcgacagcat
     6421 gccaatctct tcataaatct tttctcaatg atgcttggct ctggaatgcc agaactacaa
     6481 tcttttgatg acattgcata cattcgaaag accctagcct tagataaaac tgagcaagag
     6541 gctttggagt atttcatgaa acaaatgaat gatgcacatc atggtggctg gacaacaaaa
     6601 atggattgga tcttccacac aattaaacag catgcattga actagaaccc agctttcttg
     6661 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc
     6721 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa
     6781 tgagatccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     6841 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     6901 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     6961 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     7021 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     7081 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     7141 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     7201 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     7261 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     7321 gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatgg tgagcaaggg
     7381 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     7441 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     7501 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     7561 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     7621 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     7681 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     7741 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     7801 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     7861 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     7921 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     7981 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     8041 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaatcg atagatccta
     8101 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     8161 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     8221 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     8281 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     8341 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     8401 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     8461 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     8521 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     8581 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     8641 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt
     8701 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     8761 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     8821 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8881 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8941 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     9001 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     9061 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
     9121 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     9181 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9241 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9301 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9361 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9421 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9481 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9541 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9601 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9661 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9721 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9781 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9841 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9901 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9961 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10021 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10081 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10141 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10201 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10261 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10321 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10381 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10441 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10501 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10561 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10621 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10681 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10741 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10801 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10861 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10921 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10981 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11041 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11101 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11161 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    11221 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    11281 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11341 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11401 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
    11461 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    11521 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    11581 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    11641 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    11701 acttagtagt
//
