LOCUS       sequence-254083-       11669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA P449_N457delPHGLEDLLN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-P449_N457delPHGLEDLLN
FEATURES             Location/Qualifiers
     source          1..11669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6629..6653)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6661..6679
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6704..6723)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6705..6723)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6729..6749)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6803..6844
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6865..7432
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6882..7434
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7026..7043)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7253..7272
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7446..8045
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7446..7465)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7942..7962
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8059..8647
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8112..8132)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8530..8541)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8722..8955
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9074..9092)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9160..9264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9265..9333,9334..10125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9483..9502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10296..10884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10785..10804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11038..11055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11295..11316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11331..11336,11337..11354,11355..11361)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11369..11385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11374..11396
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11393..11409
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11393..11409
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11459..11478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg
     3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata
     3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa
     3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta
     3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac
     3721 cttcggcttt ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgaagaaaag
     3781 atcctcaatc gagaaattgg ttttgctatc ggcatgccag tgtgtgaatt tgatatggtt
     3841 aaagatccag aagtacagga cttccgaaga aatattctga acgtttgtaa agaagctgtg
     3901 gatcttaggg acctcaattc acctcatagt agagcaatgt atgtctatcc tccaaatgta
     3961 gaatcttcac cagaattgcc aaagcacata tataataaat tagataaagg gcaaataata
     4021 gtggtgatct gggtaatagt ttctccaaat aatgacaagc agaagtatac tctgaaaatc
     4081 aaccatgact gtgtaccaga acaagtaatt gctgaagcaa tcaggaaaaa aactcgaagt
     4141 atgttgctat cctctgaaca actaaaactc tgtgttttag aatatcaggg caagtatatt
     4201 ttaaaagtgt gtggatgtga tgaatacttc ctagaaaaat atcctctgag tcagtataag
     4261 tatataagaa gctgtataat gcttgggagg atgcccaatt tgatgttgat ggctaaagaa
     4321 agcctttatt ctcaactgcc aatggactgt tttacaatgc catcttattc cagacgcatt
     4381 tccacagcta caccatatat gaatggagaa acatctacaa aatccctttg ggttataaat
     4441 agtgcactca gaataaaaat tctttgtgca acctacgtga atgtaaatat tcgagacatt
     4501 gataagatct atgttcgaac aggtatctac catggaggag aacccttatg tgacaatgtg
     4561 aacactcaaa gagtaccttg ttccaatccc aggtggaatg aatggctgaa ttatgatata
     4621 tacattcctg atcttcctcg tgctgctcga ctttgccttt ccatttgctc tgttaaaggc
     4681 cgaaagggtg ctaaagagga acactgtcca ttggcatggg gaaatataaa cttgtttgat
     4741 tacacagaca ctctagtatc tggaaaaatg gctttgaatc tttggccagt acctattggt
     4801 gttactggat caaatccaaa taaagaaact ccatgcttag agttggagtt tgactggttc
     4861 agcagtgtgg taaagttccc agatatgtca gtgattgaag agcatgccaa ttggtctgta
     4921 tcccgagaag caggatttag ctattcccac gcaggactga gtaacagact agctagagac
     4981 aatgaattaa gggaaaatga caaagaacag ctcaaagcaa tttctacacg agatcctctc
     5041 tctgaaatca ctgagcagga gaaagatttt ctatggagtc acagacacta ttgtgtaact
     5101 atccccgaaa ttctacccaa attgcttctg tctgttaaat ggaattctag agatgaagta
     5161 gcccagatgt attgcttggt aaaagattgg cctccaatca aacctgaaca ggctatggaa
     5221 cttctggact gtaattaccc agatcctatg gttcgaggtt ttgctgttcg gtgcttggaa
     5281 aaatatttaa cagatgacaa actttctcag tatttaattc agctagtaca ggtcctaaaa
     5341 tatgaacaat atttggataa cttgcttgtg agatttttac tgaagaaagc attgactaat
     5401 caaaggattg ggcacttttt cttttggcat ttaaaatctg agatgcacaa taaaacagtt
     5461 agccagaggt ttggcctgct tttggagtcc tattgtcgtg catgtgggat gtatttgaag
     5521 cacctgaata ggcaagtcga ggcaatggaa aagctcatta acttaactga cattctcaaa
     5581 caggagaaga aggatgaaac acaaaaggta cagatgaagt ttttagttga gcaaatgagg
     5641 cgaccagatt tcatggatgc tctacagggc tttctgtctc ctctaaaccc tgctcatcaa
     5701 ctaggaaacc tcaggcttga agagtgtcga attatgtcct ctgcaaaaag gccactgtgg
     5761 ttgaattggg agaacccaga catcatgtca gagttactgt ttcagaacaa tgagatcatc
     5821 tttaaaaatg gggatgattt acggcaagat atgctaacac ttcaaattat tcgtattatg
     5881 gaaaatatct ggcaaaatca aggtcttgat cttcgaatgt taccttatgg ttgtctgtca
     5941 atcggtgact gtgtgggact tattgaggtg gtgcgaaatt ctcacactat tatgcaaatt
     6001 cagtgcaaag gcggcttgaa aggtgcactg cagttcaaca gccacacact acatcagtgg
     6061 ctcaaagaca agaacaaagg agaaatatat gatgcagcca ttgacctgtt tacacgttca
     6121 tgtgctggat actgtgtagc taccttcatt ttgggaattg gagatcgtca caatagtaac
     6181 atcatggtga aagacgatgg acaactgttt catatagatt ttggacactt tttggatcac
     6241 aagaagaaaa aatttggtta taaacgagaa cgtgtgccat ttgttttgac acaggatttc
     6301 ttaatagtga ttagtaaagg agcccaagaa tgcacaaaga caagagaatt tgagaggttt
     6361 caggagatgt gttacaaggc ttatctagct attcgacagc atgccaatct cttcataaat
     6421 cttttctcaa tgatgcttgg ctctggaatg ccagaactac aatcttttga tgacattgca
     6481 tacattcgaa agaccctagc cttagataaa actgagcaag aggctttgga gtatttcatg
     6541 aaacaaatga atgatgcaca tcatggtggc tggacaacaa aaatggattg gatcttccac
     6601 acaattaaac agcatgcatt gaactagaac ccagctttct tgtacaaagt ggtgatatcc
     6661 aattaaccct cactaaaggg ttagttatga tgaatattgt gtaccctata gtgagtcgta
     6721 ttaccaccca acttttctat acaaagtggt tgatatccag cacagtggcg gccgctcgag
     6781 tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
     6841 tacgcgtacc ggttagtaat gagatccctc ccccccccct aacgttactg gccgaagccg
     6901 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     6961 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     7021 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     7081 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     7141 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     7201 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     7261 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     7321 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     7381 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     7441 cacatatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     7501 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     7561 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     7621 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     7681 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     7741 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     7801 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     7861 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     7921 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     7981 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     8041 cctaaatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8101 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8161 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8221 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8281 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8341 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8401 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8461 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8521 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8581 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8641 tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat cttagccact
     8701 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     8761 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8821 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8881 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8941 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     9001 agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct
     9061 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     9121 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     9181 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9241 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     9301 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     9361 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     9421 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9481 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     9541 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     9601 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     9661 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     9721 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9781 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     9841 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     9901 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9961 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    10021 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    10081 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    10141 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10201 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10261 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    10321 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    10381 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    10441 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10501 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10561 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    10621 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10681 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10741 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10801 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10861 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10921 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10981 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    11041 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    11101 tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg
    11161 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    11221 taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt
    11281 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    11341 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    11401 agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    11461 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
    11521 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
    11581 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
    11641 tgttaaatat gtactacaaa cttagtagt
//
