LOCUS       sequence-254085-       11684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA V105_R108delVGNR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-V105_R108delVGNR
FEATURES             Location/Qualifiers
     source          1..11684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6644..6668)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6676..6694
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6719..6738)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6720..6738)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6744..6764)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6818..6859
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6880..7447
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6897..7449
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7041..7058)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7268..7287
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7461..8060
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7461..7480)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7957..7977
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8074..8662
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8127..8147)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8545..8556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8737..8970
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9089..9107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9175..9279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9280..9348,9349..10140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9498..9517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10311..10899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10800..10819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11053..11070
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11310..11331
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11346..11351,11352..11369,11370..11376)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11384..11400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11389..11411
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11408..11424
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11408..11424
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11474..11493
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg
     3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata
     3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa
     3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta
     3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac
     3721 cttcggcttt ttcaaccctt tttaaaagta attgaaccag aagaaaagat cctcaatcga
     3781 gaaattggtt ttgctatcgg catgccagtg tgtgaatttg atatggttaa agatccagaa
     3841 gtacaggact tccgaagaaa tattctgaac gtttgtaaag aagctgtgga tcttagggac
     3901 ctcaattcac ctcatagtag agcaatgtat gtctatcctc caaatgtaga atcttcacca
     3961 gaattgccaa agcacatata taataaatta gataaagggc aaataatagt ggtgatctgg
     4021 gtaatagttt ctccaaataa tgacaagcag aagtatactc tgaaaatcaa ccatgactgt
     4081 gtaccagaac aagtaattgc tgaagcaatc aggaaaaaaa ctcgaagtat gttgctatcc
     4141 tctgaacaac taaaactctg tgttttagaa tatcagggca agtatatttt aaaagtgtgt
     4201 ggatgtgatg aatacttcct agaaaaatat cctctgagtc agtataagta tataagaagc
     4261 tgtataatgc ttgggaggat gcccaatttg atgttgatgg ctaaagaaag cctttattct
     4321 caactgccaa tggactgttt tacaatgcca tcttattcca gacgcatttc cacagctaca
     4381 ccatatatga atggagaaac atctacaaaa tccctttggg ttataaatag tgcactcaga
     4441 ataaaaattc tttgtgcaac ctacgtgaat gtaaatattc gagacattga taagatctat
     4501 gttcgaacag gtatctacca tggaggagaa cccttatgtg acaatgtgaa cactcaaaga
     4561 gtaccttgtt ccaatcccag gtggaatgaa tggctgaatt atgatatata cattcctgat
     4621 cttcctcgtg ctgctcgact ttgcctttcc atttgctctg ttaaaggccg aaagggtgct
     4681 aaagaggaac actgtccatt ggcatgggga aatataaact tgtttgatta cacagacact
     4741 ctagtatctg gaaaaatggc tttgaatctt tggccagtac ctcatggatt agaagatttg
     4801 ctgaacccta ttggtgttac tggatcaaat ccaaataaag aaactccatg cttagagttg
     4861 gagtttgact ggttcagcag tgtggtaaag ttcccagata tgtcagtgat tgaagagcat
     4921 gccaattggt ctgtatcccg agaagcagga tttagctatt cccacgcagg actgagtaac
     4981 agactagcta gagacaatga attaagggaa aatgacaaag aacagctcaa agcaatttct
     5041 acacgagatc ctctctctga aatcactgag caggagaaag attttctatg gagtcacaga
     5101 cactattgtg taactatccc cgaaattcta cccaaattgc ttctgtctgt taaatggaat
     5161 tctagagatg aagtagccca gatgtattgc ttggtaaaag attggcctcc aatcaaacct
     5221 gaacaggcta tggaacttct ggactgtaat tacccagatc ctatggttcg aggttttgct
     5281 gttcggtgct tggaaaaata tttaacagat gacaaacttt ctcagtattt aattcagcta
     5341 gtacaggtcc taaaatatga acaatatttg gataacttgc ttgtgagatt tttactgaag
     5401 aaagcattga ctaatcaaag gattgggcac tttttctttt ggcatttaaa atctgagatg
     5461 cacaataaaa cagttagcca gaggtttggc ctgcttttgg agtcctattg tcgtgcatgt
     5521 gggatgtatt tgaagcacct gaataggcaa gtcgaggcaa tggaaaagct cattaactta
     5581 actgacattc tcaaacagga gaagaaggat gaaacacaaa aggtacagat gaagttttta
     5641 gttgagcaaa tgaggcgacc agatttcatg gatgctctac agggctttct gtctcctcta
     5701 aaccctgctc atcaactagg aaacctcagg cttgaagagt gtcgaattat gtcctctgca
     5761 aaaaggccac tgtggttgaa ttgggagaac ccagacatca tgtcagagtt actgtttcag
     5821 aacaatgaga tcatctttaa aaatggggat gatttacggc aagatatgct aacacttcaa
     5881 attattcgta ttatggaaaa tatctggcaa aatcaaggtc ttgatcttcg aatgttacct
     5941 tatggttgtc tgtcaatcgg tgactgtgtg ggacttattg aggtggtgcg aaattctcac
     6001 actattatgc aaattcagtg caaaggcggc ttgaaaggtg cactgcagtt caacagccac
     6061 acactacatc agtggctcaa agacaagaac aaaggagaaa tatatgatgc agccattgac
     6121 ctgtttacac gttcatgtgc tggatactgt gtagctacct tcattttggg aattggagat
     6181 cgtcacaata gtaacatcat ggtgaaagac gatggacaac tgtttcatat agattttgga
     6241 cactttttgg atcacaagaa gaaaaaattt ggttataaac gagaacgtgt gccatttgtt
     6301 ttgacacagg atttcttaat agtgattagt aaaggagccc aagaatgcac aaagacaaga
     6361 gaatttgaga ggtttcagga gatgtgttac aaggcttatc tagctattcg acagcatgcc
     6421 aatctcttca taaatctttt ctcaatgatg cttggctctg gaatgccaga actacaatct
     6481 tttgatgaca ttgcatacat tcgaaagacc ctagccttag ataaaactga gcaagaggct
     6541 ttggagtatt tcatgaaaca aatgaatgat gcacatcatg gtggctggac aacaaaaatg
     6601 gattggatct tccacacaat taaacagcat gcattgaact agaacccagc tttcttgtac
     6661 aaagtggtga tatccaatta accctcacta aaggtgatat agtagtgaag aaataagtcc
     6721 ctatagtgag tcgtattacc acccaacttt tctatacaaa gtggttgata tccagcacag
     6781 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
     6841 cctcggtctc gattctacgc gtaccggtta gtaatgagat ccctcccccc cccntaacgt
     6901 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     6961 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     7021 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     7081 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     7141 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     7201 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     7261 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     7321 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     7381 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     7441 atgataatat ggccacacat atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     7501 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     7561 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     7621 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     7681 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     7741 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     7801 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     7861 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     7921 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     7981 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     8041 cccgcaagcc cggtgcctaa atcgatagat cctaatcaac ctctggatta caaaatttgt
     8101 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8161 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8221 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8281 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8341 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8401 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8461 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     8521 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8581 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8641 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     8701 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     8761 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     8821 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8881 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8941 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     9001 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
     9061 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9121 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9181 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9241 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9301 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9361 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9421 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9481 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     9541 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9601 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9661 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9721 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9781 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9841 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9901 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9961 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10021 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10081 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10141 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10201 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10261 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10321 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10381 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10441 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10501 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10561 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10621 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10681 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    10741 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    10801 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10861 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10921 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    10981 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11041 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    11101 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
    11161 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    11221 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
    11281 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    11341 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    11401 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
    11461 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    11521 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    11581 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    11641 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
