LOCUS       sequence-25411-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Firestein R, Bass AJ, Kim SY, Dunn IF, Silver SJ, Guney I, Freed E, 
            Ligon AH, Vena N, Ogino S, Chheda MG, Tamayo P, Finn S, Shrestha Y, 
            Boehm JS, Jain S, Bojarski E, Mermel C, Barretina J, Chan JA, 
            Baselga J, Tabernero J, Root DE, Fuchs CS, Loda M, Shivdasani RA, 
            Meyerson M, Hahn WC
  TITLE     CDK8 is a colorectal cancer oncogene that regulates beta-catenin 
            activity.
  JOURNAL   Nature. 2008 Sep 25. 455(7212):547-51.
  PUBMED    18794900
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2008 Sep 25. 455(7212):547-51."
COMMENT     Sequence Label: pLKO.1.sh.beta-catenin.2279
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        101..611
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     530..548
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(633..652)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tcangaatca actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
       61 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt
      121 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
      181 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
      241 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
      301 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
      361 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
      421 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
      481 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
      541 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
      601 tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
      661 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgant
      721 acccgcaacg cgccanacgt cnatccgnac gcacatcgag cgggtcaccg agctgcanaa
      781 tnttnctcan gccgtcgggt ngaat
//
