LOCUS       sequence-254110-       10107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retrovirus vector to express mCherry-TAZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10107)
  TITLE     Retrovirus vector to express mCherry-TAZ
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQCXIP-mCherry-TAZ
FEATURES             Location/Qualifiers
     source          1..10107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             205..380
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    443..642
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             843..1259
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1189..1211
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1227..1243
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1411..1790
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1791..2002
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1999..2023
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2163..2295
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2278..2297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        2340..2358
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2362..2371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2368..3075
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2513..2531)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2781..2800
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5037..5499
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5093..5110)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5320..5339
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5532..6131
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5532..5551)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6028..6048
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6505..6930
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7033..7055)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7191..7211)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7196..7525
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7376..7511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7547..7566
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7647..7664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7818..8406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7898..7917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8577..9368,9369..9437))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9200..9219
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9438..9542)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9610..9628
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        9728..10107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcaataa aagagcccac aacccctcac tcggcgcgcc
      241 agtcttccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
      301 tgcatccgaa tcgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
      361 cacgacgggg gtctttcatt tgggggctcg tccgggattt ggagacccct gcccagggac
      421 caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg tccgattgtc
      481 tagtgtctat gtttgatgtt atgcgcctgc gtctgtacta gttagctaac tagctctgta
      541 tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac cctgggagac
      601 gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag tcgatgtgga
      661 atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc
      721 ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc
      781 gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt
      841 agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc
      901 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg
      961 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca
     1021 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
     1081 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
     1141 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
     1201 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg
     1261 gaattgaaga tcttcaatat tggccattag ccatattatt cattggttat atagcataaa
     1321 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     1381 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
     1441 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1501 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1561 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1621 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
     1681 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
     1741 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     1801 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     1861 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     1921 gtcgtaacaa ctgcgatcgc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
     1981 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa gctttattgc
     2041 ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa
     2101 cttaagctgc agtgactctc ttaaggtagc cttgcagaag ttggtcgtga ggcactgggc
     2161 aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga
     2221 gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg
     2281 cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc tagagtactt
     2341 aatacgactc actataggct agccaccatg gtgagcaagg gcgaggagga taacatggcc
     2401 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     2461 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     2521 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     2581 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     2641 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     2701 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     2761 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     2821 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     2881 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     2941 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     3001 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     3061 gacgagctgt acaaggaatt cacgcgctca ggctcagctt cgctgcccgc ccagaagatg
     3121 aatccggcct cggcgccccc tccgctcccg ccgcctgggc agcaagtgat ccacgtcacg
     3181 caggacctag acacagacct cgaagccctc ttcaactctg tcatgaatcc gaagcctagc
     3241 tcgtggcgga agaagatcct gccggagtct ttctttaagg agcctgattc gggctcgcac
     3301 tcgcgccagt ccagcaccga ctcgtcgggc ggccacccgg ggcctcgact ggctgggggt
     3361 gcccagcatg tccgctcgca ctcgtcgccc gcgtccctgc agctgggcac cggcgcgggt
     3421 gctgcgggta gccccgcgca gcagcacgcg cacctccgcc agcagtccta cgacgtgacc
     3481 gacgagctgc cactgccccc gggctgggag atgaccttca cggccactgg ccagaggtac
     3541 ttcctcaatc acatagaaaa aatcaccaca tggcaagacc ctaggaaggc gatgaatcag
     3601 cctctgaatc atatgaacct ccaccctgcc gtcagttcca caccagtgcc tcagaggtcc
     3661 atggcagtat cccagccaaa tctcgtgatg aatcaccaac accagcagca gatggccccc
     3721 agtaccctga gccagcagaa ccaccccact cagaacccac ccgcagggct catgagtatg
     3781 cccaatgcgc tgaccactca gcagcagcag cagcagaaac tgcggcttca gagaatccag
     3841 atggagagag aaaggattcg aatgcgccaa gaggagctca tgaggcagga agctgccctc
     3901 tgtcgacagc tccccatgga agctgagact cttgccccag ttcaggctgc tgtcaaccca
     3961 cccacgatga ccccagacat gagatccatc actaataata gctcagatcc tttcctcaat
     4021 ggagggccat atcattcgag ggagcagagc actgacagtg gcctggggtt agggtgctac
     4081 agtgtcccca caactccgga ggacttcctc agcaatgtgg atgagatgga tacaggagaa
     4141 aacgcaggac aaacacccat gaacatcaat ccccaacaga cccgtttccc tgatttcctt
     4201 gactgtcttc caggaacaaa cgttgactta ggaactttgg aatctgaaga cctgatcccc
     4261 ctcttcaatg atgtagagtc tgctctgaac aaaagtgagc cctttctaac ctggctgtaa
     4321 tcactaccat tgtaacttgg atgtagccat gaccttacat ttcctgggcc tcttggaaaa
     4381 agtgatggag cagagcaagt ctgcaggtgc accacttccc gcctccatga ctcgtgctcc
     4441 ctccttttta tgttgccagt ttaatcattg cctggttttg attgagagta acttaagtta
     4501 aacataaata aatattctat tttcattttc tgcaagcctg cgttcttgtg acagattata
     4561 cagaattgtg tctgcaggat tgattatgca gaatactttt ctctttcttc tctgctgccc
     4621 catggctaag ctttatgggt gttaattgaa atttatacac caattgattt taaaccataa
     4681 aaagctgacc acaggcagtt acttctgagg gcatcttggt ccaggaaatg tgcacaaaat
     4741 tcgacctgat ttacagtttc aaaaactgta ttgatgacag tagtaccaaa tgctttaaaa
     4801 actatttaac ttgagcttta aaaatcattg tatggatagt aaaattctac tgtatggaat
     4861 acaatgtaat tttgaatcca aaaaaaaaaa aaaaaaaact cgacccgggc ggccgctcga
     4921 gtttaaacaa ttgcatcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     4981 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     5041 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     5101 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     5161 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     5221 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     5281 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     5341 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     5401 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     5461 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga
     5521 gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5581 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5641 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5701 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5761 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     5821 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5881 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     5941 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6001 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6061 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6121 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     6181 atggctccga ccgaagccac ccggggcggc cccgccgacc ccgcacccgc ccccgaggcc
     6241 caccgactct agtcgagggc tgcagcgctg cagaggccga gtgcagaact gctccaaagg
     6301 gacctcaagg ctttccgagg gacactaggc tgactccatc gagccagtgt agagataagc
     6361 ttatcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac
     6421 tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt
     6481 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     6541 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag
     6601 atcaaggtca ggaacagatg gaacagggtc gaccctagag aaccatcaga tgtttccagg
     6661 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
     6721 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
     6781 tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc
     6841 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     6901 ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct
     6961 gcccagggac caccgaccca ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga
     7021 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     7081 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     7141 cgcagccatg acccagtcac gtagcgatag cggagtgtag atccggctgt ggaatgtgtg
     7201 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7261 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7321 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7381 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7441 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7501 ttttggaggc ctaggctttt gcaaaaagct tactggctta actatgcggc atcagagcag
     7561 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     7621 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7681 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8221 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     8281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8461 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8521 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8581 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8641 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8701 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8761 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8821 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8881 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8941 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9001 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9061 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9121 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9181 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9241 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9301 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9361 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9421 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9481 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9541 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     9601 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattagc ttggccattg
     9661 catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg
     9721 ccatgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     9781 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     9841 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     9901 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     9961 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
    10021 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
    10081 tacgtattag tcatcgctat taccatg
//
