LOCUS       sequence-254111-       10333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas9 plant transcriptional activator (AT-CBF1) linked constitutive 
            expression cassette for Zea mays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Young JK, Gasior SL, Jones S, Wang L, Navarro P, Vickroy B, 
            Barrangou R
  TITLE     The repurposing of type I-E CRISPR-Cascade for gene activation in 
            plants.
  JOURNAL   Commun Biol. 2019 Oct 18;2:383. doi: 10.1038/s42003-019-0637-6. 
            eCollection 2019.
  PUBMED    31646186
REFERENCE   2  (bases 1 to 10333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-10-18"; volume: "2:383. doi"; pages: " 
            10.1038/s42003-019-0637-6. eCollection 2019"
COMMENT     Sequence Label: PV225
FEATURES             Location/Qualifiers
     source          1..10333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     113..135
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     127..144
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     128..144
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     151..170
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        151..169
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        204..2199
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2224..2244
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2620..2808
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     RBS             5994..6002
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(7279..7297)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7280..7297)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7315..7331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7315..7331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7328..7350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7339..7355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7363..7369,7370..7387,7388..7393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7408..7429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7546..7563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7717..8305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7797..7816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(8479..8498)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8490..9305
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             8490..9305
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8563..8582)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9599..9617
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9655..9677)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9777..9796
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9865..10320)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10002..10023)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10214..10233
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
       61 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      121 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt
      181 tacccggacc gaagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag
      241 agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac
      301 ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa
      361 tataatctat agtactacaa taatatcagt gttttagaga atcatataaa tgaacagtta
      421 gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt
      481 agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca
      541 ttttattagt acatccattt agggtttagg gttaatggtt tttatagact aattttttta
      601 gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact ctattttagt
      661 ttttttattt aataatttag atataaaata gaataaaata aagtgactaa aaattaaaca
      721 aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg agtagataat
      781 gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt
      841 cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct
      901 cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg
      961 gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag
     1021 ctacggggga ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa
     1081 tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca
     1141 caaccagatc tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc
     1201 ctcccccccc cccctctcta ccttctctag atcggcgttc cggtccatgc atggttaggg
     1261 cccggtagtt ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc
     1321 tgctagcgtt cgtacacgga tgcgacctgt acgtcagaca cgttctgatt gctaacttgc
     1381 cagtgtttct ctttggggaa tcctgggatg gctctagccg ttccgcagac gggatcgatt
     1441 tcatgatttt ttttgtttcg ttgcataggg tttggtttgc ccttttcctt tatttcaata
     1501 tatgccgtgc acttgtttgt cgggtcatct tttcatgctt ttttttgtct tggttgtgat
     1561 gatgtggtct ggttgggcgg tcgttctaga tcggagtaga attctgtttc aaactacctg
     1621 gtggatttat taattttgga tctgtatgtg tgtgccatac atattcatag ttacgaattg
     1681 aagatgatgg atggaaatat cgatctagga taggtataca tgttgatgcg ggttttactg
     1741 atgcatatac agagatgctt tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg
     1801 tcgttcattc gttctagatc ggagtagaat actgtttcaa actacctggt gtatttatta
     1861 attttggaac tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa gatggatgga
     1921 aatatcgatc taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat
     1981 ggcatatgca gcatctattc atatgctcta accttgagta cctatctatt ataataaaca
     2041 agtatgtttt ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta
     2101 tatgtggatt tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt
     2161 tgtcgatgct caccctgttg tttggtgtta cttctgcagg tcgactctag aggatccatg
     2221 gcaccgaaga agaagcgcaa ggtgatggac aagaagtaca gcatcggcct cgccatcggc
     2281 accaactcgg tgggctgggc cgtcatcacg gacgaatata aggtcccgtc gaagaagttc
     2341 aaggtcctcg gcaatacaga ccgccacagc atcaagaaaa acttgatcgg cgccctcctg
     2401 ttcgatagcg gcgagaccgc ggaggcgacc aggctcaaga ggaccgccag gagacggtac
     2461 actaggcgca agaacaggat ctgctacctg caggagatct tcagcaacga gatggcgaag
     2521 gtggacgact ccttcttcca ccgcctggag gaatcattcc tggtggagga ggacaagaag
     2581 catgagcggc acccaatctt cggcaacatc gtcgacgagg taagtttctg cttctacctt
     2641 tgatatatat ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt
     2701 caaaataaaa gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt
     2761 ttaatttata acttttctaa tatatgacca aaacatggtg atgtgcaggt ggcctaccac
     2821 gagaagtacc cgacaatcta ccacctccgg aagaaactgg tggacagcac agacaaggcg
     2881 gacctccggc tcatctacct tgccctcgcg catatgatca agttccgcgg ccacttcctc
     2941 atcgagggcg acctgaaccc ggacaactcc gacgtggaca agctgttcat ccagctcgtg
     3001 cagacgtaca atcaactgtt cgaggagaac cccataaacg ctagcggcgt ggacgccaag
     3061 gccatcctct cggccaggct ctcgaaatca agaaggctgg agaaccttat cgcgcagttg
     3121 ccaggcgaaa agaagaacgg cctcttcggc aaccttattg cgctcagcct cggcctgacg
     3181 ccgaacttca aatcaaactt cgacctcgcg gaggacgcca agctccagct ctcaaaggac
     3241 acctacgacg acgacctcga caacctcctg gcccagatag gagaccagta cgcggacctc
     3301 ttcctcgccg ccaagaacct ctccgacgct atcctgctca gcgacatcct tcgggtcaac
     3361 accgaaatta ccaaggcacc gctgtccgcc agcatgatta aacgctacga cgagcaccat
     3421 caggacctca cgctgctcaa ggcactcgtc cgccagcagc tccccgagaa gtacaaggag
     3481 atcttcttcg accaatcaaa aaacggctac gcgggatata tcgacggcgg tgccagccag
     3541 gaagagttct acaagttcat caaaccaatc ctggagaaga tggacggcac cgaggagttg
     3601 ctggtcaagc tcaacaggga ggacctcctc aggaagcaga ggaccttcga caacggctcc
     3661 atcccgcatc agatccacct gggcgaactg catgccatcc tgcggcgcca ggaggacttc
     3721 tacccgttcc tgaaggataa ccgggagaag atcgagaaga tcttgacgtt ccgcatccca
     3781 tactacgtgg gcccgctggc tcgcggcaac tcccggttcg cctggatgac ccggaagtcg
     3841 gaggagacca tcacaccctg gaactttgag gaggtggtcg ataagggcgc tagcgctcag
     3901 agcttcatcg agcgcatgac caacttcgat aaaaacctgc ccaatgaaaa agtcctcccc
     3961 aagcactcgc tgctctacga gtacttcacc gtgtacaacg agctcaccaa ggtcaaatac
     4021 gtcaccgagg gcatgcggaa gccggcgttc ctgagcggcg agcagaagaa ggcgatagtg
     4081 gacctcctct tcaagaccaa caggaaggtg accgtgaagc aattaaaaga ggactacttc
     4141 aagaaaatag agtgcttcga ctccgtggag atctcgggcg tggaggatcg gttcaacgcc
     4201 tcactcggca cgtatcacga cctcctcaag atcattaaag acaaggactt cctcgacaac
     4261 gaggagaacg aggacatcct cgaggacatc gtcctcaccc tgaccctgtt cgaggaccgc
     4321 gaaatgatcg aggagaggct gaagacctac gcgcacctgt tcgacgacaa ggtcatgaaa
     4381 cagctcaaga ggcgccgcta cactggttgg ggaaggctgt cccgcaagct cattaatggc
     4441 atcagggaca agcagagcgg caagaccatc ctggacttcc tcaagtccga cgggttcgcc
     4501 aaccgcaact tcatgcagct cattcacgac gactcgctca cgttcaagga agacatccag
     4561 aaggcacagg tgagcgggca gggtgactcc ctccacgaac acatcgccaa cctggccggc
     4621 tcgccggcca ttaaaaaggg catcctgcag acggtcaagg tcgtcgacga gctcgtgaag
     4681 gtgatgggcc ggcacaagcc cgaaaatatc gtcatagaga tggccaggga gaaccagacc
     4741 acccaaaaag ggcagaagaa ctcgcgcgag cggatgaaac ggatcgagga gggcattaaa
     4801 gagctcgggt cccagatcct gaaggagcac cccgtggaaa atacccagct ccagaatgaa
     4861 aagctctacc tctactacct gcagaacggc cgcgacatgt acgtggacca ggagctggac
     4921 attaatcggc tatcggacta cgacgtcgac gccatcgtgc cgcagtcgtt cctcaaggac
     4981 gatagcatcg acaacaaggt gctcacccgg tcggataaaa atcggggcaa gagcgacaac
     5041 gtgcccagcg aggaggtcgt gaagaagatg aaaaactact ggcgccagct cctcaacgcg
     5101 aaactgatca cccagcgcaa gttcgacaac ctgacgaagg cggaacgcgg tggcttgagc
     5161 gaactcgata aggcgggctt cataaaaagg cagctggtcg agacgcgcca gatcacgaag
     5221 catgtcgccc agatcctgga cagccgcatg aatactaagt acgatgaaaa cgacaagctg
     5281 atccgggagg tgaaggtgat cacgctgaag tccaagctcg tgtcggactt ccgcaaggac
     5341 ttccagttct acaaggtccg cgagatcaac aactaccacc acgcccacga cgcctacctg
     5401 aatgcggtgg tcgggaccgc cctgatcaag aagtacccga agctggagtc ggagttcgtg
     5461 tacggcgact acaaggtcta cgacgtgcgc aaaatgatcg ccaagtccga gcaggagatc
     5521 ggcaaggcca cggcaaaata cttcttctac tcgaacatca tgaacttctt caagaccgag
     5581 atcaccctcg cgaacggcga gatccgcaag cgcccgctca tcgaaaccaa cggcgagacg
     5641 ggcgagatcg tctgggataa gggccgggat ttcgcgacgg tccgcaaggt gctctccatg
     5701 ccgcaagtca atatcgtgaa aaagacggag gtccagacgg gcgggttcag caaggagtcc
     5761 atcctcccga agcgcaactc cgacaagctc atcgcgagga agaaggattg ggacccgaaa
     5821 aaatatggcg gcttcgacag cccgaccgtc gcatacagcg tcctcgtcgt ggcgaaggtg
     5881 gagaagggca agtcaaagaa gctcaagtcc gtgaaggagc tgctcgggat cacgattatg
     5941 gagcggtcct ccttcgagaa gaacccgatc gacttcctag aggccaaggg atataaggag
     6001 gtcaagaagg acctgattat taaactgccg aagtactcgc tcttcgagct ggaaaacggc
     6061 cgcaagagga tgctcgcctc cgcaggcgag ttgcagaagg gcaacgagct cgccctcccg
     6121 agcaaatacg tcaatttcct gtacctcgct agccactatg aaaagctcaa gggcagcccg
     6181 gaggacaacg agcagaagca gctcttcgtg gagcagcaca agcattacct ggacgagatc
     6241 atcgagcaga tcagcgagtt ctcgaagcgg gtgatcctcg ccgacgcgaa cctggacaag
     6301 gtgctgtcgg catataacaa gcaccgcgac aaaccaatac gcgagcaggc cgaaaatatc
     6361 atccacctct tcaccctcac caacctcggc gctccggcag ccttcaagta cttcgacacc
     6421 acgattgacc ggaagcggta cacgagcacg aaggaggtgc tcgatgcgac gctgatccac
     6481 cagagcatca cagggctcta tgaaacacgc atcgacctga gccagctggg cggagacaag
     6541 agaccacggg accgccacga tggcgagctg ggaggccgca agcgggcaag gggcagggct
     6601 acgtgcgcca aggacatcca aaaagcagcc gcggaggcgg cactcgcctt tcaggacgag
     6661 acctgcgaca ccaccaccac caaccacggc ctcgacatgg aggagaccat ggtcgaagcc
     6721 atctacaccc ccgagcagag cgagggcgcc ttctacatgg acgaagagac aatgttcggc
     6781 atgccaaccc tcctcgacaa catggccgaa ggcatgctgc tgccgccccc gagcgtccag
     6841 tggaaccaca actacgacgg cgaaggcgac ggggatgtgt ccctgtggag ctactaggta
     6901 ccgttaacct agacttgtcc atcttctgga ttggccaact taattaatgt atgaaataaa
     6961 aggatgcaca catagtgaca tgctaatcac tataatgtgg gcatcaaagt tgtgtgttat
     7021 gtgtaattac tagttatctg aataaaagag aaagagatca tccatatttc ttatcctaaa
     7081 tgaatgtcac gtgtctttat aattctttga tgaaccagat gcatttcatt aaccaaatcc
     7141 atatacatat aaatattaat catatataat taatatcaat tgggttagca aaacaaatct
     7201 agtctaggtg tgttttgcga atgcggccgc caccgcggtg gagctcgaat tccggtccgg
     7261 gtcacccagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
     7321 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7381 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7441 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7501 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc gataggctcc gcccccctga
     7741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8341 tcagtggaac gaaaactcac gttaagggat tttggtcatg gagccacgtt gtgtctcaaa
     8401 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     8461 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct
     8521 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     8581 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
     8641 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     8701 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     8761 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
     8821 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     8881 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     8941 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     9001 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     9061 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     9121 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     9181 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcagaaat
     9241 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     9301 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
     9361 acggcggctt tgttgaataa atcgaacttt tgctgacttg aaggatcaga tcacgcatct
     9421 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac
     9481 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat
     9541 tcaggcctgg tatgagtcag caacaccttc ttcacgagcc atgacattaa cctataaaaa
     9601 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     9661 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     9721 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     9781 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     9841 aaggagaaaa taccgcatca ggcgaaattg taaacgttaa tattttgtta aaattcgcgt
     9901 taaatatttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     9961 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
    10021 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
    10081 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc
    10141 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
    10201 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
    10261 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
    10321 ccattcgcca ttc
//
