LOCUS       sequence-254148-       11703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CB R604Pfs*29.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CB-R604Pfs*29
FEATURES             Location/Qualifiers
     source          1..11703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             3656..3664
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4963..4974)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6663..6687)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6695..6713
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6738..6757)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6739..6757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6763..6783)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6837..6878
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6899..7466
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6916..7468
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7060..7077)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7287..7306
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7480..8079
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7480..7499)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7976..7996
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8093..8681
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8146..8166)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8564..8575)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8756..8989
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9108..9126)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9194..9298
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9299..9367,9368..10159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9517..9536)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10330..10918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10819..10838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11072..11089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11329..11350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11365..11370,11371..11388,11389..11395)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11403..11419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11408..11430
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11427..11443
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11427..11443
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11493..11512
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg tgcttcagtt tcataatgcc tcctgctatg
     3481 gcagacatcc ttgacatctg ggcggtggat tcacagatag catctgatgg ctccatacct
     3541 gtggatttcc ttttgcccac tgggatttat atccagttgg aggtacctcg ggaagctacc
     3601 atttcttata ttaagcagat gttatggaag caagttcaca attacccaat gttcaacctc
     3661 cttatggata ttgactccta tatgtttgca tgtgtgaatc agactgctgt atatgaggag
     3721 cttgaagatg aaacacgaag actctgtgat gtcagacctt ttcttccagt tctcaaatta
     3781 gtgacaagaa gttgtgaccc aggggaaaaa ttagactcaa aaattggagt ccttatagga
     3841 aaaggtctgc atgaatttga ttccttgaag gatcctgaag taaatgaatt tcgaagaaaa
     3901 atgcgcaaat tcagcgagga aaaaatcctg tcacttgtgg gattgtcttg gatggactgg
     3961 ctaaaacaaa catatccacc agagcatgaa ccatccatcc ctgaaaactt agaagataaa
     4021 ctttatgggg gaaagctcat cgtagctgtt cattttgaaa actgccagga cgtgtttagc
     4081 tttcaagtgt ctcctaatat gaatcctatc aaagtaaatg aattggcaat ccaaaaacgt
     4141 ttgactattc atgggaagga agatgaagtt agcccctatg attatgtgtt gcaagtcagc
     4201 gggagagtag aatatgtttt tggtgatcat ccactaattc agttccagta tatccggaac
     4261 tgtgtgatga acagagccct gccccatttt atacttgtgg aatgctgcaa gatcaagaaa
     4321 atgtatgaac aagaaatgat tgccatagag gctgccataa atcgaaattc atctaatctt
     4381 cctcttccat taccaccaaa gaaaacacga attatttctc atgtttggga aaataacaac
     4441 cctttccaaa ttgtcttggt taagggaaat aaacttaaca cagaggaaac tgtaaaagtt
     4501 catgtcaggg ctggtctttt tcatggtact gagctcctgt gtaaaaccat cgtaagctca
     4561 gaggtatcag ggaaaaatga tcatatttgg aatgaaccac tggaatttga tattaatatt
     4621 tgtgacttac caagaatggc tcgattatgt tttgctgttt atgcagtttt ggataaagta
     4681 aaaacgaaga aatcaacgaa aactattaat ccctctaaat atcagaccat caggaaagct
     4741 ggaaaagtgc attatcctgt agcgtgggta aatacgatgg tttttgactt taaaggacaa
     4801 ttgagaactg gagacataat attacacagc tggtcttcat ttcctgatga actcgaagaa
     4861 atgttgaatc caatgggaac tgttcaaaca aatccatata ctgaaaatgc aacagctttg
     4921 catgttaaat ttccagagaa taaaaaacaa ccttattatt accctccctt cgataagatt
     4981 attgaaaagg cagctgagat tgcaagcagt gatagtgcta atgtgtcaag tcgaggtgga
     5041 aaaaagtttc ttcctgtatt gaaagaaatc ttggacaggg atcccttgtc tcaactgtgt
     5101 gaaaatgaaa tggatcttat ttggactttg cgacaagact gccgagagat tttcccacaa
     5161 tcactgccaa aattactgct gtcaatcaag tggaataaac ttgaggatgt tgctcagctt
     5221 caggcgctgc ttcagatttg gcctaaactg ccccccccgg gaggccctag agcttctgga
     5281 tttcaactat ccagaccagt acgttcgaga atatgctgta ggctgcctgc gacagatgag
     5341 tgatgaagaa ctttctcaat atcttttaca actggtgcaa gtgttaaaat atgagccttt
     5401 tcttgattgt gccctctcta gattcctatt agaaagagca cttggtaatc ggaggatagg
     5461 gcagtttcta ttttggcatc ttaggtcaga agtgcacatt cctgctgtct cagtacaatt
     5521 tggtgtcatc cttgaagcat actgccgggg aagtgtgggg cacatgaaag tgctttctaa
     5581 gcaggttgaa gcactcaata agttaaaaac tttaaatagt ttaatcaaac tgaatgccgt
     5641 gaagttaaac agagccaaag ggaaggaggc catgcatacc tgtttaaaac agagtgctta
     5701 ccgggaagcc ctctctgacc tgcagtcacc cctgaaccca tgtgttatcc tctcagaact
     5761 ctatgttgaa aagtgcaaat acatggattc caaaatgaag cctttgtggc tggtatacaa
     5821 taacaaggta tttggtgagg attcagttgg agtgattttt aaaaatggtg atgatttacg
     5881 acaggatatg ttgacactcc aaatgttgcg cttgatggat ttactctgga aagaagctgg
     5941 tttggatctt cggatgttgc cttatggctg tttagcaaca ggagatcgct ctggcctcat
     6001 tgaagttgtg agcacctctg aaacaattgc tgacattcag ctgaacagta gcaatgtggc
     6061 tgctgcagca gccttcaaca aagatgccct tctgaactgg cttaaagaat acaactctgg
     6121 ggatgacctg gaccgagcca ttgaggaatt tacactgtcc tgtgctggct actgtgtagc
     6181 ttcttatgtc cttgggattg gtgacagaca tagtgacaac atcatggtca aaaaaactgg
     6241 ccagctcttc cacattgact ttggacatat tcttggaaat ttcaaatcta agtttggcat
     6301 taaaagggag cgagtgcctt ttattcttac ctatgatttc atccatgtca ttcaacaagg
     6361 aaaaacagga aatacagaaa agtttggccg gttccgccag tgttgtgagg atgcatatct
     6421 gattttacga cggcatggga atctcttcat cactctcttt gcgctgatgt tgactgcagg
     6481 gcttcctgaa ctcacatcag tcaaagatat acagtatctt aaggactctc ttgcattagg
     6541 gaagagtgaa gaagaagcac tcaaacagtt taagcaaaaa tttgatgagg cgctcaggga
     6601 aagctggact actaaagtga actggatggc ccacacagtt cggaaagact acagatctta
     6661 gaacccagct ttcttgtaca aagtggtgat atccaattaa ccctcactaa aggaaatgtg
     6721 tgtttagtag ttgtaaaccc tatagtgagt cgtattacca cccaactttt ctatacaaag
     6781 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta
     6841 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag taatgagatc
     6901 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     6961 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     7021 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     7081 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     7141 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     7201 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     7261 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     7321 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     7381 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     7441 ttttcctttg aaaaacacga tgataatatg gccacacata tgaccgagta caagcccacg
     7501 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     7561 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     7621 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     7681 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     7741 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     7801 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     7861 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     7921 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     7981 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8041 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaaa tcgatagatc ctaatcaacc
     8101 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     8161 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     8221 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     8281 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     8341 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     8401 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     8461 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     8521 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     8581 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     8641 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca
     8701 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8761 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     8821 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8881 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8941 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     9001 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     9061 cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg
     9121 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     9181 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9241 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     9301 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     9361 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     9421 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     9481 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     9541 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     9601 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     9661 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     9721 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9781 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9841 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9901 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9961 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    10021 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    10081 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    10141 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    10201 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    10261 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    10321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    10381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    10441 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    10501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    10561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    10621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    10981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    11041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    11101 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct
    11161 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    11221 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg
    11281 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    11341 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    11401 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac
    11461 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11521 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    11581 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    11641 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    11701 agt
//
