LOCUS       sequence-254150-       11701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CB R604Gfs*4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11701)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CB-R604Gfs*4
FEATURES             Location/Qualifiers
     source          1..11701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             3656..3664
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4963..4974)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6661..6685)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6693..6711
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6736..6755)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6737..6755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6761..6781)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6835..6876
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6897..7464
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6914..7466
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7058..7075)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7285..7304
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7478..8077
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7478..7497)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7974..7994
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8091..8679
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8144..8164)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8562..8573)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8754..8987
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9106..9124)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9192..9296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9297..9365,9366..10157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9515..9534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10328..10916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10817..10836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11070..11087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11327..11348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11363..11368,11369..11386,11387..11393)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11401..11417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11406..11428
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11425..11441
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11425..11441
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11491..11510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg tgcttcagtt tcataatgcc tcctgctatg
     3481 gcagacatcc ttgacatctg ggcggtggat tcacagatag catctgatgg ctccatacct
     3541 gtggatttcc ttttgcccac tgggatttat atccagttgg aggtacctcg ggaagctacc
     3601 atttcttata ttaagcagat gttatggaag caagttcaca attacccaat gttcaacctc
     3661 cttatggata ttgactccta tatgtttgca tgtgtgaatc agactgctgt atatgaggag
     3721 cttgaagatg aaacacgaag actctgtgat gtcagacctt ttcttccagt tctcaaatta
     3781 gtgacaagaa gttgtgaccc aggggaaaaa ttagactcaa aaattggagt ccttatagga
     3841 aaaggtctgc atgaatttga ttccttgaag gatcctgaag taaatgaatt tcgaagaaaa
     3901 atgcgcaaat tcagcgagga aaaaatcctg tcacttgtgg gattgtcttg gatggactgg
     3961 ctaaaacaaa catatccacc agagcatgaa ccatccatcc ctgaaaactt agaagataaa
     4021 ctttatgggg gaaagctcat cgtagctgtt cattttgaaa actgccagga cgtgtttagc
     4081 tttcaagtgt ctcctaatat gaatcctatc aaagtaaatg aattggcaat ccaaaaacgt
     4141 ttgactattc atgggaagga agatgaagtt agcccctatg attatgtgtt gcaagtcagc
     4201 gggagagtag aatatgtttt tggtgatcat ccactaattc agttccagta tatccggaac
     4261 tgtgtgatga acagagccct gccccatttt atacttgtgg aatgctgcaa gatcaagaaa
     4321 atgtatgaac aagaaatgat tgccatagag gctgccataa atcgaaattc atctaatctt
     4381 cctcttccat taccaccaaa gaaaacacga attatttctc atgtttggga aaataacaac
     4441 cctttccaaa ttgtcttggt taagggaaat aaacttaaca cagaggaaac tgtaaaagtt
     4501 catgtcaggg ctggtctttt tcatggtact gagctcctgt gtaaaaccat cgtaagctca
     4561 gaggtatcag ggaaaaatga tcatatttgg aatgaaccac tggaatttga tattaatatt
     4621 tgtgacttac caagaatggc tcgattatgt tttgctgttt atgcagtttt ggataaagta
     4681 aaaacgaaga aatcaacgaa aactattaat ccctctaaat atcagaccat caggaaagct
     4741 ggaaaagtgc attatcctgt agcgtgggta aatacgatgg tttttgactt taaaggacaa
     4801 ttgagaactg gagacataat attacacagc tggtcttcat ttcctgatga actcgaagaa
     4861 atgttgaatc caatgggaac tgttcaaaca aatccatata ctgaaaatgc aacagctttg
     4921 catgttaaat ttccagagaa taaaaaacaa ccttattatt accctccctt cgataagatt
     4981 attgaaaagg cagctgagat tgcaagcagt gatagtgcta atgtgtcaag tcgaggtgga
     5041 aaaaagtttc ttcctgtatt gaaagaaatc ttggacaggg atcccttgtc tcaactgtgt
     5101 gaaaatgaaa tggatcttat ttggactttg cgacaagact gccgagagat tttcccacaa
     5161 tcactgccaa aattactgct gtcaatcaag tggaataaac ttgaggatgt tgctcagctt
     5221 caggcgctgc ttcagatttg gcctaaactg ccccccggga ggccctagag cttctggatt
     5281 tcaactatcc agaccagtac gttcgagaat atgctgtagg ctgcctgcga cagatgagtg
     5341 atgaagaact ttctcaatat cttttacaac tggtgcaagt gttaaaatat gagccttttc
     5401 ttgattgtgc cctctctaga ttcctattag aaagagcact tggtaatcgg aggatagggc
     5461 agtttctatt ttggcatctt aggtcagaag tgcacattcc tgctgtctca gtacaatttg
     5521 gtgtcatcct tgaagcatac tgccggggaa gtgtggggca catgaaagtg ctttctaagc
     5581 aggttgaagc actcaataag ttaaaaactt taaatagttt aatcaaactg aatgccgtga
     5641 agttaaacag agccaaaggg aaggaggcca tgcatacctg tttaaaacag agtgcttacc
     5701 gggaagccct ctctgacctg cagtcacccc tgaacccatg tgttatcctc tcagaactct
     5761 atgttgaaaa gtgcaaatac atggattcca aaatgaagcc tttgtggctg gtatacaata
     5821 acaaggtatt tggtgaggat tcagttggag tgatttttaa aaatggtgat gatttacgac
     5881 aggatatgtt gacactccaa atgttgcgct tgatggattt actctggaaa gaagctggtt
     5941 tggatcttcg gatgttgcct tatggctgtt tagcaacagg agatcgctct ggcctcattg
     6001 aagttgtgag cacctctgaa acaattgctg acattcagct gaacagtagc aatgtggctg
     6061 ctgcagcagc cttcaacaaa gatgcccttc tgaactggct taaagaatac aactctgggg
     6121 atgacctgga ccgagccatt gaggaattta cactgtcctg tgctggctac tgtgtagctt
     6181 cttatgtcct tgggattggt gacagacata gtgacaacat catggtcaaa aaaactggcc
     6241 agctcttcca cattgacttt ggacatattc ttggaaattt caaatctaag tttggcatta
     6301 aaagggagcg agtgcctttt attcttacct atgatttcat ccatgtcatt caacaaggaa
     6361 aaacaggaaa tacagaaaag tttggccggt tccgccagtg ttgtgaggat gcatatctga
     6421 ttttacgacg gcatgggaat ctcttcatca ctctctttgc gctgatgttg actgcagggc
     6481 ttcctgaact cacatcagtc aaagatatac agtatcttaa ggactctctt gcattaggga
     6541 agagtgaaga agaagcactc aaacagttta agcaaaaatt tgatgaggcg ctcagggaaa
     6601 gctggactac taaagtgaac tggatggccc acacagttcg gaaagactac agatcttaga
     6661 acccagcttt cttgtacaaa gtggtgatat ccaattaacc ctcactaaag gaagatgata
     6721 gttaagtgta agttacccta tagtgagtcg tattaccacc caacttttct atacaaagtg
     6781 gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
     6841 cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc
     6901 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     6961 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     7021 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     7081 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     7141 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     7201 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     7261 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     7321 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     7381 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     7441 ttcctttgaa aaacacgatg ataatatggc cacacatatg accgagtaca agcccacggt
     7501 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     7561 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     7621 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     7681 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     7741 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     7801 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     7861 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     7921 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     7981 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     8041 gcgcacctgg tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct aatcaacctc
     8101 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     8161 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     8221 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     8281 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     8341 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     8401 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     8461 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     8521 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     8581 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     8641 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat
     8701 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8761 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     8821 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8881 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8941 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     9001 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     9061 cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc
     9121 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     9181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     9541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9601 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     9661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     9721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     9781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     9841 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9901 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9961 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10021 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10081 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10141 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    10381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    10441 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    10501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    10561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    10621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    10681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    10741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    11101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca
    11161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    11221 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc
    11281 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    11341 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    11401 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa
    11461 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    11521 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    11581 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    11641 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    11701 t
//
