LOCUS       sequence-254156-       11017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 12xgp41 peptide array (MoonTag peptide array), followed by
            12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 
            repeats of PP7 hairpins in 3' UTR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Boersma S, Khuperkar D, Verhagen BMP, Sonneveld S, Grimm JB, Lavis 
            LD, Tanenbaum ME
  TITLE     Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity
            in mRNA Decoding.
  JOURNAL   Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi: 
            10.1016/j.cell.2019.05.001.
  PUBMED    31178119
REFERENCE   2  (bases 1 to 11017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 5. pii: S0092-8674(19)30499-4. doi: 10.1016/j.cell.2019.05.001."
COMMENT     Sequence Label: 12xMoonTag-12xSunTag-kif18b-24xPP7
FEATURES             Location/Qualifiers
     source          1..11017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1029..1073
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1107..1151
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1185..1229
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1263..1307
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1344..1388
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1425..1469
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1506..1550
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1587..1631
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1668..1712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1749..1793
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1827..1871
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1908..1952
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1992..2048
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2064..2120
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2136..2192
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2208..2264
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2280..2336
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2352..2408
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2424..2480
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2496..2552
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2568..2624
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2640..2696
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2712..2768
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2784..2840
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(7025..7042)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7031..7255
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7301..7729
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7388..7407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7598..7619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7738..7758)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7743..8072
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7923..8058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7985..8004
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8120..8167
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8186..8560
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8690..8811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8727..8746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8781..8800
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8860..8876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8860..8876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8873..8895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8884..8900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8908..8914,8915..8932,8933..8938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8953..8974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9091..9108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9262..9850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9342..9361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10021..10812,10813..10881))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10644..10663
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10882..10986)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
     1021 tcggtccgaa gaacgagcag gaactgctgg agctggacaa atgggcctct ctgggcgggg
     1081 gaagcggagg gggctccgga ggaggaaaaa acgaacagga gctgctggag ctggataaat
     1141 gggcctccct gggcggaggg tccggaggag ggtccggggg cggaaaaaat gaacaggaac
     1201 tgctggagct ggacaagtgg gcaagcctgg gggggggcag cggcggaggc tccggaggcg
     1261 gaaaaaatga acaggagctg ctggagctgg ataagtgggc ttctctgggg agcggcgggt
     1321 ccggagccgg gagcgctggg tctaagaacg agcaggaact gctggagctg gacaaatggg
     1381 cctctctggg cagcggcggg tccggagctg gatctgccgg ctctaaaaac gaacaggagc
     1441 tgctggagct ggataaatgg gcctccctgg gctctggcgg cagcggcgcc gggtctgccg
     1501 ggtctaaaaa tgaacaggaa ctgctggagc tggacaagtg ggcaagcctg ggagggtctg
     1561 cagggggatc cggagcaagc ggaggaaaaa atgaacagga gctgctggag ctggataagt
     1621 gggcttctct gggcggatcc gctggggggt ccggagcttc tggcggcaag aacgagcagg
     1681 aactgctgga gctggacaaa tgggcctctc tggggggatc cgcaggaggg tctggcgctt
     1741 ccggcggcaa aaacgaacag gagctgctgg agctggataa atgggcctcc ctgggaggag
     1801 gatctggagg agggtctggg ggaggaaaaa atgaacagga actgctggag ctggacaagt
     1861 gggcaagcct gggctctggc ggcagcggcg ccgggtctgc cgggtctaaa aatgaacagg
     1921 agctgctgga gctggataag tgggcttctc tgccgcggga ccggtggcgt acgggcacag
     1981 aattcagcgg cgaagaatta ctgtcaaaaa attaccatct ggaaaacgaa gtggcccggc
     2041 tcaaaaaagg aagcgggtcc ggggaagaac tcttatccaa gaattatcac ttggaaaacg
     2101 aggtggctcg cctcaaaaaa ggctccgggt ctggcgaaga actgctcagc aaaaactatc
     2161 atctcgagaa cgaggtggca aggctcaaaa aaggaggttc gagcggagaa gagcttctat
     2221 ccaagaacta tcacctcgag aacgaggtcg ccagactcaa aaagtctgga agcggttcgg
     2281 aagagttgct ttcgaaaaat tatcacctgg aaaacgaggt cgctcgactc aaaaagggtt
     2341 caagcggagg cgaagagcta ctcagcaaga attatcatct cgaaaacgaa gtcgcacgtc
     2401 tcaaaaaggg ttctggaggg ggggaggaac tgctatcaaa aaactatcac ttggagaacg
     2461 aggttgcccg gctcaagaaa ggctctggtt caggagagga actcctgtcg aagaactacc
     2521 atttggagaa cgaagttgct cgcctcaaga aaggctctgg ttcaggagag gaattactca
     2581 gcaaaaatta ccatctggaa aacgaggttg cacgactcaa gaaaggaggg tccggcggag
     2641 aggagcttct ttcgaagaat taccacttgg aaaacgaagt ggcgagactc aagaagggtt
     2701 ctggaagtgg ggaggagttg ttatccaaaa actaccacct ggagaacgaa gtcgccaggc
     2761 tcaagaagag cggcagcggc agcgaggagc tgctgtccaa gaactaccat ctcgagaacg
     2821 aagttgcgcg tctcaagaag catcgagcgc gcaccggtgc agtggaggac agcacgctgc
     2881 aagtagtggt acgggtgcgg ccccccaccc ctcgggagct ggacagtcag cggcggccag
     2941 tggttcaggt ggtggacgag cgggtgctgg tgtttaaccc tgaggagccc gatggagggt
     3001 tccctggcct gaaatggggt ggcacccatg atggccccaa gaagaagggc aaagacctga
     3061 cgtttgtctt tgaccgggtc tttggcgagg cggccaccca acaggacgtg ttccagcaca
     3121 ccacgcacag cgtcctggac agcttcctcc agggctacaa ctgctcagtg tttgcctacg
     3181 gggccaccgg ggctgggaag acacacacca tgctgggaag ggagggggac cccggcatca
     3241 tgtacctgac caccgtggaa ctgtacaggc gcctggaggc ccgccagcag gagaagcact
     3301 tcgaggtgct catcagctac caggaggtgt ataatgaaca gatccatgac ctcctggagc
     3361 ccaaggggcc ccttgccatc cgcgaggacc ccgacaaggg ggtggtggtg caaggacttt
     3421 ctttccacca gccagcctca gccgagcagc tgctggagat actgaccagg gggaaccgta
     3481 accgcacgca gcaccccact gatgccaacg cgacttcctc ccgctcccat gccatcttcc
     3541 agatctttgt gaagcagcag gaccgggttc caggactgac ccaggctgtc caggtggcca
     3601 agatgagcct gattgacctg gctggctcag agcgggcatc cagcacccat gcgaaggggg
     3661 agcggctgcg ggagggggcc aacatcaacc gctctctgct ggcgctcatc aacgtcctca
     3721 atgccttggc cgatgcaaag ggccgcaaga ccgctgtgcc ctacgcggac agcgcactga
     3781 cccgcctgct caaagactcc ctcgggggca actgccgcac agtgatgatc gctgccatca
     3841 gcccctccag cctgacctac gaggacacgt ataataccct caaatatgcc gaccgggcca
     3901 aggagatcag gctctcgctg aagagcaatg tgaccagcct ggactgtcac atcagccagt
     3961 atgctaccat ctgccaacag ctccaggctg aggtagccgc tctgaggaag aagctccaag
     4021 tgtatgaggg gggaggccag cccccaccac aggacctccc aggatctccc aagtcgggac
     4081 caccaccaga acaccttccc agctccccct tgccacccca ccctcccagc cagccctgca
     4141 ccccagagct ccctgcaggg cctagagccc ttcaagagga gagtctgggg atggaggccc
     4201 aggtggagag ggccatggaa gggaactctt cagaccagga gcagtcccca gaggatgagg
     4261 atgaaggccc agctgaggag gttccaaccc agatgccaga gcagaacccc acacatgcac
     4321 tgccagagtc ccctcgcctg accctgcagc ccaagccagt cgtgggccac ttctcagcac
     4381 gggaactgga tggggaccgt tctaagcggt tggccctaaa ggtgctgtgc gttgcccagc
     4441 ggcagtactc cctgctccaa gcagccaacc tcctgacgcc cgacatgatc acagagtttg
     4501 agaccctaca gcagctggtg caagaggaaa aaattgagcc tggggcagag gccttgagga
     4561 cttcaggcct ggccaggggg gcacctctgg ctcaggagct gtgttcagag tcaaagcctc
     4621 caggatacac tggccctgtg acccggacta tggcgaggcg actgagtggc cccctgcaca
     4681 ccctgggaat cccgcctgga cccaactgca ccccagccca ggggtcccga tggcccatgg
     4741 agaagaagag gaggagacca agcgccttgg aggcagacag tcccatggcc ccaaagcggg
     4801 gcaccaagcg ccagcgccag tccttcctgc cctgcctaag gagagggtct ctgcctgaca
     4861 cccaaccttc acaggggccc agcaccccca aaggagaaag ggcctcctcc ccctgccatt
     4921 cccctcgcgt ttgcccagcc acagtcatca aaagccgggt gcccctgggc ccttccgcca
     4981 tgcagaactg ctccaccccg ctggctctgc ccactcgaga cctcaatgcc acctttgatc
     5041 tctctgagga gcctccctca aagcccagtt tccatgaatg cattggctgg gacaaaatac
     5101 cccaggagct gagcaggctg gaccagccct tcatccccag ggcacctgtg cccctgttca
     5161 ccatgaaggg ccccaagcca acatcttccc tccctgggac ctctgcctgc aagaagaagc
     5221 gcgttgcgag ttcctcagtc tcccatggcc gcagccgcat cgcccgcctc cccagcagca
     5281 ctttgaagag gccagctggg ccccttgtac tcccagagct gcccttgagt cccctgtgcc
     5341 ctagcaaccg gaggaatgga aaggacctca tcagggtggg gagagcactc tcagcaggga
     5401 acggcgtcac caaggtgtcc gatatctaac gacacgattc gaagatcgca tcgctagccc
     5461 ggttgtacag gtaccggatc ctaaggtacc taattgccta gaaaggagca gacgatatgg
     5521 cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg ctggctgcag
     5581 tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc agacgatatg
     5641 gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc gctggctgca
     5701 gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag cagacgatat
     5761 ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct cgctggctgc
     5821 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga gcagacgata
     5881 tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc tcgctggctg
     5941 cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg agcagacgat
     6001 atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg ctcgctggct
     6061 gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag gagcagacga
     6121 tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg gctcgctggc
     6181 tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa ggagcagacg
     6241 atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg
     6301 ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac
     6361 gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg
     6421 gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga
     6481 cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct
     6541 ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag
     6601 acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc
     6661 tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca
     6721 gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg
     6781 ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc
     6841 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc
     6901 gctggctgca gtattcccgg gttcattaga tctcgcgaag ggcgaattca cgaggcgcgc
     6961 ccggatcgcg atcgcaatct taattaactc gagtctagag ggcccgttta aacccgctga
     7021 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7081 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7141 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     7201 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     7261 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     7321 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7381 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7441 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7501 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7561 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7621 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7681 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     7741 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7801 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7861 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7921 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7981 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     8041 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     8101 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     8161 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     8221 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     8281 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     8341 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     8401 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     8461 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     8521 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     8581 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     8641 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     8701 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8761 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8821 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8881 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8941 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9001 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9061 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9121 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9181 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9241 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9301 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9361 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9421 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9481 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9601 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9661 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9721 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9781 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9841 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9901 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9961 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10021 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10081 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10141 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10201 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10261 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10321 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10381 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10441 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10501 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10561 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10621 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10681 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10741 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10801 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10861 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10921 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10981 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
