LOCUS       sequence-254159-       11791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Sec16A as a GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Watson P, Townley AK, Koka P, Palmer KJ, Stephens DJ
  TITLE     Sec16 defines endoplasmic reticulum exit sites and is required for 
            secretory cargo export in mammalian cells.
  JOURNAL   Traffic. 2006 Dec;7(12):1678-87. Epub 2006 Sep 27.
  PUBMED    17005010
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic."; 
            date: "2006-12"; volume: "7(12)"; pages: "1678-87. Epub 2006 Sep 27"
COMMENT     Sequence Label: pEGFP-Sec16A
FEATURES             Location/Qualifiers
     source          1..11791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8579..8700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8616..8635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8670..8689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8707..9162)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8844..8865)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9056..9075
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9189..9293
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9295..9652
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9295..9486
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9318..9338)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9503..9638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9687..10481
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9687..10481
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9741..9760)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10351..10370
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10669..10688)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10713..10760
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11089..11677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11578..11597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctatgcag ccaccgcccc agacggtccc gtctggcatg
     1381 gctgggccac ctccagccgg gaatcctcgg agcgtgttct gggctagcag cccttacagg
     1441 agacgggcta ataataatgc agcagtggct ccgacaactt gcccgttgca gccggtcacg
     1501 gatccatttg cttttagtag acaggcgctc caaagtacac cactgggcag ttcgtccaaa
     1561 agcagtccac ctgtcttgca aggcccagcc cccgcagggt tttctcagca ccccggtttg
     1621 cttgttcctc acacacatgc cagagatagc tctcagggac cctgtgagcc cctgcctgga
     1681 cctctgacac agcccagagc acatgccagt ccgttttctg gtgcattgac accttcagca
     1741 cctcctgggc ctgagatgaa caggagtgca gaggtcggtc ccagttcaga gcctgaagtt
     1801 cagactctgc catatcttcc tcactacatt ccaggagtgg atcctgaaac gtctcatggg
     1861 ggccaccctc atgggaacat gcctgggctc gaccgacccc tgagcaggca aaacccacat
     1921 gacggtgtgg tcaccccagc agcatcccct tccctccctc agcctggtct gcagatgcca
     1981 ggacagtggg ggccagtgca gggaggccca cagccctcgg ggcaacatcg ttcaccctgc
     2041 cctgaaggac ctgttcccag cggggtgccc tgtgccacca gcgttcctca tttccccacc
     2101 ccgtccatcc tacatcaggg ccctggtcat gagcaacaca gccctctggt ggctccccca
     2161 gcagccttgc ccagtgacgg aagagacgag gtgagccact tgcaaagtgg aagccacctg
     2221 gccaataact ctgatcctga aagtacattc aggcaaaatc ccagaattgt gaatcactgg
     2281 gcaagcccag agctcaggca gaatccagga gtgaagaatg agcaccggcc cgcctctgct
     2341 cttgtgaacc ccctcgcccg gggagatagc ccagaaaacc gtacgcacca cccactgggg
     2401 gctggggccg ggtctggctg tgccccgcta gaagcagact caggagcttc aggagctctg
     2461 gcgatgtttt tccaaggggg agagacagaa aatgaggaga atctctcatc tgaaaaagca
     2521 ggcttatctg gtcaagcgga ctttgacgat ttctgctcca gccctgggct aggccgtccg
     2581 cccgcaccta cacacgtggg ggcaggcagc ctctgccagg cccttctccc aggccccagc
     2641 aatgaggctg ctggtgatgt gtggggtgac acagcgagca caggggtgcc ggatgccagc
     2701 ggctcgcagt atgagaatgt tgagaactta gaatttgttc agaatcaaga agttctgcca
     2761 agtgagcccc tcaatttgga cccttcctcc ccgagtgacc agttcagata tgggcccctt
     2821 cctgggccag ctgtgcccag gcatggtgct gtgtgccaca ccggagcccc tgatgccaca
     2881 ctgcatacag tgcaccctga cagcgtgtca tccagctata gcagcagaag ccacggaagg
     2941 ctctcaggct cagccaggcc ccaggagctg gttggcacat tcattcagca agaagttgga
     3001 aaacccgagg atgaagcttc aggtagtttt tttaagcaaa tcgattcttc tcccgtagga
     3061 ggtgaaacag acgagaccac tgtgagccag aattaccgtg gcagcgtgtc ccagccctca
     3121 accccgagcc ccccgaaacc tacaggaata tttcagacaa gtgcaaatag ttctttcgaa
     3181 ccggtaaaat ctcacttagt tggggtaaaa ccatttgagg cagatcgcgc caacgtggtt
     3241 ggtgaagtaa gggagacctg tgtccgccag aagcagtgca gaccagctgc cgccctgccc
     3301 gatgcttccc ctggcaacct ggagcagcca ccagacaaca tggagaccct ctgtgcaccc
     3361 caggtctgtc ccctgcctct taactccacc acggaagctg tgcacatgct tccgcacgca
     3421 ggggcaccgc ccttggatac tgtgtatcca gcacccgaga agaggccttc agccaggacc
     3481 caggggcccg tgaagtgtga gagcccagca acgactctgt gggcgcaaag tgagctgcca
     3541 gattttggag gcaacgtcct tctggcccct gcagccccgg cgctttatgt gtgtgcaaaa
     3601 cctcagccac ctgttgttca gcctccagaa gaggcgatgt ccgggcagca gtcacggaac
     3661 ccaagctcgg cggccccggt gcagagccga ggtggcattg gtgcttctga gaaccttgag
     3721 aatcctccca aaatgggaga ggaggaggcc cttcagtccc aggcgagttc tggttatgca
     3781 agtttattat cctcaccgcc cactgagtct ctgcagaatc ctccagtctt gattgctcag
     3841 cctgatcaca gctataatct ggctcagccc attaactttt ctgtgtcctt atcgaactct
     3901 catgagaaga atcagtcctg gagagaggct ttggtggggg atagacctgc agtcagcagt
     3961 tgggctctcg gtggtgattc tggggagaac acttctttgt ctgggattcc aaccagctct
     4021 gtccttagct tgtctctgcc tagcagtgtt gcccaaagta attttccaca aggttctggt
     4081 gcttccgaaa tggtttctaa tcagcctgct aatttgctgg ttcaaccacc atcccagcca
     4141 gttccagaga acttggttcc agaaagtcaa aaggatcgta aggcaggaag tgctcttccc
     4201 ggatttgcta atagccctgc tggaagcaca agtgtggtgt tagttccacc tgcacacggc
     4261 accctggtgc ctgatggtaa taaggcaaac cattccagtc atcaggaaga cacttacgga
     4321 gccctagact ttaccttaag caggactttg gaaaatcctg taaacgtgta caacccgtcc
     4381 cattctgaca gcctcgcttc tcagcaaagt gttgccagtc atcccagaca atctgggcct
     4441 ggggcgccta accttgaccg tttttatcag caggtcacga aagatgccca gggccagcct
     4501 ggcctcgaaa gagcccagca ggagctggtg ccaccccagc aacaggcttc tcccccacaa
     4561 ctacccaaag ccatgttttc ggagctgtca aatccagaaa gtctgcccgc acagggacag
     4621 gcccagaact cagcacagtc accagcaagt ctggttctgg tcgacgcggg tcagcagctg
     4681 ccccctcggc ctcctcagtc ctctagcgtg tctctggtgt ccagtggctc cggccaggca
     4741 gctgtgccgt cagagcagcc gtggccacag ccagtgcctg cacttgcccc cggcccaccg
     4801 cctcaggacc tggccgccta ctactactac cggcctttgt acgatgccta ccagcctcag
     4861 tactctttgc cgtacccacc ggagcctggc gcagcctccc tctattacca ggatgtctac
     4921 agcctctatg agcctcgata caggccctat gatggtgctg cgtctgctta cgcccagaac
     4981 taccgctatc ccgagcccga gcggcccagc tcccgagcca gccactcctc ggaacggcca
     5041 cctcccaggc aaggatatcc tgaaggatac tatagttcca aaagtggatg gagcagtcag
     5101 agcgattact atgcaagcta ttactccagc cagtacgatt atggagatcc aggtcactgg
     5161 gatcgttacc actacagtgc tagagtcagg gacccccgca cctatgaccg gaggtattgg
     5221 tgtgatgcag agtatgacgc atacaggaga gagcactctg ccttcgggga caggcccgag
     5281 aaacgtgaca acaactggag gtacgatcct cgcttcacgg ggagttttga cgatgacccc
     5341 gatccgcaca gagaccctta tggggaagag gtggaccggc gcagcgtcca cagcgagcac
     5401 tcggcacgga gcctgcacag cgcacacagc ctggccagcc gccgcagcag cctcagctcc
     5461 cactcgcacc agagtcagat ttacagaagc cacaatgtgg ctgccggttc ctacgaggcc
     5521 ccgcttcctc caggctcctt tcacggcgat tttgcctacg gcacctaccg cagcaatttc
     5581 agcagtggcc ccggcttccc agagtatggc taccctgccg acaccgtctg gcctgccatg
     5641 gagcaagttt catcaagacc aacttctcct gaaaaatttt cagtgcctca tgtctgtgcc
     5701 aggtttggcc ctggcggtca gcttatcaaa gtgattccca atctgccttc agaaggacag
     5761 ccggccttgg tggaggtcca cagcatggag gccttgctgc agcacacgtc tgagcaggag
     5821 gagatgcggg cgttcccggg acccctggcc aaagacgaca cccataaggt ggatgtcatt
     5881 aattttgcac agaacaaagc tatgaaatgt ttgcagaatg aaaacttaat tgacaaagag
     5941 tctgcaagtc ttctttggaa ttttattgtt ctcttatgca gacaaaatgg gaccgtggta
     6001 gggaccaaca ttgcggagct tctgttacga gaccacagaa cagtgtggct tcctgggaag
     6061 tcgcccaatg aagcaaacct gattgatttc acgaatgagg cagtggagca ggtggaagag
     6121 gaggagtctg gtgaggccca gctctctttc ctcactggtg gtccggcggc tgccgccagc
     6181 tcgctcgaga gagagaccga gaggttcagg gagctgttgc tgtatggccg taagaaggat
     6241 gctttggagt ctgcaatgaa gaatggcctg tggggtcacg ctctgctact tgcaagtaag
     6301 atggacagcc ggacacacgc ccgagtcatg accaggtttg ctaacagcct cccaatcaac
     6361 gaccctctgc agacagtcta ccagctcatg tccggacgga tgcctgccgc gtccacgtgc
     6421 tgtggagacg agaaatgggg agattggagg ccgcacctcg ccatggtctt gtccaacttg
     6481 aacaacaaca tggacgtcga gtccaggacg atggctacca tgggcgacac tctggcttca
     6541 aggggcctct tggatgcggc ccacttctgc tacctcatgg cccaggcggg atttggtgtt
     6601 tacacgaaga aaactacaaa gcttgtctta atcggatcca atcacagttt gccattctta
     6661 aagttcgcaa ccaacgaagc aatccagagg acggaagcct atgagtacgc ccagtccctg
     6721 ggtgccgaga cctgccccct gcctagtttc caggtgttta agttcatcta ctcctgccgc
     6781 ctggcggaaa tggggctggc cacgcaagcc ttccactact gtgaggccat cgcgaagagc
     6841 atcctgacgc agccgcacct gtattccccg gtgttgatca gccagcttgt gcagatggct
     6901 tcccagttac gactcttcga tccccagctg aaagagaagc cagaagagga gtccttggcc
     6961 gcacccacgt ggctggttca cctgcagcag gtggagcggc agattaagga gggggctgga
     7021 gtatggcatc aggatggagc cctcccgcag cagtgtcctg gcactccgag ttccgagatg
     7081 gagcagttgg acaggccagg actcagtcag ccaggagccc tggggatcgc caaccctctg
     7141 ctggcggtgc ctgcaccgag ccctgagcac tcgagcccga gcgtgcggct gctgccctca
     7201 gctccgcaga cgctccctga cggcccattg gccagtcctg ccagagtgcc gatgttccca
     7261 gtgccactgc ccccggggcc cctggagccg ggtcctggct gtgtgacccc agggcctgca
     7321 cttggcttcc tggagccctc cgggcctggc ctcccacctg gtgtgccacc tctgcaggaa
     7381 aggagacact tgctccagga agccaggagc ccagacccag ggatagtgcc gcaggaggcg
     7441 cctgttggaa actcactttc cgagctaagc gaagaaaatt ttgatggaaa atttgctaat
     7501 ctgaccccct cgaggacggt gccagactcg gaggcccccc cagggtggga tcgtgccgac
     7561 tcgggtccca cgcagccacc tctgtctctc tcacccgctc ccgaaacaaa gagacccgga
     7621 caggcagcca agaaagaaac gaaggaacct aagaagggtg aatcctggtt ctttcgttgg
     7681 ctacctggaa agaaaaagac agaagcttat ttgccagatg acaagaacaa atcgattgtt
     7741 tgggatgaaa agaaaaacca gtgggtgaat ttaaatgagc cagaagagga gaagaaagcc
     7801 ccgcccccac ctccaacctc gatgcccaag actgtgcaag ctgccccgcc tgccctccca
     7861 gggcctcctg gagcccccgt gaacatgtac tctagaagag cagcaggaac cagagctcgc
     7921 tacgttgacg tcctgaaccc aagcgggacc cagcggagcg agccggctct cgctcctgcg
     7981 gactttgtcg ctccactcgc gccactccca attccttcta acttgttcgt gccaacccca
     8041 gatgcagaag aaccacagct tccagacggg actggcaggg aagggcctgc agcagctagg
     8101 ggcctggcca atccagagcc tgccccagag cccaaggttt taagctctgc agcgtcactc
     8161 cctggctctg aactcccctc ctccaggcct gagggttccc agggaggaga gctttcgcgc
     8221 tgtagttcaa tgagttcatt atcacgtgaa gtgagccagc attttaatca ggctcctggc
     8281 gacctccctg ctgcaggggg ccctcccagc ggggccatgc ccttctacaa ccctgctcag
     8341 ctggcacagg cctgcgccac ctccgggagc tcaaggctag ggaggattgg ccagaggaag
     8401 cacctggtgc tgaactgaga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
     8461 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     8521 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     8581 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8641 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8701 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     8761 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     8821 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     8881 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     8941 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     9001 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     9061 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     9121 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     9181 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9241 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     9301 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9361 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9421 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9481 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9541 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9601 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     9661 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     9721 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     9781 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     9841 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     9901 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     9961 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
    10021 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
    10081 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
    10141 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    10201 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
    10261 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
    10321 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
    10381 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
    10441 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
    10501 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
    10561 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    10621 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
    10681 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
    10741 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
    10801 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
    10861 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
    10921 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10981 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    11041 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    11101 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    11161 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    11221 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11281 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11341 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11401 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    11461 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    11521 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    11581 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    11641 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    11701 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    11761 ttctgtggat aaccgtatta ccgccatgca t
//
