LOCUS       sequence-25416-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K
  TITLE     Induced pluripotent stem cells generated without viral integration.
  JOURNAL   Science. 2008 Nov 7;322(5903):945-9. doi: 10.1126/science.1162494. 
            Epub 2008 Sep 25.
  PUBMED    18818365
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1162494"; journalName: "Science"; date: "2008-11-7-
            7"; volume: "322"; issue: "5903"; pages: "945-9"
COMMENT     Sequence Label: pAd-cMyc T58A
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          139..614
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      693..702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             709..735
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             715..741
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             754..780
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggtnnnnagg ntnnnaagcn gagntcgttn ngtgaaccgt cagatcgcct ggagacgcca
       61 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc cctcgaagct
      121 gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc cccttctttt
      181 ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacacat attgaccaaa
      241 tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat atactttttt
      301 gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata atgatacaat
      361 gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg gttaaggcaa
      421 tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg taagaggttt
      481 catattgcta atagcagcta caatccagct accattctgc ttttatttta tggttgggat
      541 aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta
      601 tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat cactttggca
      661 aagaattgat cttcggccag cgactagtag aagccaccat gggatagcta tccttatgac
      721 gtgcctgact atgccagcct gatgctgatg ngctatcctt atgacgtgcc tgactatgcc
      781 cagcnngatg ctgatgagct atccctt
//
