LOCUS       sequence-254162-       11289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses uORF1 -36xMashTag with MoonTag as readout for 
            reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins 
            in 3' UTR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11289)
  AUTHORS   Boersma S, Khuperkar D, Verhagen BMP, Sonneveld S, Grimm JB, Lavis 
            LD, Tanenbaum ME
  TITLE     Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity
            in mRNA Decoding.
  JOURNAL   Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi: 
            10.1016/j.cell.2019.05.001.
  PUBMED    31178119
REFERENCE   2  (bases 1 to 11289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 5. pii: S0092-8674(19)30499-4. doi: 10.1016/j.cell.2019.05.001."
COMMENT     Sequence Label: uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7
            (u...
FEATURES             Location/Qualifiers
     source          1..11289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1019..1028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1047..1056
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1129..1138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1170..1226
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1228..1272
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1275..1331
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1333..1377
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1380..1436
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1438..1482
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1485..1541
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1543..1587
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1590..1646
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1648..1692
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1695..1751
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1753..1797
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1800..1856
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1858..1902
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1905..1961
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1963..2007
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2010..2066
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2068..2112
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2115..2171
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2173..2217
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2220..2276
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2278..2322
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2325..2381
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2383..2427
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2430..2486
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2488..2532
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2535..2591
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2593..2637
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2640..2696
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2698..2742
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2745..2801
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2803..2847
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2850..2906
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2908..2952
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2955..3011
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3013..3057
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3060..3116
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3118..3162
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3165..3221
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3223..3267
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3270..3326
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3328..3372
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3375..3431
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3433..3477
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3480..3536
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3538..3582
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3585..3641
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3643..3687
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3726..3782
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3784..3828
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3831..3887
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3889..3933
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3936..3992
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3994..4038
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4041..4097
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4099..4143
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4146..4202
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4204..4248
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4251..4307
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4309..4353
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4356..4412
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4414..4458
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4461..4517
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4519..4563
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4566..4622
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4624..4668
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4729..4773
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4834..4878
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4881..4937
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4939..4983
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     complement(7297..7314)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7303..7527
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7573..8001
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7660..7679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7870..7891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8010..8030)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8015..8344
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8195..8330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8257..8276
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8392..8439
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8458..8832
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8962..9083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8999..9018)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9053..9072
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9132..9148)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9132..9148)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9145..9167)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9156..9172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9180..9186,9187..9204,9205..9210))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9225..9246
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9363..9380)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9534..10122)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9614..9633)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10293..11084,11085..11153))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10916..10935
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11154..11258)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgtc
      961 cggacctcag cttcaggcgg ctgcaacggc tactcacagg ccagtcagta catcgattgc
     1021 caccatggac ctctcaactt ctcgtcgcca ccatggggac cctctgctgc gctaagatat
     1081 catctgctca ttcaccaact tcctcctccg tcagccgttc gaaagcttgc caccatgggc
     1141 gccatcatac acgcggccgc accggtcaag aagaacttct cagcaagaat tatcatttgg
     1201 agaacgaagt ggctcgtctc aagaaacaag aacgagcaag aattactgga attggacaag
     1261 tgggcaagcc tccagaagaa ctgctttcaa agaattacca cctggaaaac gaggttgcca
     1321 gactcaaaaa gcaagaacga gcaggaactc ctcgaactcg acaagtgggc ttctctccag
     1381 aggagttact cagcaaaaat tatcatttgg agaacgaagt tgcacgactc aagaaagaaa
     1441 aacgaacagg agttactcga gctcgacaag tgggccagcc tccagaggag ttactctcga
     1501 aaaattatca tctcgaaaac gaagtggctc ggctcaaaaa gcaagaacga acaagaactc
     1561 ctcgaactgg acaagtgggc atctctcgag aagagctatt atccaaaaac taccacctcg
     1621 aaaacgaggt ggcacgcctc aaaaagcaag aacgagcaag aattgttgga actcgacaag
     1681 tgggcaagcc ttcagaagag ctgctatcca agaattatca tctcgagaac gaggtggcgc
     1741 gtctcaagaa acaagaacga gcaagaatta ctcgaactcg acaagtgggc ttctctcgag
     1801 aggaactgct ctcgaagaac tatcatctgg aaaacgaggt cgctcgactc aaaaagcaaa
     1861 aacgaacagg agctgctgga gttggacaag tgggccagcc tccagaggaa ctactttcaa
     1921 agaattacca cctcgaaaac gaagttgctc gactcaaaaa gcaagaacga acaggaatta
     1981 ctcgaactcg acaagtgggc atctctccag aagaattact cagcaaaaat tatcatctgg
     2041 aaaacgaggt ggcgagactc aaaaagcaag aacgagcagg aactcctcga attggacaag
     2101 tgggcaagcc tgcagaagag ttgctatcga aaaattatca tctggagaac gaagttgcaa
     2161 ggctcaaaaa ggaaaaacga gcaagaacta ctcgaactcg acaagtgggc ttctctgcag
     2221 aggagttgct ctcgaaaaac taccacttgg aaaacgaggt cgcgaggctc aaaaagcaaa
     2281 aacgaacaag agctgctcga gctggacaag tgggccagcc tggagaggag ttactcagca
     2341 aaaactatca tctcgagaac gaagtcgcgc gcctcaagaa agaagaacga acaagaacta
     2401 ctcgaactgg acaagtgggc atctctgcag aagaactctt atcgaagaac taccacctcg
     2461 aaaacgaggt cgccaggctc aaaaaggaag aacgagcagg aactgctgga gctggacaag
     2521 tgggcctctc ttcagaggaa ctcctcagca agaactacca cttggagaac gaggtcgcga
     2581 gactcaagaa agaaaaacga acaagagcta ctcgagctcg acaagtgggc ctcccttcag
     2641 aggaattact cagcaagaat tatcatttgg agaacgaagt cgccaggctc aagaaacaaa
     2701 aacgaacagg aattactcga gctggacaag tgggcaagcc tggagaggaa ttactcagca
     2761 aaaactacca cttggaaaac gaagtcgcca ggctcaaaaa agaaaaacga acaggagtta
     2821 ctggagctcg acaagtgggc ttctctgcag aagagttact cagcaaaaat taccacttgg
     2881 agaacgaagt ggcaaggctc aagaaagaag aacgagcagg aactgctgga gctcgacaag
     2941 tgggcctctt tgcggaggag ctcttatcga agaactacca cctcgagaac gaagtcgccc
     3001 gcctcaagaa acaaaaacga acaggagcta ctcgagttgg acaagtgggc ctccctggag
     3061 aagagctcct cagcaagaac taccacttgg aaaacgaggt ggcacgcctc aagaaacaaa
     3121 aacgaacaag aactcctcga gttggacaag tgggcaagcc tcgagaagag ttactatcca
     3181 agaattatca tctcgagaac gaggtggctc gactcaaaaa gcaaaaacga acaggagctc
     3241 ctggagctcg acaagtgggc ttctctccag aggaactcct gtcgaagaac tatcatctcg
     3301 aaaacgaggt tgcaagactc aaaaaggaag aacgagcagg aactcctgga gctcgacaag
     3361 tgggcctctc tggagaggaa ctactctcaa agaattacca cctggaaaac gaagttgcac
     3421 gtctcaagaa acaaaaacga acaggagtta ctggagctcg acaagtgggc ctccctcgag
     3481 aagaactgct ctcaaaaaat tatcatttgg aaaacgaggt tgcacgtctc aaaaaggaaa
     3541 aacgaacaag aattactcga gctcgacaag tgggcaagcc ttcagaggaa cttctgtcga
     3601 aaaattatca tctcgagaac gaagttgccc gactcaaaaa gaaaaaacga acaagagtta
     3661 ctggagctgg acaagtgggc ttctctgggg ttcgaagcag gggcacgcgg tccggggttc
     3721 gaagggagga acttctcagc aagaattatc atttggagaa cgaagtggct cgtctcaaga
     3781 aacaaaaacg aacaggaact cctggaattg gacaagtggg caagccttca gaagaactgc
     3841 tttcaaagaa ttaccatctg gaaaacgagg ttgccagact caagaagcaa gaacgagcag
     3901 gaattactcg aattggacaa gtgggcctcc ctccagagga gttgctcagc aagaattatc
     3961 atttggagaa cgaagttgca cgactcaaga aagaagaacg aacaggagtt actcgagctc
     4021 gacaagtggg ccagcctcca gaggaattac tctcgaaaaa ttatcatctc gaaaacgaag
     4081 tggcccggct caaaaagcaa gaacgaacag gaactcctcg agctcgacaa gtgggcctcc
     4141 ctcgagagga actattatcc aagaactacc acctcgaaaa cgaggtggca cgcctcaaga
     4201 agcaaaaacg aacaggaatt gttggaactc gacaagtggg caagccttca gaggaactac
     4261 tatccaaaaa ttatcatctc gagaacgagg tggcgcgtct caaaaagcaa aaacgagcag
     4321 gaactactgg aactcgacaa gtgggcctcc ctcgagagga attgctctcg aagaactatc
     4381 atctggagaa cgaggtcgct cgactcaaga agcaagaacg aacaggagct gctggaactg
     4441 gacaagtggg ccagcctcca gaagaacttc tttcaaaaaa ttaccacctc gaaaacgaag
     4501 ttgcgcgact caaaaagcaa aaacgagcag gagctcctcg aactcgacaa gtgggcatct
     4561 ctccagagga gttactcagc aagaattatc atctggaaaa cgaggtggcg agactcaaaa
     4621 agcaagaacg agcaagagct cctcgagctg gacaagtggg catccctgca gaagagttac
     4681 tcaggaaaaa ttatcatctg gaaaacgaag ttgcaaggct caaaaaggaa gaacgaacag
     4741 gagctactcg aactcgacaa gtgggcatcc ctgcagagga gctgctcagg aaaaactacc
     4801 acttggaaaa cgaggtcgcg aggctcaaga agcaagaacg agcaggaact gctcgagctg
     4861 gacaagtggg ccagcctgga gaagagcttc tcagcaagaa ctatcatctc gagaacgaag
     4921 tcgcgcgcct caagaaggaa aaacgagcaa gaactactcg agctcgacaa gtgggcaagc
     4981 ctgcagggcg tacggggacc ggtggagcga gctcattcgg gagaacatgc acatcaagct
     5041 atacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa
     5101 gccctacgag ggcacccaga ccatcagaat caaggtggtc gagggcggcc ctctcccctt
     5161 cgccttcgac atcctggcta cgagcttcct ctacggcagc aagaccttca tcaaccacac
     5221 ccagggcatc cccgacttct tcaagcagtc cttccctcag ggcttcacat gggagagagt
     5281 caccacatac gaagacgggg gcgtgctcac cgctacccag gacaccagcc tccaggacgg
     5341 ctgcctcatc tacaacgtca agatcagagg ggtcaacttc acatccaacg gccctgtcat
     5401 gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg ccgacggcgg
     5461 cctggaaggc agaaacgaca tggccctcaa gctcgtgggc gggagccatc tcatcgcaaa
     5521 catcaagacc acatacagat ccaagaaacc cgcaaagaac ctcaagatgc ctggcgtcta
     5581 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca
     5641 gcacgaggtg gcagtggcca gatactgcga cctcccaagc aaactggggc acaaactcaa
     5701 tccgcggggg tgagtgagtg aggggctagc ccggttgtac aggtaccgga tcctaaggta
     5761 cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc gactctagaa
     5821 accagcagag catatgggct cgctggctgc agtattcccg ggttcattag atcctaaggt
     5881 acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt cgactctaga
     5941 aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta gatcctaagg
     6001 tacctaattg cctagaaagg agcagacgat atggcgtcgc tccctgcagg tcgactctag
     6061 aaaccagcag agcatatggg ctcgctggct gcagtattcc cgggttcatt agatcctaag
     6121 gtacctaatt gcctagaaag gagcagacga tatggcgtcg ctccctgcag gtcgactcta
     6181 gaaaccagca gagcatatgg gctcgctggc tgcagtattc ccgggttcat tagatcctaa
     6241 ggtacctaat tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct
     6301 agaaaccagc agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta
     6361 aggtacctaa ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc
     6421 tagaaaccag cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatcct
     6481 aaggtaccta attgcctaga aaggagcaga cgatatggcg tcgctccctg caggtcgact
     6541 ctagaaacca gcagagcata tgggctcgct ggctgcagta ttcccgggtt cattagatcc
     6601 taaggtacct aattgcctag aaaggagcag acgatatggc gtcgctccct gcaggtcgac
     6661 tctagaaacc agcagagcat atgggctcgc tggctgcagt attcccgggt tcattagatc
     6721 ctaaggtacc taattgccta gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga
     6781 ctctagaaac cagcagagca tatgggctcg ctggctgcag tattcccggg ttcattagat
     6841 cctaaggtac ctaattgcct agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg
     6901 actctagaaa ccagcagagc atatgggctc gctggctgca gtattcccgg gttcattaga
     6961 tcctaaggta cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc
     7021 gactctagaa accagcagag catatgggct cgctggctgc agtattcccg ggttcattag
     7081 atcctaaggt acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt
     7141 cgactctaga aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta
     7201 gatctcgcga agggcgaatt cacgaggcgc gcccggatcg cgatcgcaat cttaattaac
     7261 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     7321 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7381 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7441 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7501 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     7561 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7621 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7681 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7741 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7801 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7861 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7921 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7981 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8041 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8101 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8161 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8221 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8281 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8341 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     8401 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     8461 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     8521 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     8581 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     8641 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     8701 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     8761 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     8821 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8881 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8941 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     9001 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9061 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9121 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9181 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9241 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9301 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9361 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9421 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9481 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9541 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9601 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9661 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9721 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9781 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9841 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9901 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9961 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10021 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10081 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10141 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10201 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10261 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10321 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10381 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10441 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10501 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10561 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10621 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10681 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10741 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10801 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10861 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10921 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10981 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11041 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11101 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11161 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11221 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11281 cctgacgtc
//
