LOCUS       sequence-254189-        3018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 2 module- Cyc1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G
  TITLE     COSPLAY: An expandable toolbox for combinatorial and swift 
            generation of expression plasmids in yeast.
  JOURNAL   PLoS One. 2019 Aug 28;14(8):e0220694. doi: 
            10.1371/journal.pone.0220694. eCollection 2019.
  PUBMED    31461461
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-28"; volume: "14(8):e0220694. doi"; pages: " 
            10.1371/journal.pone.0220694. eCollection 2019"
COMMENT     Sequence Label: pMV20
FEATURES             Location/Qualifiers
     source          1..3018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     85..107
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     99..116
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     100..116
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(518..534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(518..534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(531..553)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    542..558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(566..572,573..590,591..596))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    611..632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(749..766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(920..1508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1000..1019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1679..2470,2471..2539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2302..2321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2540..2644)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2712..2730
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2768..2790)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2890..2909
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
       61 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
      121 tcgagctcgg tacctcgcga atgcatctag tcgatcccgg tctcgtatgc atttggcgag
      181 cgttggttgg tggatcaagc ccacgcgtag gcaatcctcg agcagatccg ccaggcgtgt
      241 atatatagcg tggatggcca ggcaacttta gtgctgacac atacaggcat atatatatgt
      301 gtgcgacgac acatgatcat atggcatgca tgtgctctgt atgtatataa aactcttgtt
      361 ttcttctttt ctctaaatat tctttcctta tacattagga cctttgcagc ataaattact
      421 atacttctat agacacgcaa acacaaatac acacactaag aacagagacc gggcccgtcg
      481 actgcagagg cctgcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      541 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      601 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      661 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      721 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      781 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      841 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      901 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      961 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1021 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1081 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1141 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1201 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1261 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1321 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1381 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1441 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1501 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1561 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1621 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1681 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1741 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1801 gtgctgcaat gataccgcga gatccacgct caccggctcc agatttatca gcaataaacc
     1861 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1921 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1981 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2041 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2101 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2161 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2221 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2281 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2341 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2401 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2461 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2521 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2581 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2641 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     2701 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     2761 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     2821 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     2881 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     2941 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     3001 actgttggga agggcgat
//
