LOCUS       sequence-254211-       10906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To make virus expressing Doc2alpha D291 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10906)
  AUTHORS   Courtney NA, Briguglio JS, Bradberry MM, Greer C, Chapman ER
  TITLE     Excitatory and Inhibitory Neurons Utilize Different Ca(2+) Sensors 
            and Sources to Regulate Spontaneous Release.
  JOURNAL   Neuron. 2018 Jun 6;98(5):977-991.e5. doi: 
            10.1016/j.neuron.2018.04.022. Epub 2018 May 10.
  PUBMED    29754754
REFERENCE   2  (bases 1 to 10906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.04.022"; journalName: "Neuron"; date: 
            "2018-06-6- 6"; volume: "98"; issue: "5"; pages: "977-991.e5"
COMMENT     Sequence Label: pFSW Doc2alpha D291N mutant
FEATURES             Location/Qualifiers
     source          1..10906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2365..2811
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    4045..4616
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4066..4618
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4210..4227)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4437..4456
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4618..4620,4621..4623,4624..5337)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4618..4620,4621..4623,4624..5334)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4663..4684)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4924..4943)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5271..5292
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5361..5949
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5414..5434)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5832..5843)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5952..5968)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5953..5969)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6474..6654
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6680..6697)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6686..6910
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6956..7384
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7043..7062)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7253..7274
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7393..7413)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7398..7727
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7578..7713
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7640..7659
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7775..7822
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7841..8215
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8345..8466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8382..8401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8436..8455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8515..8531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8515..8531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8528..8550)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8539..8555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8563..8569,8570..8587,8588..8593))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8608..8629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8746..8763)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8917..9505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8997..9016)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9676..10467,10468..10536))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10299..10318
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10537..10641)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10716..10735)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaagtgca agtggtttta ggaccaggat gaggcggggt
     2401 gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc
     2461 caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc
     2521 gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac
     2581 cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgta aactcccctt
     2641 cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg
     2701 cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg
     2761 cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca ggtacggcta
     2821 ggccaccatg agaggacgaa gaggtgatcg aatgaccatc aacatccagg agcacatggc
     2881 aatcaacgtg tgccctggac ccatcaggcc catccgccag atctccgact acttccctcg
     2941 cagaggacca ggaccagagg gtggcggcgg aggaaggggc ttcggggaag ctccagctca
     3001 tctggcccct ctggctctgg ctccacctgc agctctcctt ggagccacta cacccgacga
     3061 tggagctgag gtagacagct acgactcgga tgataccact gccctgggca cgctggaatt
     3121 tgaccttctc tatgatcagg cttcctgcat gctgcactgt agaatcctca gggccaaggg
     3181 cctcaagccc atggatttca acggcctggc tgacccctat gtaaagctgc acctcctgcc
     3241 aggagcctgc aaggccaata agctaaaaac caagacacag aggaacacac tgaacccagt
     3301 gtggaacgag gagctgacgt acagtggaat cacagatgac gacatcaccc acaaggtgct
     3361 caggatctcg gtctgtgatg aggacaagct gagtcacaat gaatttattg gggagatccg
     3421 agtgcccctc cgccgcctca agccttcaca gaagaagcat tttaacatct gccttgagcg
     3481 tcaggtcccg cttccttcgc cctcttcaat gtctgcggcg ctgaggggca tatcctgtta
     3541 cctgaaggag ctagagcagg cagagcaggg acctgggctg ttggaagagc gcgggcgcat
     3601 cctgctgagc ctcagctaca gctctcggcg gcatgggctg ctggtgggca ttgttcgctg
     3661 tgctcacctg gctgcaatgg atgttaacgg ctactctaac ccttatgtga agacgtactt
     3721 gagaccagac gtggataaga aatccaagca taagacgtgt gtaaagaaga agacgctgaa
     3781 cccggaattt aatgaggaat tcttttatga gatggaactc tccactctgg ctaccaagac
     3841 cctggaagtc acagtctggg actacgacat tggcaaatcc aatgacttca taggtggtgt
     3901 gtctctgggg ccaggagccc ggggagaggc ccagaaacac tggagagact gtctacatca
     3961 gccggacaca gcagtggagc gctggcatac cctgaccagc gagctgcccc cagcagcagg
     4021 ggcgttgccg ttggcctaga attccccctc tccctccccc ccctaacgtt actggccgaa
     4081 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     4141 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     4201 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     4261 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     4321 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     4381 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     4441 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     4501 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     4561 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     4621 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4681 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4741 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4801 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     4861 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4921 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4981 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5041 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     5101 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     5161 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     5221 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     5281 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaaat
     5341 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5401 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5461 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5521 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5581 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5641 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5701 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5761 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5821 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5881 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5941 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     6001 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     6061 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     6121 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6181 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     6241 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     6301 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     6361 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     6421 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     6481 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6541 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6601 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     6661 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6721 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6781 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6841 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6901 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6961 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7021 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7081 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7141 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7201 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7261 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7321 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7381 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7441 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7501 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7561 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7621 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7681 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7741 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     7801 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7861 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7921 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     7981 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     8041 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     8101 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8161 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     8221 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8281 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8341 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8401 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8461 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8521 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8581 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8641 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8701 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8761 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8821 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8881 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8941 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9001 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9061 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9121 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9181 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9241 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9301 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9361 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9421 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9481 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9541 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9601 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9661 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9721 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9781 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9841 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9901 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9961 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10021 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10081 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10141 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10201 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10261 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10321 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10381 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10441 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10501 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10561 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10621 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    10681 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    10741 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    10801 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    10861 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
