LOCUS       sequence-254215-       10933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To make virus expressing Doc2beta 6X mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Courtney NA, Briguglio JS, Bradberry MM, Greer C, Chapman ER
  TITLE     Excitatory and Inhibitory Neurons Utilize Different Ca(2+) Sensors 
            and Sources to Regulate Spontaneous Release.
  JOURNAL   Neuron. 2018 Jun 6;98(5):977-991.e5. doi: 
            10.1016/j.neuron.2018.04.022. Epub 2018 May 10.
  PUBMED    29754754
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.04.022"; journalName: "Neuron"; date: 
            "2018-06-6- 6"; volume: "98"; issue: "5"; pages: "977-991.e5"
COMMENT     Sequence Label: pFSW Doc2beta 6X mutant
FEATURES             Location/Qualifiers
     source          1..10933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             218..398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    445..570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1063..1296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1481..1525
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1823..1940
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1985..2431
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    3692..4263
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3713..4265
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3857..3874)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4084..4103
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4265..4267,4268..4270,4271..4984)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4265..4267,4268..4270,4271..4981)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4310..4331)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4571..4590)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4918..4939
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5008..5596
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5061..5081)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5479..5490)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5599..5615)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5600..5616)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6121..6301
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6327..6344)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6333..6557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6603..7031
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6690..6709)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6900..6921
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7040..7060)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7045..7374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7225..7360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7287..7306
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7422..7469
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7488..7862
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7992..8113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8029..8048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8083..8102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8162..8178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8162..8178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8175..8197)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8186..8202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8210..8216,8217..8234,8235..8240))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8255..8276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8393..8410)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8564..9152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8644..8663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9323..10114,10115..10183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9946..9965
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10184..10288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10363..10382)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10554..10933
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      901 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
      961 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1021 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1081 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1141 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1201 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1261 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1321 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1381 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1441 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1501 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1561 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1621 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1681 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1741 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     1801 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     1861 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1921 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa
     1981 ttaaagtgca agtggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac
     2041 cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc
     2101 agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc
     2161 gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg
     2221 cgcgctgacg tcactcgccg gtcccccgta aactcccctt cccggccacc ttggtcgcgt
     2281 ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg
     2341 gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag
     2401 cggaggagtc gtgtcgtgcc tgagagcgca ggtacggcta ggccaccatg accctcagga
     2461 ggcgggggga gaaggcaacc atttccattc aagagcatat ggctattgat gtctgccctg
     2521 ggccaatacg gcctattaag caaataagcg attacttccc acgatttcca cgaggactgc
     2581 cacctaccgc agccccaagg gcttccgccc ccccagacgc ccccgctcgg tcccctgcag
     2641 ccacagcagg cccacggtca ccttctgatg gggcccgaga tgacgatgaa gatgtcgacc
     2701 agcttttcgg agcttacgga gcctcccccg gtccctcacc aggtccttct cctgtgcgcc
     2761 cacctgccaa gccccccgaa gacgaacctg atgctgatgg ttacgagagc gacgattgta
     2821 ccgcattggg tactctggac ttcagcctcc tgtacgatca agagaataat gcacttcact
     2881 gcactatttc caaggctaaa ggcctcaagc ctatggatca taacggcttg gccgctccct
     2941 acgtcaagct ccatttgctt cccggggcta gtaaggctaa caagctccgg acaaaaacat
     3001 tgagaaatac actgaatcca tcctggaacg agacactcac atattatggt atcaccgatg
     3061 aagacatgat acgcaaaact ctgagaatct ctgtctgtgc cgaagctaaa tttaggcaca
     3121 atgaattcat tggcgagact cgagtacccc tcaaaaagtt gaaacccaat catactaaga
     3181 cctttagcat ctgtcttgaa aagcaactcc ctgtggataa ggctgaagat aaatcactcg
     3241 aggaaagggg gcgcatcctc atctccctca aatattcttc acaaaaacaa ggccttctgg
     3301 ttggaatcgt caggtgcgca catctggcag ctatggacgc taacggatat tctgctccct
     3361 atgttaagac atatctcaaa ccagacgttg acaaaaaaag taaacataaa actgctgtca
     3421 agaagaaaac attgaatcca gaatttaatg aggagttttg ctacgaaatc aaacatggtg
     3481 atcttgctaa gaagactctc gaggtaaccg tctgggccta tgccatcggg aaaagtaacg
     3541 attttatagg tggagtcgtt ctcggaataa atgccaaggg ggaaaggctg aagcattggt
     3601 tcgactgctt gaagaacaag gataagagaa tcgaacgatg gcacactctt acaaacgaga
     3661 tcccaggtgc cgttctcagt gattgaaatt ccccctctcc ctcccccccc taacgttact
     3721 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     3781 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     3841 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     3901 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     3961 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     4021 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     4081 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     4141 tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta
     4201 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     4261 taatatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4321 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     4381 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4441 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     4501 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4561 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4621 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4681 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     4741 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4801 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4861 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     4921 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     4981 gtaaaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     5041 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5101 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5161 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5221 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5281 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     5341 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5401 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5461 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5521 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5581 tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc
     5641 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     5701 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     5761 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5821 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     5881 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     5941 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     6001 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     6061 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     6121 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6181 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6241 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6301 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     6361 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6421 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6481 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6541 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     6601 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6661 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6721 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6781 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6841 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6901 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6961 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7021 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7081 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7141 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7201 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7261 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     7321 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     7381 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     7441 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     7501 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     7561 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     7621 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     7681 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     7741 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     7801 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     7861 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     7921 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     7981 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8041 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8101 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     8161 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8221 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8281 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8341 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8401 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8461 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8521 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8581 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8641 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8701 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8761 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8821 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8881 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8941 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9001 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9061 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9121 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9181 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9241 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9301 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9361 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9421 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9481 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9541 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9601 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9661 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9721 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9781 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9841 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9901 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9961 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10021 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10081 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10141 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10201 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10261 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10321 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    10381 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    10441 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
    10501 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
    10561 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
    10621 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
    10681 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
    10741 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
    10801 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
    10861 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
    10921 cgctattacc atg
//
