LOCUS       sequence-254240-       16223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, BBM, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16223)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 16223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM146
FEATURES             Location/Qualifiers
     source          1..16223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             952..2604
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1018..1036)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2612..2860
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        3350..3425
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     RBS             6535..6546
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    9944..9968
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11269..11898
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12327..13400
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13466..13660
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13896..13918)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14004..14144
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14059..14078
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14159..14176)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14330..14918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14410..14429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15005..15799)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtagagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
      121 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
      181 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
      241 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
      301 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
      361 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
      421 caggtatttc cgttggatac gaattattcg tacgaccctc ggtaccgatc ggcgcgcctg
      481 gcagacatac tgtcccacaa atgaagatgg aatctgtaaa agaaaacgcg tgaaataatg
      541 cgtctgacaa aggttaggtc ggctgccttt aatcaatacc aaagtggtcc ctaccacgat
      601 ggaaaaactg tgcagtcggt ttggcttttt ctgacgaaca aataagattc gtggccgaca
      661 ggtgggggtc caccatgtga aggcatcttc agactccaat aatggagcaa tgacgtaagg
      721 gcttacgaaa taagtaaggg tagtttggga aatgtccact cacccgtcag tctataaata
      781 cttagcccct ccctcattgt taagggagca aaatctcaga gagatagtcc tagagagaga
      841 aagagagcaa gtagcctaga agtagtcaag gcggcgaagt attcaggcac gtggccagga
      901 agaagaaaag ccaagacgac gaaaacaggt aagagctaag cttcctgcac catggaagac
      961 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga
     1021 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     1081 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg
     1141 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     1201 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     1261 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct
     1321 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc
     1381 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     1441 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca
     1501 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt
     1561 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga
     1621 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     1681 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     1741 ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa
     1801 agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa
     1861 tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc
     1921 ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact
     1981 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     2041 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     2101 gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac
     2161 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     2221 gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag
     2281 tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac
     2341 atcttcgacg ctggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     2401 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     2461 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     2521 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     2581 aagggcggaa agatcgccgt gtaactcgag atatgaagat gaagatgaaa tatttggtgt
     2641 gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga
     2701 atttgtggct ttttctaata ttaaatgaat gtaagatcac attataatga ataaacaaat
     2761 gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta
     2821 tgtgctatgg tatggactat ggaatatgat taaagataag gagctccggt gacggaccca
     2881 tggcttcgtt gaacaacgga aactcgactt gccttccgca caatacatca tttcttctta
     2941 gctttttttc ttcttcttcg ttcatacagt ttttttttgt ttatcagctt acattttctt
     3001 gaaccgtagc tttcgttttc ttctttttaa ctttccattc ggagtttttg tatcttgttt
     3061 catagtttgt cccaggatta gaatgattag gcatcgaacc ttcaagaatt tgattgaata
     3121 aaacatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg aatctgaaag
     3181 aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat aggcccattt
     3241 aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga agctgagttt
     3301 atatacagct agagtcgaag tagtgattgt tggtagtagc gactccatgg ttttagagct
     3361 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     3421 ggtgcttttt ttcccggggc gcgccgatat cgagctctcc cggcgcgcct gcagtgcagc
     3481 gtgacccggt cgtgcccctc tctagagata atgagcattg catgtctaag ttataaaaaa
     3541 ttaccacata ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata
     3601 tatttaaact ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt
     3661 agagaatcat ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa
     3721 caggactcta cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag
     3781 cttcacctat ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa
     3841 tggtttttat agactaattt ttttagtaca tctattttat tctattttag cctctaaatt
     3901 aagaaaacta aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata
     3961 aaataaagtg actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac
     4021 atttttcttg tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg
     4081 acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc
     4141 tctgtcgctg cctctggacc cctctcgaga gttccgctcc accgttggac ttgctccgct
     4201 gtcggcatcc agaaattgcg tggcggagcg gcagacgtga gccggcacgg caggcggcct
     4261 cctcctcctc tcacggcacc ggcagctacg ggggattcct ttcccaccgc tccttcgctt
     4321 tcccttcctc gcccgccgta ataaatagac accccctcca caccctcttt ccccaacctc
     4381 gtgttgttcg gagcgcacac acacacaacc agatctcccc caaatccacc cgtcggcacc
     4441 tccgcttcaa ggtacgccgc tcgtcctccc cccccccctc tctaccttct ctagatcggc
     4501 gttccggtcc atggttaggg cccggtagtt ctacttctgt tcatgtttgt gttagatccg
     4561 tgtttgtgtt agatccgtgc tgctagcgtt cgtacacgga tgcgacctgt acgtcagaca
     4621 cgttctgatt gctaacttgc cagtgtttct ctttggggaa tcctgggatg gctctagccg
     4681 ttccgcagac gggatcgatt tcatgatttt ttttgtttcg ttgcataggg tttggtttgc
     4741 ccttttcctt tatttcaata tatgccgtgc acttgtttgt cgggtcatct tttcatgctt
     4801 ttttttgtct tggttgtgat gatgtggtct ggttgggcgg tcgttctaga tcggagtaga
     4861 attaattctg tttcaaacta cctggtggat ttattaattt tggatctgta tgtgtgtgcc
     4921 atacatattc atagttacga attgaagatg atggatggaa atatcgatct aggataggta
     4981 tacatgttga tgcgggtttt actgatgcat atacagagat gctttttgtt cgcttggttg
     5041 tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta gatcggagta gaatactgtt
     5101 tcaaactacc tggtgtattt attaattttg gaactgtatg tgtgtgtcat acatcttcat
     5161 agttacgagt ttaagatgga tggaaatatc gatctaggat aggtatacat gttgatgtgg
     5221 gttttactga tgcatataca tgatggcata tgcagcatct attcatatgc tctaaccttg
     5281 agtacctatc tattataata aacaagtatg ttttataatt attttgatct tgatatactt
     5341 ggatgatggc atatgcagca gctatatgtg gattttttta gccctgcctt catacgctat
     5401 ttatttgctt ggtactgttt cttttgtcga tgctcaccct gttgtttggt gttacttcct
     5461 gcaggcttcc ctaacctttg cactgtccaa aatggcttcc tgatcccctc acttcctcga
     5521 atcaatctaa gaagaaactc aagccgcaac cattaggggc agattaattg ctgcactttc
     5581 agataatcaa gcatggccac tgtgaacaac tggctcgctt tctccctctc cccgcaggag
     5641 ctgccgccct cccagacgac ggactccaca ctcatctcgg ccgccaccgc cgaccatgtc
     5701 tccggcgatg tctgcttcaa catcccccaa gattggagca tgaggggatc agagctttcg
     5761 gcgctcgtcg cggagccgaa gctggaggac ttcctcggcg gcatctcctt ctccgagcag
     5821 catcacaagg ccaactgcaa catgataccc agcactagca gcacagtttg ctacgccagc
     5881 tcaggtgcta gcaccggcta ccatcaccag ctgtaccacc agcccaccag ctcagcgctc
     5941 cacttcgcgg actccgtaat ggtggcctcc tcggccggtg tccacgacgg cggtgccatg
     6001 ctcagcgcgg ccgccgctaa cggtgtcgct ggcgctgcca gtgccaacgg cggcggcatc
     6061 gggctgtcca tgattaagaa ctggctgcgg agccaaccgg cgcccatgca gccgagggtg
     6121 gcggcggctg agggcgcgca ggggctctct ttgtccatga acatggcggg gacgacccaa
     6181 ggcgctgctg gcatgccact tctcgctgga gagcgcgcac gggcgcccga gagtgtatcc
     6241 acgtcagcac agggtggagc cgtcgtcgtc acggcgccga aggaggatag cggtggcagc
     6301 ggtgttgccg gcgctctagt agccgtgagc acggacacgg gtggcagcgg cggcgcgtcg
     6361 gctgacaaca cggcaaggaa gacggtggac acgttcgggc agcgcacgtc gatttaccgt
     6421 ggcgtgacaa ggcatagatg gactgggaga tatgaggcac atctttggga taacagttgc
     6481 agaagggaag ggcaaactcg taagggtcgt caagtctatt taggtggcta tgataaagag
     6541 gagaaagctg ctagggctta tgatcttgct gctctgaagt actggggtgc cacaacaaca
     6601 acaaattttc cagtgagtaa ctacgaaaag gagctggagg acatgaagca catgacaagg
     6661 caggagtttg tagcgcctct gagaaggaag tccagtggtt tctccagagg tgcatccatt
     6721 tacaggggag tgactaggca tcaccaacat ggaagatggc aagcacggat tggacgagtt
     6781 gcagggaaca aggatcttta cttgggcacc ttcagcaccc aggaggaggc agcggaggcg
     6841 tacgacatcg cggcgatcaa gttccgcggc ctcaacgccg tcaccaactt cgacatgagc
     6901 cgctacgacg tgaagtccat cctggacagc agcgccctcc ccatcggcag cgccgccaag
     6961 cgcctcaagg aggccgaggc cgcagcgtcc gcgcagcacc accatgcggg tgtcgtttcc
     7021 tatgacgttg ggaggattgc cagccaactg ggagatggcg gtgccctcgc tgcggcctat
     7081 ggtgctcact atcacggtgc cgcgtggcca acgattgcat tccagccggg cgcggcgtcc
     7141 accggactgt accatcctta cgcgcagcag cctatgcgcg gcggtggatg gtgtaaacaa
     7201 gagcaagatc acgctgtgat agcagcggca cactccttgc aggatcttca tcatttgaat
     7261 ctcggagccg ccggggccca cgactttttc tcggcagggc agcaggccgc cgccgctgcg
     7321 atgcacggcc tgggtagcat cgacagtgcg tcgctggagc acagcaccgg ctccaactcc
     7381 gtcgtctaca acggcggggt cggcgacagc aacggcgcca gcgccgtcgg cggcagtggc
     7441 ggtggctaca tgatgccgat gagcgctgcc ggagcaacca ctacatcggc aatggtgagc
     7501 cacgagcagg tccatgcacg ggcctacgac gaagccaagc aggctgctca gatggggtac
     7561 gagagctacc tggtgaacgc ggagaacaat ggtggcggaa ggatgtctgc atgggggact
     7621 gtcgtgtctg cagccgcggc ggcagcagca agcagcaacg acaacatggc cgccgacgtg
     7681 ggccacggcg gcgcgcagct gttcagtgtc tggaacgaca cttaactcga gccctagact
     7741 tgtccatctt ctggattggc caagttaatt aatgtatgaa ataaaaggat gcacacatag
     7801 tgacatgcta atcactataa tgtgggcatc aaagttgtgt gttatgtgta attactaatt
     7861 atctgaataa gagaaagaga tcatccatat ttcttatcct aaatgaatgt cacgtgtctt
     7921 tataattctt tgatgaacca gatgcatttt attaaccaat tccatataca tataaatatt
     7981 aatcatatat aattaatatc aattgggtta gcaaaacaaa tctagtctag gtgtgttttg
     8041 ctaattattg ggggatagtg caaaaagaaa tctacgttct caataattca gatagaaaac
     8101 ttaataaagt gagataattt acatagattg cttttatcct ttgatatatg tgaaaccatg
     8161 catgatataa ggaaaataga tagagaaata attttttaca tcgttgaata tgtaaacaat
     8221 ttaattcaag aagctaggaa tataaatatt gaggagttta tgattagagc tcagtgtttg
     8281 atcgccggcg gtaccgagtg tacttcaagt cagtgggaaa tcaataaaat gattatttta
     8341 tgaatatatt tcattgtgca agtagataga aattacatat gttacataac acacgaaata
     8401 aacaaaaaaa gacaatccaa aaacaaacac cccaaaaaaa ataatcactt tagataaact
     8461 cgtatgagga gaggcacgtt cagtgactcg acgattcccg agcaaaaaaa gtctccccgt
     8521 cacacatgta gtgggtgacg caattatctt taaagtaatc cttctgttga cttgtcattg
     8581 ataacatcca gtcttcgtca ggattgcaaa gaattataga agggatccca ccttttattt
     8641 tcttcttttt tccatattta gggttgacag tgaaatcaga ctggcaacct attaattgct
     8701 tccacaatgg gacgaacttg aaggggatgt cgtcgatgat attataggtg gcgtgttcat
     8761 cgtagttggt gaaatcgatg gtaccgttcc aatagttgtg tcgtccgaga cttctagccc
     8821 aggtggtctt tccggtacga gttggtccgc agatgtagag gctggggtgt cggattccat
     8881 tccttccatt gtccttgtta aatcggccat ccattcaagg tcagattgag cttgttggta
     8941 tgagacagga tgtatgtaag tataagcgtc tatgcttaca tggtatagat gggtttccct
     9001 ccaggagtgt agatcttcgt ggcagcgaag atctgattct gtgaagggcg acacatacgg
     9061 ttcaggttgt ggagggaata atttgttggc tgaatattcc agccattgaa gctttgttgc
     9121 ccattcatga gggaattctt ccttgatcat gtcaagatat tcctccttag acgttgcagt
     9181 ctggataata gttctccatc gtgcgtcaga tttgcgagga gaaaccttat gatctcggaa
     9241 atctcctctg gttttaatat ctccgtcctt tgatatgtaa tcaaggactt gtttagagtt
     9301 tctagctggc tggatattag ggtgatttcc ttcaaaatcg aaaaaagaag gatccctaat
     9361 acaaggtttt ttatcaagct ggagaagagc atgatagtgg gtagtgccat cttgatgaag
     9421 ctcagaagca acaccaagga agaaaataag aaaaggtgtg agtttctccc agagaaactg
     9481 gaataaatca tctctttgag atgagcactt gggataggta aggaaaacat atttagattg
     9541 gagtctgaag ttcttactag cagaaggcat gttgttgtga ctccgagggg ttgcctcaaa
     9601 ctctatctta taaccggcgt ggaggcatgg aggcaggggt attttggtca ttttaataga
     9661 tagtggaaaa tgacgtggaa tttacttaaa gacgaagtct ttgcgacaag ggggggccca
     9721 cgccgaattt aatattaccg gcgtggcccc cccttatcgc gagtgcttta gcacgagcgg
     9781 tccagattta aagtagaaaa tttcccgccc actagggtta aaggtgttca cactataaaa
     9841 gcatatacga tgtgatggta tttgatggag cgtatattgt atcaggtatt tccgttggat
     9901 acgaattatt cgtacgaccc tcatagttta aactatcagt gtttgacagg atatattggc
     9961 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
    10021 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
    10081 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
    10141 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
    10201 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
    10261 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
    10321 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
    10381 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
    10441 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
    10501 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
    10561 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
    10621 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
    10681 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
    10741 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
    10801 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
    10861 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
    10921 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
    10981 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
    11041 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
    11101 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
    11161 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
    11221 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
    11281 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
    11341 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
    11401 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
    11461 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
    11521 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
    11581 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
    11641 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
    11701 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
    11761 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
    11821 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
    11881 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
    11941 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
    12001 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
    12061 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
    12121 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
    12181 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
    12241 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
    12301 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
    12361 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
    12421 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
    12481 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
    12541 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
    12601 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
    12661 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
    12721 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
    12781 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
    12841 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
    12901 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
    12961 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
    13021 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
    13081 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
    13141 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
    13201 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
    13261 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
    13321 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
    13381 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
    13441 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
    13501 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
    13561 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
    13621 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
    13681 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
    13741 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
    13801 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
    13861 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
    13921 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    13981 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
    14041 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    14101 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
    14161 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    14221 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    14281 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    14341 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    14401 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    14461 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    14521 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    14581 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    14641 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    14701 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    14761 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    14821 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    14881 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    14941 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
    15001 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
    15061 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    15121 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    15181 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    15241 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    15301 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    15361 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    15421 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    15481 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    15541 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    15601 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    15661 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    15721 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    15781 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    15841 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    15901 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    15961 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
    16021 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
    16081 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
    16141 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    16201 tcggggagct gttggctggc tgg
//
