LOCUS       sequence-254241-       14831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14831)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 14831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM136
FEATURES             Location/Qualifiers
     source          1..14831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             952..2604
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1018..1036)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2612..2860
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        3350..3425
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3951..4296
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4239..4259
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    6696..6870
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8552..8576
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9877..10506
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10935..12008
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12074..12268
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12504..12526)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12612..12752
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12667..12686
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12767..12784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12938..13526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13018..13037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13613..14407)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtagagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
      121 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
      181 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
      241 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
      301 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
      361 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
      421 caggtatttc cgttggatac gaattattcg tacgaccctc ggtaccgatc ggcgcgcctg
      481 gcagacatac tgtcccacaa atgaagatgg aatctgtaaa agaaaacgcg tgaaataatg
      541 cgtctgacaa aggttaggtc ggctgccttt aatcaatacc aaagtggtcc ctaccacgat
      601 ggaaaaactg tgcagtcggt ttggcttttt ctgacgaaca aataagattc gtggccgaca
      661 ggtgggggtc caccatgtga aggcatcttc agactccaat aatggagcaa tgacgtaagg
      721 gcttacgaaa taagtaaggg tagtttggga aatgtccact cacccgtcag tctataaata
      781 cttagcccct ccctcattgt taagggagca aaatctcaga gagatagtcc tagagagaga
      841 aagagagcaa gtagcctaga agtagtcaag gcggcgaagt attcaggcac gtggccagga
      901 agaagaaaag ccaagacgac gaaaacaggt aagagctaag cttcctgcac catggaagac
      961 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga
     1021 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     1081 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg
     1141 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     1201 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     1261 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct
     1321 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc
     1381 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     1441 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca
     1501 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt
     1561 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga
     1621 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     1681 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     1741 ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa
     1801 agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa
     1861 tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc
     1921 ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact
     1981 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     2041 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     2101 gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac
     2161 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     2221 gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag
     2281 tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac
     2341 atcttcgacg ctggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     2401 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     2461 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     2521 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     2581 aagggcggaa agatcgccgt gtaactcgag atatgaagat gaagatgaaa tatttggtgt
     2641 gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga
     2701 atttgtggct ttttctaata ttaaatgaat gtaagatcac attataatga ataaacaaat
     2761 gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta
     2821 tgtgctatgg tatggactat ggaatatgat taaagataag gagctccggt gacggaccca
     2881 tggcttcgtt gaacaacgga aactcgactt gccttccgca caatacatca tttcttctta
     2941 gctttttttc ttcttcttcg ttcatacagt ttttttttgt ttatcagctt acattttctt
     3001 gaaccgtagc tttcgttttc ttctttttaa ctttccattc ggagtttttg tatcttgttt
     3061 catagtttgt cccaggatta gaatgattag gcatcgaacc ttcaagaatt tgattgaata
     3121 aaacatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg aatctgaaag
     3181 aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat aggcccattt
     3241 aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga agctgagttt
     3301 atatacagct agagtcgaag tagtgattgt tggtagtagc gactccatgg ttttagagct
     3361 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     3421 ggtgcttttt ttcccggggc gcgccgatat cgagctctcc cgcagatttg ccttttcaat
     3481 ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg tatcatcaag
     3541 acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa ggttaaagat
     3601 gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag
     3661 atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc aaggcaagta
     3721 atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat ggagtcaaag
     3781 attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt catacagagt
     3841 ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacacactt
     3901 gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt
     3961 caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt
     4021 attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     4081 aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
     4141 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     4201 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     4261 tctatataag gaagttcatt tcatttggag agaacacggg ggactatgat ggcttcattg
     4321 tcttgtgttg aagacaagat gaaaacaagt tgtttggtta atggtggagg aactataaca
     4381 acaacaacat ctcaatctac cttgcttgaa gagatgaagc tgttgaaaga ccagtcaggt
     4441 acaagaaagc cggtaataaa ctcggagcta tggcacgctt gtgcaggccc tttggtgtgt
     4501 ctccctcaag ttgggagctt agtgtattac ttctcacaag gtcatagcga gcaggttgct
     4561 gtttcaacca gaagatcagc aacaacacaa gttcctaatt atccgaacct tccatctcag
     4621 ttgatgtgtc aagtccataa tgttactctt catgctgaca aagacagtga cgaaatctat
     4681 gctcagatga gtcttcaacc tgttcactct gagagagatg tgttccctgt accagacttt
     4741 ggaatgctga gaggaagtaa gcacccgact gagtttttct gcaaaacact tactgcaagt
     4801 gacacaagca cacatggagg tttctcagtg ccacgtagag ctgcagagaa gctatttcca
     4861 ccattggact actcagcaca gccgccaacg caagagcttg tagttcgaga tcttcatgag
     4921 aatacttgga catttcgcca tatctaccga gggcaaccaa agagacatct cctaactaca
     4981 ggatggagtt tgttcgttgg atcgaagaga ttgagagctg gggattctgt tttgttcatc
     5041 agggatgaga agtcacaact tatggtcggt gttaggcgtg ccaatcgcca acaaacagca
     5101 cttccttcat cagttctctc agcggatagt atgcacatcg gtgttcttgc tgctgctgct
     5161 cacgcaaccg ccaaccgtac tccttttttg atattctata atccaagagc ttgtccagca
     5221 gagttcgtga tccctctagc taagtaccgt aaggcgatat gcgggtctca gctctcagtt
     5281 ggtatgagat ttggaatgat gtttgaaact gaagattccg ggaaacgaag gtacatggga
     5341 actattgttg gaatcagcga tttggatccg ttgagatggc ctggttctaa gtggcgtaac
     5401 cttcaggtag aatgggatga gcctggatgt aatgataaac ctactcgggt cagtccatgg
     5461 gatatcgaaa cacctgaaag tctcttcatt tttccttctc tgacctcagg actcaaacgt
     5521 cagctccatc catcttactt tgctggtgaa actgaatggg gtagcttgat aaaacggcca
     5581 cttatacgtg ttcctgattc cgcgaatggg attatgccat atgcatcttt ccctagtatg
     5641 gcttcggagc agcttatgaa aatgatgatg aggcctcaca acaaccaaaa tgtaccatct
     5701 ttcatgtctg agatgcagca gaatattgta atggggaatg gaggtttgct aggagatatg
     5761 aagatgcagc aacccctgat gatgaaccag aaatctgaga tggtgcagcc acaaaacaag
     5821 ctaacagtga acccatctgc ttctaatacg agtggccaag aacagaatct ttcacagagt
     5881 atgagtgctc ctgctaaacc tgagaactct acactctctg gttgcagctc tggtagagtc
     5941 caacatggac ttgagcagtc aatggaacag gcaagccagg ttactacatc cacagtgtgt
     6001 aatgaggaaa aggttaatca gctacttcag aaaccgggtg cttcgtcgcc tgtacaagct
     6061 gatcaatgtc ttgacattac tcatcagatt taccaaccac agtctgatcc aataaatgga
     6121 ttctctttcc tggaaactga tgagctgaca tcacaagtct cttccttcca gtctcttgcc
     6181 ggatcataca agcaaccatt cattctatcc tcccaggatt cttcagctgt tgtgttaccg
     6241 gattccacaa actcaccgct gtttcatgat gtgtgggaca ctcagttgaa cggtctcaag
     6301 tttgaccagt tcagtccctt gatgcagcag gacctttatg ctagtcagaa tatctgtatg
     6361 agtaatagca caaccagtaa cattctagat cctccactct caaacacagt ccttgatgac
     6421 ttctgtgcca tcaaagacac tgatttccag aaccaccctt ctggttgttt ggttggaaac
     6481 aacaacacta gctttgctca agatgtccag tcgcagatca catcagctag ctttgcagac
     6541 tcacaggcct tctctcgcca agattttcca gataattctg gaggcactgg tacatcttca
     6601 agcaatgttg attttgatga ttgtagtctg cggcaaaata gtaaaggctc atcatggcag
     6661 aaaattgcga caccccgcgt ccgaacctac tcgagtttct ccataataat gtgtgagtag
     6721 ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga
     6781 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     6841 aaaccaaaat ccagtactaa aatccagatc ccccgaatta agtgtttgat cgccggcggt
     6901 accgagtgta cttcaagtca gtgggaaatc aataaaatga ttattttatg aatatatttc
     6961 attgtgcaag tagatagaaa ttacatatgt tacataacac acgaaataaa caaaaaaaga
     7021 caatccaaaa acaaacaccc caaaaaaaat aatcacttta gataaactcg tatgaggaga
     7081 ggcacgttca gtgactcgac gattcccgag caaaaaaagt ctccccgtca cacatgtagt
     7141 gggtgacgca attatcttta aagtaatcct tctgttgact tgtcattgat aacatccagt
     7201 cttcgtcagg attgcaaaga attatagaag ggatcccacc ttttattttc ttcttttttc
     7261 catatttagg gttgacagtg aaatcagact ggcaacctat taattgcttc cacaatggga
     7321 cgaacttgaa ggggatgtcg tcgatgatat tataggtggc gtgttcatcg tagttggtga
     7381 aatcgatggt accgttccaa tagttgtgtc gtccgagact tctagcccag gtggtctttc
     7441 cggtacgagt tggtccgcag atgtagaggc tggggtgtcg gattccattc cttccattgt
     7501 ccttgttaaa tcggccatcc attcaaggtc agattgagct tgttggtatg agacaggatg
     7561 tatgtaagta taagcgtcta tgcttacatg gtatagatgg gtttccctcc aggagtgtag
     7621 atcttcgtgg cagcgaagat ctgattctgt gaagggcgac acatacggtt caggttgtgg
     7681 agggaataat ttgttggctg aatattccag ccattgaagc tttgttgccc attcatgagg
     7741 gaattcttcc ttgatcatgt caagatattc ctccttagac gttgcagtct ggataatagt
     7801 tctccatcgt gcgtcagatt tgcgaggaga aaccttatga tctcggaaat ctcctctggt
     7861 tttaatatct ccgtcctttg atatgtaatc aaggacttgt ttagagtttc tagctggctg
     7921 gatattaggg tgatttcctt caaaatcgaa aaaagaagga tccctaatac aaggtttttt
     7981 atcaagctgg agaagagcat gatagtgggt agtgccatct tgatgaagct cagaagcaac
     8041 accaaggaag aaaataagaa aaggtgtgag tttctcccag agaaactgga ataaatcatc
     8101 tctttgagat gagcacttgg gataggtaag gaaaacatat ttagattgga gtctgaagtt
     8161 cttactagca gaaggcatgt tgttgtgact ccgaggggtt gcctcaaact ctatcttata
     8221 accggcgtgg aggcatggag gcaggggtat tttggtcatt ttaatagata gtggaaaatg
     8281 acgtggaatt tacttaaaga cgaagtcttt gcgacaaggg ggggcccacg ccgaatttaa
     8341 tattaccggc gtggcccccc cttatcgcga gtgctttagc acgagcggtc cagatttaaa
     8401 gtagaaaatt tcccgcccac tagggttaaa ggtgttcaca ctataaaagc atatacgatg
     8461 tgatggtatt tgatggagcg tatattgtat caggtatttc cgttggatac gaattattcg
     8521 tacgaccctc atagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     8581 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     8641 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
     8701 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     8761 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
     8821 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
     8881 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
     8941 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
     9001 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
     9061 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
     9121 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
     9181 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
     9241 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
     9301 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
     9361 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
     9421 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
     9481 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
     9541 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     9601 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     9661 cgagccgccc gcgcacggct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     9721 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
     9781 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     9841 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     9901 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     9961 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
    10021 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
    10081 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
    10141 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
    10201 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
    10261 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
    10321 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
    10381 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
    10441 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
    10501 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
    10561 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
    10621 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
    10681 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
    10741 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
    10801 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
    10861 tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag
    10921 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
    10981 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
    11041 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
    11101 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
    11161 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
    11221 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
    11281 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
    11341 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
    11401 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
    11461 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
    11521 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
    11581 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
    11641 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
    11701 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
    11761 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
    11821 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
    11881 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
    11941 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
    12001 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
    12061 aaggcctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
    12121 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
    12181 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
    12241 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
    12301 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
    12361 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
    12421 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
    12481 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggaaac ggtcacagct
    12541 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    12601 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
    12661 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    12721 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
    12781 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    12841 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    12901 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    12961 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    13021 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    13081 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    13141 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    13201 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    13261 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    13321 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    13381 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    13441 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    13501 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    13561 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca
    13621 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
    13681 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
    13741 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
    13801 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
    13861 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
    13921 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
    13981 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
    14041 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
    14101 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
    14161 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
    14221 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
    14281 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
    14341 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
    14401 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
    14461 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
    14521 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
    14581 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
    14641 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
    14701 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
    14761 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
    14821 tggctggctg g
//
