LOCUS       sequence-254242-       11814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, sgRNA targeting 
            PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 11814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM117
FEATURES             Location/Qualifiers
     source          1..11814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        966..1311
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1254..1274
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1329..2981
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1395..1413)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2991..3239
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        3732..3807
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5535..5559
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6860..7489
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7918..8991
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9057..9251
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9487..9509)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9595..9735
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9650..9669
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9750..9767)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9921..10509)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10001..10020)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10596..11390)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtagagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
      121 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
      181 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
      241 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
      301 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
      361 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
      421 caggtatttc cgttggatac gaattattcg tacgaccctc ggtaccgatc ggcgcgccag
      481 atttgccttt tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca
      541 gcaggtatca tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc
      601 aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa
      661 gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac
      721 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag
      781 gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa
      841 cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg
      901 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg
      961 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
     1021 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
     1081 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
     1141 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     1201 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     1261 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact
     1321 cctgcaggat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
     1381 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     1441 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
     1501 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     1561 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     1621 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     1681 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
     1741 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
     1801 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     1861 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
     1921 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
     1981 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     2041 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     2101 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
     2161 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
     2221 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
     2281 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
     2341 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     2401 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     2461 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
     2521 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     2581 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
     2641 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
     2701 tgctccaaca ccccaacatc ttcgacgctg gtgtcgcagg tcttcccgac gatgacgccg
     2761 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     2821 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     2881 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     2941 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgtg acgtcgacga tatgaagatg
     3001 aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc
     3061 ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg taagatcaca
     3121 ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg gatctcttct
     3181 gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt aaagataagc
     3241 cagagctctg gtgacggacc catggcttcg ttgaacaacg gaaactcgac ttgccttccg
     3301 cacaatacat catttcttct tagctttttt tcttcttctt cgttcataca gttttttttt
     3361 gtttatcagc ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat
     3421 tcggagtttt tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa
     3481 ccttcaagaa tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa
     3541 aaggcccctg ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag
     3601 tatttcttat ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag
     3661 ataagaaaac gaagctgagt ttatatacag ctagagtcga agtagtgatt gttggtagta
     3721 gcgactccat ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     3781 cttgaaaaag tggcaccgag tcggtgcttt ttttcccggg gcgcgccgat atcgagctct
     3841 cccggcgcgc cgatatcgag ctcagtgttt gatcgccggc ggtaccgagt gtacttcaag
     3901 tcagtgggaa atcaataaaa tgattatttt atgaatatat ttcattgtgc aagtagatag
     3961 aaattacata tgttacataa cacacgaaat aaacaaaaaa agacaatcca aaaacaaaca
     4021 ccccaaaaaa aataatcact ttagataaac tcgtatgagg agaggcacgt tcagtgactc
     4081 gacgattccc gagcaaaaaa agtctccccg tcacacatgt agtgggtgac gcaattatct
     4141 ttaaagtaat ccttctgttg acttgtcatt gataacatcc agtcttcgtc aggattgcaa
     4201 agaattatag aagggatccc accttttatt ttcttctttt ttccatattt agggttgaca
     4261 gtgaaatcag actggcaacc tattaattgc ttccacaatg ggacgaactt gaaggggatg
     4321 tcgtcgatga tattataggt ggcgtgttca tcgtagttgg tgaaatcgat ggtaccgttc
     4381 caatagttgt gtcgtccgag acttctagcc caggtggtct ttccggtacg agttggtccg
     4441 cagatgtaga ggctggggtg tcggattcca ttccttccat tgtccttgtt aaatcggcca
     4501 tccattcaag gtcagattga gcttgttggt atgagacagg atgtatgtaa gtataagcgt
     4561 ctatgcttac atggtataga tgggtttccc tccaggagtg tagatcttcg tggcagcgaa
     4621 gatctgattc tgtgaagggc gacacatacg gttcaggttg tggagggaat aatttgttgg
     4681 ctgaatattc cagccattga agctttgttg cccattcatg agggaattct tccttgatca
     4741 tgtcaagata ttcctcctta gacgttgcag tctggataat agttctccat cgtgcgtcag
     4801 atttgcgagg agaaacctta tgatctcgga aatctcctct ggttttaata tctccgtcct
     4861 ttgatatgta atcaaggact tgtttagagt ttctagctgg ctggatatta gggtgatttc
     4921 cttcaaaatc gaaaaaagaa ggatccctaa tacaaggttt tttatcaagc tggagaagag
     4981 catgatagtg ggtagtgcca tcttgatgaa gctcagaagc aacaccaagg aagaaaataa
     5041 gaaaaggtgt gagtttctcc cagagaaact ggaataaatc atctctttga gatgagcact
     5101 tgggataggt aaggaaaaca tatttagatt ggagtctgaa gttcttacta gcagaaggca
     5161 tgttgttgtg actccgaggg gttgcctcaa actctatctt ataaccggcg tggaggcatg
     5221 gaggcagggg tattttggtc attttaatag atagtggaaa atgacgtgga atttacttaa
     5281 agacgaagtc tttgcgacaa gggggggccc acgccgaatt taatattacc ggcgtggccc
     5341 ccccttatcg cgagtgcttt agcacgagcg gtccagattt aaagtagaaa atttcccgcc
     5401 cactagggtt aaaggtgttc acactataaa agcatatacg atgtgatggt atttgatgga
     5461 gcgtatattg tatcaggtat ttccgttgga tacgaattat tcgtacgacc ctcatagttt
     5521 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     5581 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     5641 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
     5701 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
     5761 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     5821 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     5881 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     5941 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     6001 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     6061 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     6121 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     6181 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
     6241 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
     6301 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
     6361 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
     6421 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
     6481 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
     6541 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
     6601 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
     6661 gctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
     6721 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
     6781 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
     6841 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
     6901 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
     6961 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
     7021 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
     7081 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
     7141 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
     7201 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
     7261 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
     7321 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
     7381 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
     7441 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
     7501 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
     7561 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
     7621 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
     7681 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
     7741 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
     7801 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
     7861 ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
     7921 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     7981 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     8041 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     8101 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     8161 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     8221 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     8281 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     8341 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     8401 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     8461 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     8521 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     8581 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     8641 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     8701 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     8761 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     8821 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     8881 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     8941 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     9001 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggcct ctttcctgtg
     9061 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     9121 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     9181 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     9241 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     9301 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     9361 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     9421 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
     9481 tgaaaacctc tgacacatgc agctcccgga aacggtcaca gcttgtctgt aagcggatgc
     9541 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     9601 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     9661 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     9721 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9781 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9841 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9901 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9961 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10021 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10081 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10141 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10201 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10261 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10321 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    10381 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10441 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10501 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10561 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
    10621 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
    10681 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
    10741 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
    10801 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
    10861 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
    10921 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
    10981 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
    11041 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
    11101 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
    11161 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
    11221 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
    11281 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
    11341 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
    11401 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    11461 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    11521 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
    11581 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
    11641 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
    11701 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
    11761 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctgg
//
