LOCUS       sequence-25426-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
  TITLE     A system for Cre-regulated RNA interference in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
  PUBMED    18779577
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13895-900."
COMMENT     Sequence Label: MSCV FLIP FF
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           65..186
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           213..227
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           255..381
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    411..444
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 acgagctgta cagtagcggc cgcaagcctt gttaagtgct cgcttcggca gcacatatac
       61 tatgttgaat gaggcttcag tactttacag aatcgttgcc tgcacatctt ggaaacactt
      121 gctgggatta cttcttcagg ttaacccaac agaaggctcg agaaggtata ttgctgttga
      181 cagtgagcga gctcccgtga attggaatcc tagtgaagcc acagatgtag gattccaatt
      241 cagcgggagc ctgcctactg cctcggaatt caaggggcta ctttaggagc aattatcttg
      301 tttactaaaa ctgaatacct tgctatctct ttgatacatt tttacaaagc tgaattaaaa
      361 tggtataaat taaatcactt ttttcaattg gaagactaat gcgtttaaac ataacttcgt
      421 ataatgtgta ctatacgaag ttatggcttc tagactccag agaaatgaag tccagggctt
      481 ggaggaggga gagggaaagc agcagcagca gcatccagga tgtgttctga accagcaggc
      541 ttatgccgcc acacttgacc agcttgtctc tatacacact gatactttta ttggagctca
      601 tgggattcgc gcccgagacg cgaagctcac aaaagtagtc gccctcatcc ttggt
//
