LOCUS       sequence-25427-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
  TITLE     A system for Cre-regulated RNA interference in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
  PUBMED    18779577
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13895-900."
COMMENT     Sequence Label: MSCV FLIP p53
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           61..182
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           210..224
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           252..378
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    408..441
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ctgtacagta gcggccgcaa gccttgttaa gtgctcgctt cggcagcaca tatactatga
       61 ttgaatgagg cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg
      121 ggattacttc ttcaggttaa cccaacagaa ggctcgagaa ggtatattgc tgttgacagt
      181 gagcgcccac tacaagtaca tgtgtaatag tgaagccaca gatgtattac acatgtactt
      241 gtagtggatg cctactgcct cggaattcaa ggggctactt taggagcaat tatcttgttt
      301 actaaaactg aataccttgc tatctctttg atacattttt acaaagctga attaaaatgg
      361 tataaattaa atcacttttt tcaattggaa gactaatgcg tttaaacata acttcgtata
      421 atgtgtacta tacgaagtta tggcttctag actccagaga aatgaagtcc agggcttgga
      481 ggagggagag ggaaagcagc agcagcagca tccaggatgt gttctgaacc agcaggctta
      541 tgccgccaca cttgaccagc ttgtctctat acacactgat acttttattg gagctcatgg
      601 gattcgcgcc cgagacgcga agctcacaaa agtagtcgcc ctcatccttg gtggtgaagt
      661 tggctagggt aaggaccttg atata
//
