LOCUS       sequence-254275-        5949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Toney LM, Cattoretti G, Graf JA, Merghoub T, Pandolfi PP, 
            Dalla-Favera R, Ye BH, Dent AL
  TITLE     BCL-6 regulates chemokine gene transcription in macrophages.
  JOURNAL   Nat Immunol. 2000 Sep;1(3):214-20.
  PUBMED    10973278
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Immunol."; date: "2000-09"; volume: "1(3)"; pages: "214-20"
COMMENT     Sequence Label:  pMCP-Luc (MCP-1 promoter in pGL3-basic)
FEATURES             Location/Qualifiers
     source          1..5949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1221..1230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1227..2879
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1293..1311)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2920..3041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2931..2950)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2985..3004
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3289..3306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3460..4048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3540..3559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4219..5010,5011..5079))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4842..4861
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5080..5184)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5211..5666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5298..5317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5508..5529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5797..5845
                     /note="synthetic polyadenylation signal"
     misc_feature    join(5859..5949,1)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5899..5918
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 tatcgatagg taccgagctc ttacgcgtgc tagcccgggt ccgctcagcc actggcacgc
       61 atgtgagagc gccactcttt ttatcaagag tctgctgaaa tgaattctaa gggctttcag
      121 attttatcgc ttcgatcaca ctgtttgtga agaaatctaa acctggaaag ctgaattaag
      181 ccagacattc cagttggctc actcatggtc cctggaaatc acgaaggaaa cagggcagag
      241 agctaccagg agtagtaaac gctaacacac aggcttctaa ttcatcatgg cttaaccaaa
      301 aggcatgttt ctccatggcc cgttagctgt ctgttagttt gaaggtaatg tgatgatagc
      361 caggctggag ctacctgggt cctgttccag ggactcttag ggcagttacc tacatgaccc
      421 ttctggacct cagctgcctg atctgagaat ggggagtaac agcatctact taccagagct
      481 ggggaaatta gagactgcac tgtgaagggt tccaaccact cctggcccag agtaagcact
      541 aggtgagttt tatatagaaa tttcttctgc accatgagct catgtatatg gctttccagg
      601 tctggacttt tacaactcag acagttcata tcaatgtcac aaagcttctt agtgctgcaa
      661 aatatctggt aatcaccaag tggagagaat gctgtattca acaaggcctg ataaccaagg
      721 actcagccga cttactgggg gtcctttccc agctgcaagg ttccttgagc caggggcaag
      781 ctaggctgtg tttccaagca tcttcttcct aaggacttta ggttccttgg ccacttcctt
      841 ttatttcagt gaaagcagag ccactccatt cacacttgtg gtcacagtag tacaattact
      901 gccaattctt ccctctttcc cccccccccc ctactccctg cgcagcttca tttgctccca
      961 ggagtggcta gaaaaatacc aaattccaac ccacagtttc tctcttccac ttcctggaaa
     1021 cacccgaggg ctctgcactt actcagcgga ttcaacttcc actttccatc acttatccag
     1081 ggtgatgcta ctccttggca ccaagcaccc tgcctgactc cacccccctg gcttacaata
     1141 aaaggctgcc tcagagcagc cagaagtgca ctcgagatct gcgatctaag taagcttggc
     1201 attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg
     1261 cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga
     1321 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca
     1381 cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
     1441 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
     1501 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
     1561 gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg
     1621 ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca
     1681 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
     1741 tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa
     1801 ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc
     1861 atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
     1921 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
     1981 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
     2041 tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat
     2101 tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
     2161 ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc
     2221 atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta
     2281 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
     2341 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg
     2401 tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
     2461 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
     2521 tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg
     2581 ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc
     2641 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
     2701 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
     2761 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
     2821 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat
     2881 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     2941 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     3001 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3061 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     3121 acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca
     3181 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     3241 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
     3301 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3361 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3421 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3481 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3541 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3601 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3661 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3721 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3781 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3841 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3901 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3961 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4021 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4081 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4141 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4201 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4261 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4321 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4381 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4441 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4501 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4561 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4621 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4681 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4741 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4801 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4861 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4921 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4981 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5041 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5101 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5161 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
     5221 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5281 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5341 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5401 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5461 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5521 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5581 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5641 taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga
     5701 agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag
     5761 taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta
     5821 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
     5881 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
     5941 acatttctc
//
