LOCUS       sequence-254276-        6951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Vasanwala FH, Kusam S, Toney LM, Dent AL
  TITLE     Repression of AP-1 function: a mechanism for the regulation of 
            Blimp-1 expression and B lymphocyte differentiation by the B cell 
            lymphoma-6 protooncogene.
  JOURNAL   J Immunol. 2002 Aug 15;169(4):1922-9.
  PUBMED    12165517
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2002-08-15"; volume: "169(4)"; pages: "1922-9"
COMMENT     Sequence Label: Blimp1pGL3 (Human Blimp1 promoter in pGL3-basic)
FEATURES             Location/Qualifiers
     source          1..6951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    587..635
                     /note="synthetic polyadenylation signal"
     misc_feature    649..740
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     689..708
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      2962..2971
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2968..4620
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3034..3052)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4661..4782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4672..4691)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4726..4745
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5030..5047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5201..5789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5281..5300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5960..6751,6752..6820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6583..6602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6821..6925)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt accgagctcg gatccactag taacggccgc
      781 cagtgtgctg gaattcgccc ttattttagg gttgcctggc taagccccac actggtattt
      841 gaagtttgcg tcctccctag actcaagccc atttcaccac ctctgaaggt gagtgcaggc
      901 tgatattaat taacagtgag ttgattcact ggcatcttcc agtcattaaa gcaaatattt
      961 ggcttaaaat agccttcttc ccacctcaaa tatcaaactt tttgcctatt cccttactct
     1021 atcatggttg actcaccagc agttgcatga tggtgtatgt ggcctaagaa cagagtttga
     1081 ttctaaaagg gaagtgaaat actctttaac atctgcaagt gagactgttt gaacagcagg
     1141 ccagccttca gtatgactac attgtttttc tgtccattgt ggtgctgact cagcatcagt
     1201 taataaaccc tcttgagcag gctggattcc tcttgtttaa ttatcagctt ggagctttct
     1261 gaggactctt aggaattgtt cattcagttt tccagcccca gcagatctgc cttctaaaag
     1321 tcctgcttcc tcttggtgct agcgtcccct ctggcataac atgcaatcac aggtgggcca
     1381 cgcagcaagc agcgagtgtg tgctaatcat gtcccctagt gggaatttaa ttgttctgta
     1441 ctgagagaga gattgcagca agactttaga caccatttcc tggcaaataa tcttttgcct
     1501 ctccccctct cccctaaaaa aaattctaga aaagttttct aaaagtgaga aaagaaattt
     1561 taaagataaa cagacatatt ttttaggatt gagttgggct tttgttttgc tttgtttcat
     1621 ttcttcctgt aaacaaatac tcagatgtcc atttcattgt atgactaagt tggtatcatt
     1681 aggttggaac tgggtgtgtg tatgtgggtg tgtgtgtggg tggatgtgtg tgcacacgtg
     1741 tatttaaaat gttagaaaag acattgcagg tcgaaggaga aatgatttta aaaaaaccta
     1801 cctatggtaa ggcaagcagg aaaaatggaa gacctggatt tcaatgatct aaataataat
     1861 tgaaatgggg tgtttgctat aaacattgtt tctctgaatt aaaggattcc ttgctgaagg
     1921 cacaagacca ttgaaggaag taaagcatct ggtttgtttt gtaatgagaa gcaggaatgc
     1981 aaggtccacg ctcttaataa taaacaaaca ggacattgta tgccatcatc acaggatgtc
     2041 cttccttcaa cagaggactg actgggctgg aggaaaagct ggccggggct cagaataagc
     2101 cccactaatt actgcctccg accgctttcc acttgcagtg atcagttcaa tattcctctg
     2161 aactgtgaaa cgactaatgc cctacatttt acttacactc ctgggaagtc agcctgtggg
     2221 ttctgggagg agaggaaagg aaaacaaaca atctgtagaa ttataacttt tgcctatttc
     2281 cctatttact aaatagttaa aagaggaaag ttagttctgc caagtttgaa cgttagcagg
     2341 agaaacggaa tgttacaact tttggggcgg ggggcgggga aacgtgcgtt acatacacaa
     2401 cagcttgagg accagacagc tccactgtat tacactagct gcaaaaacaa tttaacttgc
     2461 tcttttgaag taagatttgt gtcttttgta cctggggatt tgagctgaga aatcagaaac
     2521 tgtgtaggta aattttaagt ttccttaatt taaggaacgt gcgcccccta attctgccgc
     2581 gccaggaagg agggcgatct ggagtgttta gaatacaata gagcccaagt aagcgttgag
     2641 gttaagtgcc ttcaaaggga agtaagaaga ttccaagtca atgttgaaat acacatgcga
     2701 agagaggaag ctctcggcgg ctgtgctagc aatctggggg aaagccctgg gctcggccag
     2761 gtggtgttgg ccacgttggc cacgccccca cttcgcgcag ccgagtggct aaggaaatct
     2821 taagcaggga ggggaagcca gacggttaac acagacaaag tgctgccgtg acaagggcga
     2881 attctgcaga tatccatcac actggcggcc gctcgagatc tgcgatctaa gtaagcttgg
     2941 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg
     3001 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
     3061 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
     3121 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
     3181 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
     3241 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
     3301 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag
     3361 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc
     3421 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
     3481 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca
     3541 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct
     3601 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc
     3661 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
     3721 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
     3781 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta
     3841 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     3901 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc
     3961 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt
     4021 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     4081 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt
     4141 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     4201 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     4261 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc
     4321 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt
     4381 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     4441 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     4501 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     4561 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa
     4621 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt
     4681 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     4741 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     4801 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     4861 tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaaccc
     4921 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     4981 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg
     5041 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5101 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5161 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5221 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5281 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5341 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5401 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5461 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5521 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5581 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5641 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5701 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5761 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5821 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5881 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5941 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6001 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6061 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6121 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6181 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6241 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6301 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6361 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6421 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6481 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6541 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6601 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6661 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6721 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6781 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6841 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6901 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
