LOCUS       sequence-25428-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
  TITLE     A system for Cre-regulated RNA interference in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
  PUBMED    18779577
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13895-900."
COMMENT     Sequence Label: MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(23..56)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     ncRNA           complement(129..255)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(283..297)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(325..446)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(523..649)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(677..691)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 ctgtacaagt agtctagaag ccataacttc gtatagtaca cattatacga agttatgttt
       61 atgcatacct ggaggaaaaa aaaaagggga gagtcagggg tttaaacgca ttagtcttcc
      121 aattgaaaaa agtgatttaa tttataccat tttaattcag ctttgtaaaa atgtatcaaa
      181 gagatagcaa ggtattcagt tttagtaaac aagataattg ctcctaaagt agccccttga
      241 attccgaggc agtaggcaag gtgaaactat actttacaaa tacatctgtg gcttcactat
      301 ttgtaaagta tagtttcacc gcgctcactg tcaacagcaa tataccttct cgagccttct
      361 gttgggttaa cctgaagaag taatcccagc aagtgtttcc aagatgtgca ggcaacgatt
      421 ctgtaaagta ctgaagcctc attcaacata gtatatgtgc tgccgaagcg agcacttaac
      481 aaggcttgcg gccatttaaa cgcattagtc ttccaattga aaaaagtgat ttaatttata
      541 ccattttaat tcagctttgt aaaaatgtat caaagagata gcaaggtatt cagttttagt
      601 aaacaagata attgctccta aagtagcccc ttgaattccg aggcagtagg catccactac
      661 aagtacatgt gtaatacatc tgtggcttca ctattacaca tgtacttgta gtgggcgctc
      721 actgtcaaca gcaatatacc ttctcgagcc ttctgttggg
//
